Comparative Assessment of ISSR and RAPD Marker Assays for Genetic Diversity Analysis in Jojoba [Simmondsia chinensis (Link) Schneider]

Bhardwaj, Minakshi; Uppal, S.; Jain, Sunita; Kharb, Pushpa; Dhillon, R. S.; Jain, R. K.

doi:10.1007/bf03263351

Cited by 24 publications

(16 citation statements)

References 6 publications

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“…Nei's gene diversity (h) at the ISSR level ranged from 0.10 (DiCA5 0 CR) to 0.37 (DiGA5 0 C), with an average value of 0.19. The PIC values varied from 0.10 for primer DiCA5 0 CR to 0.37 for primer DiGA5 0 C, and the mean PIC value was 0.20 (Table 2), which is similar to values published in the literature for ISSR primers (Bhardwaj et al 2010;Tiwari et al 2009;Zhao et al 2007). Table 1 Genetic Relatedness Among Passion Fruit Accessions Inter-simple sequence repeat results revealed genetic relatedness among the 45 accessions; at about 16% genetic distance, the dendrogram generated using Nei's coefficient resulted in 11 groups (A to K; Fig.…”

Section: Genetic Diversitysupporting

confidence: 54%

ISSR Markers as a Tool for the Assessment of Genetic Diversity in Passiflora

et al. 2011

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Genetic variation among sweet, purple, and yellow passion fruit accessions was assessed using inter-simple sequence repeat (ISSR) markers. Eighteen ISSR primers were used to evaluate 45 accessions. The number of polymorphic bands per primer varied from 4 to 22, with 12.4 bands per primer on average. Nei's genetic distance between accessions ranged from 0.04 to 0.35. Clustering using the neighbor-joining method resulted in the formation of 11 major clusters. It was not possible to classify the accessions according to their geographic origin, showing that there is no structure in the gene bank. The overall mean Shannon-Weaver diversity index was 0.32, indicating good resolution of genetic diversity in passion fruit germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection and to assist breeding and conservation strategies.

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Section: Genetic Diversitysupporting

confidence: 54%

ISSR Markers as a Tool for the Assessment of Genetic Diversity in Passiflora

et al. 2011

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“…This level of polymorphism within the plantain subgroup is considered to be significant. PIC values obtained in both CVGE and CGE analyses of banana are similar to those reported in other crops . In the present study, CVGE analysis resulted in a higher average PIC (0.28) as compared to CGE analysis (0.25), because CGE analysis produced numerous unique bands which resulted in low PIC for each locus.…”

Section: Resultssupporting

confidence: 86%

Comparison of two different electrophoretic methods in studying the genetic diversity among plantains (Musa spp.) using ISSR markers

et al. 2019

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Inter simple sequence repeat markers were employed for the genotyping of 16 plantain ecotypes. Two different electrophoretic systems namely conventional gel electrophoresis (CVGE) and fully automated high‐resolution CGE were used to evaluate the genetic diversity. Comparative analysis indicated that all parameters related to marker informativeness were higher in CGE except polymorphic information content. But genetic diversity parameters like effective number of alleles, Nei's gene diversity (1973) and Shannon's information index showed higher values (1.52 ± 0.12, 0.34 ± 0.05 and 0.52 ± 0.05, respectively) in CVGE as against CGE (1.29 ± 0.04, 0.22 ± 0.02 and 0.38 ± 0.03, respectively) system. The unweighed pair group method with arithmetic averages was used to obtain the dendrogram for both analyses. The results of dendrogram and principal component analysis were found to be consistent in both systems except for some minor disagreements. The clone‐specific bands could be used in the identification and development of SCAR markers. Inter simple sequence repeat markers used in this study provided sufficient polymorphism and reproducible banding pattern for evaluating the genetic diversity of different plantain ecotypes. Lack of accuracy and consistency of the CVGE warrants the employment of high‐throughput CGE for diversity analysis as it provided better separation of bands with higher resolution.

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“…This plant is considered to be drought resistant because it has an extensive and deep root system (Naqvi and Ting 1990). Also, it can be grown on marginal and wasteland due to tolerance of extreme range of temperature from −5 to 54°C (Bhardwaj et al 2010). All these features signify the importance of this commercial crop.…”

Section: Introductionmentioning

confidence: 99%

An efficient and reproducible indirect shoot regeneration from female leaf explants of Simmondsia chinensis, a liquid-wax producing shrub

Bala

Beniwal

Laura

2015

Physiol Mol Biol Plants

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Simmondsia chinensis (Link) Schneider is a perennial, dioecious, drought resistant and multipurpose seed oil crop grown in arid and semi-arid conditions throughout the world. A reproducible and more efficient method for indirect shoot organogenesis from female leaf explants has been standardized. The leaf explants cultured on Murashige and Skoog (MS) medium with 1.0 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) alone produced the highest frequency of callus compared with 1.5 mg l(-1) IBA. Maximum proliferation of callus was observed on MS medium containing a combination of 1.0 mg l(-1) 2,4-D with 0.5 mg l(-1) BAP. For shoot differentiation, the proliferated callus was subcultured on MS medium supplemented with 6-benzylaminopurine (BAP) (1.0-4.0 mg l(-1)) along with 40 mg l(-1) adenine sulphate as additive or in combination with α-naphthalene acetic acid (NAA) or Indole-3-butyric acid (IBA). Optimum shoots differentiated from callus was obtained on MS medium supplemented with 2.0 mg l(-1) BAP and 0.2 mg l(-1) NAA. On this medium, 100 % cultures were responded with an average number of 14.44 shoots per explant with their mean length of 4.78 cm. In vitro rooting (6.22 roots per explant) was achieved on half strength MS medium containing 2 % sucrose with 3.0 mg l(-1) IBA and 300 mg l(-1) activated charcoal (AC). Rooted plantlets were successfully hardened under control conditions and acclimatized under field conditions with 90 % success rate. The present protocol is highly efficient, reproducible and economically viable for large scale production of female plants.

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Comparative Assessment of ISSR and RAPD Marker Assays for Genetic Diversity Analysis in Jojoba [Simmondsia chinensis (Link) Schneider]

Cited by 24 publications

References 6 publications

ISSR Markers as a Tool for the Assessment of Genetic Diversity in Passiflora

ISSR Markers as a Tool for the Assessment of Genetic Diversity in Passiflora

Comparison of two different electrophoretic methods in studying the genetic diversity among plantains (Musa spp.) using ISSR markers

An efficient and reproducible indirect shoot regeneration from female leaf explants of Simmondsia chinensis, a liquid-wax producing shrub

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