The biosorption performance of both batch and liquid-solid fluidized bed operations of dead fungal biomass type (Agaricusbisporus ) for removal of methylene blue from aqueous solution was investigated. In batch system, the adsorption capacity and removal efficiency of dead fungal biomass were evaluated. In fluidized bed system, the experiments were conducted to study the effects of important parameters such as particle size (701-1400µm), initial dye concentration(10-100 mg/L), bed depth (5-15 cm) and solution flow rate (5-20 ml/min) on breakthrough curves. In batch method, the experimental data was modeled using several models (Langmuir,Freundlich, Temkin and Dubinin-Radushkviechmodels) to study equilibrium isotherms, the experimental data followed Langmuir model and the results showed that the maximum adsorption capacity obtained was (28.90, 24.15, 21.23 mg/g) at mean particle size (0.786, 0.935, 1.280 mm) respectively. In Fluidized-bed method, the results show that the total ion uptake and the overall capacity will be decreased with increasing flow rate and increased with increasing initial concentrations, bed depth and decreasing particle size.The pollution of water is one of the main environmental problems facing our planet in the present days due to the increase in the waste of the industrial activities which are often discharged into nature without suitable treatments. Dyes are not biodegradable and their presence in water leads to bioaccumulation in living organisms causing health problems in animals, plants and human beings which require several steps of treatment before draining them into the environment [1]. Dyes are colored materials which can be used in leather, food, paper, textile, printing, and plastics industries. Several methods have been used to remove dyes from the influent water like reverse osmosis, membrane separation, chemical oxidation, coagulation, activated sludge process, and biological treatments; however, the most efficient used method is the adsorption/biosorption method [2,3].The adsorption/ biosorption process has been widely used in wastewater treatments for several reasons such as; it has a simple operating procedure, a relatively small operating space, a capacity for large treatment volumes of effluent, has significant yields, has a large range of feed concentrations, a regeneration of biosorbent, fairly low cost with high efficiency, and an easy scaling-up [4,5]. The decolorization of dyes requires high attention from authorities due to their toxicity, carcinogenic and mutagenic behavior [6]. The methylene blue (MB) was chosen for this study because of its known strong adsorption to solids. MB is the most commonly used material for dying cotton, wood, and silk with a molecular weight of 373.9 that corresponds to MB hydro chlorine with three groups of water [7].At present, physical and chemical methods are widely used as a treatment process for removing dyes from wastewater, but these methods are not cost-effective or environmentally friendly. As a result, an alternative tec...