Comparative characterization and physiological function of putative fatty acid photodecarboxylases

Zeng, Yong-Yi; Yin, Xinjian; Liu, Lan; Zhang, Wuyuan; Chen, Bi‐Shuang

doi:10.1016/j.mcat.2022.112717

Cited by 6 publications

(6 citation statements)

References 29 publications

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“…Each discipline of photobiocatalysis offers opportunities with a varied degree of associated challenges. Although the number of known natural PEs (Figure 1a) is low at the moment, the recent discovery of a new PE-class, namely FAPs [9,[93][94] indicates that there might be more to come, especially as natural light-dependent enzymes were not in the focus of research until recently. Such new enzymes would again trigger an avalanche of opportunities.…”

Section: Outlook Of Using Light In Enzymatic Systemsmentioning

confidence: 99%

Exciting Enzymes: Current State and Future Perspective of Photobiocatalysis

et al. 2023

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The recent increase of interest in photocatalysis spread to biocatalysis and triggered a rush for the development of lightdependent enzyme-mediated or enzyme-coupled processes. After several years of intense research on photobiocatalysis, it is time to evaluate the state of the field in a structured manner. In this Perspective, we suggest to group photobiocatalysis into distinct disciplines and provide principal guidelines and standards for the reporting of photobiocatalytic research results as well as advice on performing photobiocatalytic reactions. Over-all, we assess that the field contributes to the diversity of biocatalytic reactions while offering the selectivity of enzymes to photocatalysis. We foresee that the ongoing excitement for light-dependent enzymatic processes will lead to the discovery of novel photobiocatalytic mechanisms to complement biocatalysis with new bond-forming reactions and will provide additional innovative strategies to utilize light as a possible benign energy source.

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Section: Outlook Of Using Light In Enzymatic Systemsmentioning

confidence: 99%

Exciting Enzymes: Current State and Future Perspective of Photobiocatalysis

et al. 2023

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“…[20,21] In procaryotic expression systems such as Escherichia coli (E. coli) this transit peptide impairs heterologous expression and therefore is generally removed. [22] This natural self-assembly tendency of FAP inspired us to evaluate FAP-immobilization on synthetic membranes. As these membranes are generally chemically inert, they also lack suitable anchoring sites for e. g. covalent enzyme immobilization.…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, wild‐type FAP contains an N ‐terminal transit peptide directing it to the chloroplast membrane [20,21] . In procaryotic expression systems such as Escherichia coli ( E. coli ) this transit peptide impairs heterologous expression and therefore is generally removed [22] . This natural self‐assembly tendency of FAP inspired us to evaluate FAP‐immobilization on synthetic membranes.…”

Section: Introductionmentioning

confidence: 99%

Continuous Fatty Acid Decarboxylation using an Immobilized Photodecarboxylase in a Membrane Reactor

Zhou,

Hollmann,

et al. 2023

ChemSusChem

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The realm of photobiocatalytic alkane biofuel synthesis has burgeoned recently; however, the current dearth of well‐established and scalable production methodologies in this domain remains conspicuous. In this investigation, we engineered a modified form of membrane‐associated fatty acid photodecarboxylase sourced from Micractinium conductrix (McFAP). This endeavour resulted in creating an innovative assembled photoenzyme‐membrane (protein load 5 mg cm−2), subsequently integrated into an illuminated flow apparatus to achieve uninterrupted generation of alkane biofuels. Through batch experiments, the photoenzyme‐membrane exhibited its prowess in converting fatty acids spanning varying chain lengths (C6–C18). Following this, the membrane‐flow mesoscale reactor attained a maximum space‐time yield of 1.2 mmol L−1 h−1 (C8) and demonstrated commendable catalytic proficiency across eight consecutive cycles, culminating in a cumulative runtime of eight hours. These findings collectively underscored the photoenzyme‐membrane's capability to facilitate the biotransformation of diverse fatty acids, furnishing valuable benchmarks for the conversion of biomass via photobiocatalysis.

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“…The potential applications of this enzyme are remarkable, such as in the production of alkane-based biofuels and it has also been demonstrated in the synthesis of other compounds such as chiral secondary fatty alcohols [5]. Mutant variants of CvFAP have also been proposed and studied [6,7], such as other enzymes of FAP group [8,9] and possible ancestral decarboxylases [10].…”

Section: Introductionmentioning

confidence: 99%

New Window of Opportunities for CvFAP photodecarboxylation by Violet Light Irradiation

Mendonça Alves de Souza,

Breda,

França

et al. 2023

Preprint

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In this study, we describe a light-driven photocatalytic decarboxylation of palmitic acid and related fatty acids using Chlorella variabilis fatty acid photodecarboxylase (CvFAP). By utilizing violet LED light (50 W; 397 nm), we achieved a remarkable conversion efficiency of 99% within just 4 minutes, surpassing the previous 79% conversion achieved in 60 minutes using blue LED light (300 W; 439 nm). Importantly, the use of 50 W violet LED light also resulted with lower enzyme photoinactivation rate when compared to 300 W blue LED. Comparing the whole-cell biocatalyst with the enzymatic extract, we found that the former demonstrated superior catalytic performance and reduced susceptibility to photoinactivation. Furthermore, through fed-batch reactions using three pulses of 13 mM palmitic acid, we achieved the production of 39 mM of pentadecane within 1 hour, highlighting a promising strategy for enhanced productivity. These findings represent a significant advancement in CvFAP photodecarboxylation processes, utilizing an alternative light source, with potential implications for biofuel production.

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Comparative characterization and physiological function of putative fatty acid photodecarboxylases

Cited by 6 publications

References 29 publications

Exciting Enzymes: Current State and Future Perspective of Photobiocatalysis

Exciting Enzymes: Current State and Future Perspective of Photobiocatalysis

Continuous Fatty Acid Decarboxylation using an Immobilized Photodecarboxylase in a Membrane Reactor

New Window of Opportunities for CvFAP photodecarboxylation by Violet Light Irradiation

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