Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al. 1982 emend. Lehnen et al. 2006: proposal for Trueperella gen. nov. and emended description of the genus Arcanobacterium

Yassin, Atteyet-Alla Fetouh; Hupfer, H.; Siering, Carsten; Schümann, Peter

doi:10.1099/ijs.0.020032-0

Cited by 164 publications

(141 citation statements)

References 36 publications

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“…Trueperella pyogenes ( T. pyogenes ) [1] is an important opportunistic pathogen in livestock, wild animals, and humans [2]. Pyolysin (PLO), which is secreted by all strains of the bacteria [3], contributes significantly to organism pathogenicity [4,5].…”

Section: Introductionmentioning

confidence: 99%

Replacing the 238th aspartic acid with an arginine impaired the oligomerization activity and inflammation-inducing property of pyolysin

Zhang

Wang

et al. 2018

Virulence

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Trueperella pyogenes (T. pyogenes) is an important opportunistic pathogen. Pyolysin (PLO) importantly contributes to the pathogenicity of T. pyogenes. However, the relationship between the structure and function and the virulence of PLO is not well documented. In the current study, recombinant PLO (rPLO) and three rPLO mutants were prepared. rPLO D238R, a mutant with the 238th aspartic acid replaced with an arginine, showed impairment in oligomerization activity on cholesterol-containing liposome and pore-forming activity on sheep red blood cell membrane. Further study employing the prepared mutants confirmed that the pore-forming activity of PLO is essential for inducing excessive inflammation responses in mice by upregulating the expression levels of IL-1β, TNF-α, and IL-6. By contrast, rPLO P499F, another mutant with impaired cell membrane binding capacity, elicited an inflammation response that was dependent on pathogen-associated molecular pattern (PAMP) activity, given that the mutant significantly upregulated the expression of IL-10 in macrophages and in mice, whereas rPLO did not. Results indicated that domain 1 of the PLO molecule plays an important role in maintaining pore-forming activity. Moreover, the PLO pore-forming activity and not PAMP activity is responsible for the inflammation-inducing effect of PLO. The results of this study provided new information for research field on the structure, function, and virulence of PLO.Abbreviations: T. pyogenes: Trueperella pyogenes; PLO: Pyolysin; rPLO: recombinant PLO; PAMP: pathogen-associated molecular pattern; CDCs: cholesterol-dependent cytolysins; PLY: pneumolysin; NLRP3: NLR family pyrin domain containing protein 3; PRRs: pattern recognition receptors; Asp: aspartic acid; TLR4: Toll-like receptor 4; Arg: arginine; Asn: asparagine; IPTG: Isopropyl-β-d-thiogalactoside; PBS: phosphate-buffered saline; sRBCs: sheep red blood cells; TEM: Transmission electron microscopy; RBCM: red blood cell membrane; SDS-PAGE: sodium dodecyl sulfate–polyacrylamide gel electrophoresis; NC membrane: nitrocellulose membrane; SDS-AGE: dodecyl sulfate agarose gel electrophoresis; MDBK cells: Madin–Darby bovine kidney cells; RPMI-1640 medium: Roswell Park Memorial Institute-1640 medium; FBS: fetal bovine serum; BMDMs: bone marrow-derived macrophages; TNF-α: tumor necrosis factor α; IL-1β: interleukin-1β; IFN-γ: interferon-γ; TGF-β: transforming growth factor-β; ELISA: enzyme-linked immunosorbent assay

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Section: Introductionmentioning

confidence: 99%

Replacing the 238th aspartic acid with an arginine impaired the oligomerization activity and inflammation-inducing property of pyolysin

Zhang

Wang

et al. 2018

Virulence

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“…tococci, as well as Trueperella (T.) pyogenes, formerly classified as Arcanobacterium pyogenes (Ramos et al, 1997;Yassin et al, 2011). Trueperella pyogenes is an opportunistic pathogen that causes suppurative diseases including mastitis, abscesses, pneumonia, and lymphadenitis in economically important food animals such as cows and sheep (Ribeiro et al, 2015).…”

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Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

Alkasir

Wang

Gao

et al. 2016

Acta Veterinaria Hungarica

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Trueperella (T.)pyogenes is an opportunistic pathogen that causes suppurative diseases in domestic animals. In this work, the properties, pathogenesis and phenotypic diversity of T. pyogenes isolates from bovine mastitis were studied. Both pyolysin (plo) and collagen-binding protein (cbp) virulence factor genes were detected by PCR in all T. pyogenes isolates (n = 50). Using the tissue culture plate method, 90% of T. pyogenes isolates were able to form biofilms. The minimum inhibitory concentrations (MICs) of 13 antimicrobials against T. pyogenes isolates were determined. High susceptibility was observed to rifampin (96%), ampicillin (94%), ciprofloxacin (94%), and penicillin (92%), while low susceptibility was found to trimethoprim-sulphamethoxazole (10%) and bacitracin (2%). The intracellular assay revealed that T. pyogenes isolates had different cytopathogenic effects on cells. The high percentage (28.6%) of T. pyogenes isolates suggests that this bacterium is an important contributor to mastitis. Moreover, the high occurrence of multidrug resistance, biofilm production, intracellular survival, and the temporal dynamics of T. pyogenes interactions are key factors for a better understanding of how immunity acts on infections with these bacteria and how they evade immune surveillance, thus highlighting the need for the prudent use of antimicrobial agents in veterinary medicine.Key words: Trueperella pyogenes, bovine mastitis, biofilm, virulence factor, antimicrobial susceptibility Mastitis is an inflammation of the mammary gland caused by several varieties of bacteria such as Staphylococcus (S.) aureus, S. epidermidis, and strep- † Equal contributors

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“…Respecto a las bacterias encontradas, en 3 vacas se aisló E. coli, mientras en solo una A. pyogenes, esto merece un análisis especial, puesto que se ha visto que E. coli puede aumentar la susceptibilidad del endometrio para una posterior infección por A. pyogenes (Gilbert et al 2007;Williams et al, 2007), a la vez A. pyogenes (recientemente reclasificado como (Trueperella pyogenes) (Yassin et al, 2011), puede actuar sinérgicamente con F. necrophorum y P. melaninogenecus aumentando la severidad de la enfermedad uterina (Ruder et al, 1981;Bonnett et al 1991). La especie de E. coli que ocasiona enfermedad uterina es diferente de la E coli patogénica extra intestinal, por lo que se le dio el nombre de E.coli patogénica endometrial (EnPEC), esta se adhiere más fácilmente a las células endometriales y estimula una mayor producción de Prostaglandina E 2 y Interleukina 8 que es el factor quimotáctico para los neutrófilos (Sheldon et al, 2010).…”

Section: Discussionunclassified

Aislamiento e identificación de la flora bacteriana uterina en vacas donantes de embriones

Méndez-Leal¹,

Orjuela

Gómez-Leal

2014

Orinoquia

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ResumenSe aisló e identificó la flora bacteriana en 21 vacas donantes de embriones a partir del lavado uterino al momento de recolección de los embriones al día 7 posinseminación. La identificación bacteriana se realizó por métodos convencionales basados en las características fenotípicas, morfología, desarrollo, pruebas bioquímicas y metabólicas. Las bacterias identificadas fueron Proteus mirabilis 4.8%, Archanobacterium pyogenes 4.8%, Klebsiella ozaenae 9.5%, E. coli 14.2% Shiguella sonnei 23.8%, Bacilus spp 33.3%, Pseudomona aeruginosa 52.3% y Sthaphylococcus spp. 47.6%. En 28.6% de las vacas se identificó solo una bacteria, 52.4% dos bacterias y 19% tres o más bacterias. La alta diversidad bacteriana sugiere diversos orígenes y nuevos estudios sobre su papel individual o sinergismo en el desarrollo de la enfermedad uterina y posibles efectos sobre la respuesta superovulatoria.Palabras clave: infección uterina, bacterias, transferencia de embriones (DeCs). AbstractBacterial flora were isolated and identified in 21 embryo-donor cows from uterine washing when collecting embryos on day 7 post-insemination. Bacteria were identified by conventional methods based on phonotypical and morphological characteristics, development and biochemical and metabolic tests. The bacteria identified were Proteus mirabilis (4.8%), Archanobacterium pyogenes (4.8%), Klebsiella ozaenae (9.5%), E. coli (14.2%), Shiguella sonnei (23.8%), Bacilus spp. (33.3%), Pseudomona aeruginosa (52.3%) and Sthaphylococcus spp. (47.6%). A single bacterium was identified in 28.6% of the cows, two bacteria in 52.4% of the cows and three or more bacteria in 19% of them. The high bacterial diversity suggested various origins; new studies into their individual role and/or synergism regarding the development of uterine disease and possible effects on superovulatory response are needed.

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Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al. 1982 emend. Lehnen et al. 2006: proposal for Trueperella gen. nov. and emended description of the genus Arcanobacterium

Cited by 164 publications

References 36 publications

Replacing the 238th aspartic acid with an arginine impaired the oligomerization activity and inflammation-inducing property of pyolysin

Replacing the 238th aspartic acid with an arginine impaired the oligomerization activity and inflammation-inducing property of pyolysin

Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

Aislamiento e identificación de la flora bacteriana uterina en vacas donantes de embriones

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