Comparative compositional analysis of transgenic potato resistant to potato tuber moth (PTM) and its non-transformed counterpart

Abstract

In this study, the compositions of transgenic potatoes (TPs) resistant to potato tuber moth (Phthorimaea operculella) were compared with those of its non-transgenic (NTP) counterparts. The light inducible promoter, phosphoenolpyruvate carboxylase led to the expression of Cry1Ab only in the leaves and light-treated tubers of the TPs. No significant differences were found in the moisture, ash, dry weight, total soluble protein, carbohydrate, starch, fiber, ascorbate, cations, anions, fatty acids, and glycoalkaloids contents of TP and NTP. Moreover, light treatment significantly affected the contents of ascorbate, acetate and nitrite anions, palmitic, stearic and linolenic fatty acids, α-haconine and α-solanine glycoalkaloids in TP and NTP tubers. While, significant differences were observed in the amino acid contents in light-treated tubers of TPs than the NTP ones. Although, light treatment in potato tubers resulted in marked metabolic changes, all the variations observed in the metabolites compositions were found to be within the desired reference ranges for potato plants. In conclusion, the results indicated that the TPs were substantially and nutritionally equivalent to the NTP counterparts.

Compositional and morphological analysis of salt stress tolerant MTLD- transgenic potato plants

“…The fatty acid analysis showed similar pro les between MTLD-GM and non-GM potato plants, except for stearic acid (Table 4). Earlier studies by Shepherd et al [31] and Rahnama et al [4] reported similar fatty acid pro les for both types of the plants.…”

“…Soluble protein was determined based on the Bradford method [12], while soluble sugar and starch content were assessed by the phenol sulfuric acid method [13]. Soluble sugars were analyzed by High Performance Liquid Chromotograhy (HPLC) (Knauer, Germany) with an IR detector and Eurokat column, with a ow rate of 0.7 mL/min [4]. The extraction and analysis of mannitol content were carried out according to Rahnama et al [6].…”

“…The resulting supernatant was freeze-dried and stored at -20°C for subsequent analysis. The freeze-dried samples were then re-suspended in 1 ml dH 2 O, and their derivation was conducted by adding O-phthaldialdehyde (OPA) reagent [4]. For HPLC analysis, 50 µL 0.5M HCl was added to the derivative samples, and 5 µl of the mixture was injected into the instrument (HPLC Knauer, Germany; HALO C18; uorescent detector: Excitation 330, Emission 450 nM).…”

“…Brie y, 50 mg of dried matter was subjected to extraction using 10 ml HCl 0.1 N (or 10 ml dH 2 O for anion extraction) at a temperature of 80°C for 4 hours. The extract was centrifuged for 10 min at 3000g, and the supernatant was analyzed using Ion chromatography (IC) with a Metrohm Professional IC 850 instrument from, Switzerland [4].…”

“…In the CSA process, the agronomic and phenotypic characteristics of GM crops are evaluated. Additionally, a compositional analysis is conducted to assess essential nutrients, anti-nutrients, toxic compounds, and allergens in food and feed derived from these crops [3,4]. Therefore, CSA represents a practical approach to identifying any signi cant differences between GM crops and non-GM varieties and any biologically signi cant disparities undergo further analysis to assess their potential impact on human health and the environment [2].…”