Comparative cytogenetic mapping of three genes
involved in sex determination in four species of the
family &lt;i&gt;Canidae&lt;/i&gt;

Nowacka‐Woszuk, Joanna; Świtoński, M.

doi:10.22358/jafs/66265/2010

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…The coding region of the SRY gene and exons of SOX9 genes were amplified by polymerase chain reaction (PCR) by means of T personal thermocycler (Biometra, Gottingen, Germany). The sequences of primers were previously described in the studies of Bigliardi, Parma, and Peressotti () ( SRY ) and Nowacka, Nizanski, Klimowicz, Dzimira, and Switonski (), Nowacka‐Woszuk and Switonski () ( SOX9 ; Table ). PCR reaction for SRY gene consisted of initial denaturation at 95°C for 2 min followed by 35 cycles of denaturation at 95°C for 40 s, annealing at 56°C for 50 s and elongation at 72°C for 90 s. Final elongation was carried out at 72°C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

A comprehensive study of disorder of sex development in Staffordshire bull terrier dog

Horňáková

Dianovský

Holečková

et al. 2019

Reprod Domestic Animals

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An 8‐month‐old female Staffordshire bull terrier was clinically examined because of external sexual organs abnormality—clitoral hypertrophy. As stated by the owner, the female dog had not been in heat yet. Serum profile of testosterone (3.39 ng/ml), as well as an anti‐Műllerian hormone (24.0 ng/ml), suggested the presence of testicular tissue. On the contrary, the estimated level of 17β‐oestradiol (24.6 pg/ml) was approximately two times higher when compared with the normal anoestrus values (5–10 pg/ml). A midline laparotomy was performed to detect the cranial parts of the genital system. Gonads resembling testicle or ovotestis (left) and hypoplastic testicle (right) was visible. Cranial portion of gonads was attached to structures indicative of bilateral epididymidis. The next tubular structures—oviducts were resected along with adherent parts of a hypoplastic uterus. Histological evaluation confirmed that the examined gonad samples were testicles with modified interstitial testicular tissue. Hypertrophy of interstitial space was predominantly formed by Leydig cells. Examination of a cross‐section through the head of suspected epididymidis confirmed their characteristic structures. In addition, the characteristic configuration of the oviducts was presented. The uterus consisted of three walls, in which the endometrium was hypoplastic with the presence of endometrial glands. No Y chromosome was detected by chromosomal analysis using CFA Y probe and the amplification of SRY‐gene coding region (813 bp) indicated genotype 78, XX; SRY‐negative. Sequencing of SOX9 gene exons 1–3 did not reveal any differences in exon 1 and 3. On the contrary, a few changes were determined in the SOX9 exon 2 sequences: G instead of A at position 103; C instead of reference T at position 115; GCG instead of reference CGC at position 138–140; T instead of reference C at positions 161, 164 and 167.

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Section: Methodsmentioning

confidence: 99%

A comprehensive study of disorder of sex development in Staffordshire bull terrier dog

Horňáková

Dianovský

Holečková

et al. 2019

Reprod Domestic Animals

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Disorder of sexual development in a Yorkshire terrier (78, XY; SRY-positive)

et al. 2013

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A 9-month-old Yorkshire terrier was admitted to the clinic because of abnormal sexual behaviour and clitoral hypertrophy. External examination confirmed standard development of caudal genital organs: vagina, vulva and cervix uteri. Serum profile of gonadotropin hormones 17 β-estradiol (<10.0 pg.ml(-1)) and testosterone (9.1 ng.ml(-1)) revealed the presence of testicular tissue. A midline laparotomy was performed to detect the cranial parts of the genital system. Gonads resembling testicles, structures indicating epididymis and rudimentary deferent ducts were resected, along with adherent part of the uterus. Cytogenetic analysis showed a male chromosomal complement 78, XY in all metaphases of the studied Yorkshire terrier dog. The chromosomal constitution was confirmed by fluorescence in situ hybridisation (FISH) with whole-chromosome painting probes specific for chromosomes X and Y, as well as by polymerase chain reaction (PCR) amplification of the 271-bp Y-linked fragment of SRY (the sex-determining region on the Y chromosome) gene. Sequencing of the dog's SRY gene coding region did not reveal any mutation. To search for potential mutation in the SOX9 gene (Sry-box containing gene 9), which is considered to be one of the key genes involved in the sex determination process, the PCR fragments of exons 1, 2 and 3 originating from the canine patient were sequenced in order to compare with both male and female healthy control dogs. In the analysed regions of the SOX9 gene, no mutation was found.

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Comparative Cytogenetic Analysis of Sex Chromosomes in Several Canidae Species Using Zoo-FISH

Bugno‐Poniewierska¹,

Sojecka²,

Pawlina³

et al. 2012

folia biol (krakow)

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Comparative cytogenetic mapping of three genes involved in sex determination in four species of the family <i>Canidae</i>

Cited by 3 publications

References 21 publications

A comprehensive study of disorder of sex development in Staffordshire bull terrier dog

A comprehensive study of disorder of sex development in Staffordshire bull terrier dog

Disorder of sexual development in a Yorkshire terrier (78, XY; SRY-positive)

Comparative Cytogenetic Analysis of Sex Chromosomes in Several Canidae Species Using Zoo-FISH

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