Comparative DSC study of human and bovine serum albumin

Michnik, Anna; Michalik, Katarzyna; Kluczewska, Aneta; Drzazga, Z.

doi:10.1007/s10973-005-7170-1

Cited by 127 publications

(69 citation statements)

References 15 publications

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“…An increase in albumin level after the exercise was found in our earlier study [37]. Additionally, conformational changes in albumin molecule due to the binding of fatty acids or other ligands as well as protein glycation affect its folding and stability [41,42]. This heterogeneity of the albumin molecule may cause the extension of thermal unfolding range.…”

Section: Has Beenmentioning

confidence: 68%

Blood serum DSC analysis of well-trained men response to CrossFit training and green tea extract supplementation

Michnik

Sadowska-Krępa

Domaszewski

et al. 2017

J Therm Anal Calorim

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Differential scanning calorimetry (DSC) has been used for the detection of post-exercise changes in blood serum resulting from participation in the CrossFit (CF) training combined with green tea extract (GTE) supplementation. Blood samples from 20 well-trained men were collected at rest, immediately post-exercise and after 1 h of recovery in two trials: first before and second after CF training combined with GTE or placebo administration in the supplemented (S) and control (C) groups, respectively. Selected muscle damage biomarkers have been compared in different phases of the experiment. A significant increase in blood lactate content has been observed post-exercise in both trials in both participants' groups. The opposite trends have been noted for the C and S groups in creatine kinase (CK) activity changes recorded during the first to the second trial: an increase in CK for the control and a decrease for the supplemented group in all phases of the experiment: pre-exercise, post-exercise and after recovery. In the second trial, all CK values for the S group have been found significantly lower than the corresponding values recorded in the C group. These results suggest a mitigate effect of GTE supplementation on post-training muscle damage. DSC results did not reveal clear effects of training nor GTE supplementation on serum denaturation transition. However, interesting dependences of thermodynamic parameters describing this transition have been observed in different phases of the experiment. Statistically significant negative correlations have been found between post-training VO 2max and post-exercise thermodynamic parameters associated with haptoglobin contribution to serum denaturation transition.

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Section: Has Beenmentioning

confidence: 68%

Blood serum DSC analysis of well-trained men response to CrossFit training and green tea extract supplementation

Michnik

Sadowska-Krępa

Domaszewski

et al. 2017

J Therm Anal Calorim

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“…[4][5][6][7][8][9][10][11][12][13][14] The status of bovine serum albumin (BSA) during denaturation has been analyzed and confirmed using far-ultraviolet circular dichroisom. 13,14 Thermal analysis using differential scanning calorimetry (DSC) discloses the thermodynamic conditions under which denaturation occurs and quantifies the enthalpy of denaturation.…”

mentioning

confidence: 99%

“…[4][5][6][7][8]18 It has been reported that DSC studies of BSA reveal the thermodynamics of the protein associated with denaturation, specifically the change in specific heat and enthalpy near the denaturation temperature T d . [6][7][8] However, relatively less attention has been paid to k of BSA (k BSA ). 18 Therefore, we measured k of aqueous solutions of BSA (k sol ) by varying T and pH to determine how denaturation affects thermal transport in the solutions.…”

mentioning

confidence: 99%

Thermal conductivity of bovine serum albumin: A tool to probe denaturation of protein

Park

et al. 2011

Applied Physics Letters

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We demonstrate a strong correlation between denaturation of bovine serum albumin (BSA) and the thermal conductivity k of aqueous solutions of BSA. When denaturation of BSA began, k dropped significantly. These results suggest that k, i.e., the ability of a protein to transport passively applied thermal energy, can be exploited to probe the conformational dynamics of BSA and potentially of other proteins. The technique of protein analysis demonstrated in this work is expected to be useful in micro-total-analysis systems because it is easier to miniaturize and to integrate into a device than is conventional differential scanning calorimetry analysis.

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“…The representative DSC curves of blood serum obtained from 7-and 28-day old newborns ( Averaged absorption spectra of control blood serum for 7-and 28-day old newborn rats although they can differ in their unfolding pathway [15][16][17]. It was shown by Garbet et al [4] that DSC curves of blood plasma represent the sum of weighted contributions of an individual protein.…”

Section: Resultsmentioning

confidence: 99%

Calorimetric and spectroscopic studies characterization of newborn rat’ blood serum after maternal administration of cyclophosphamide

Drzazga

Michalik

Halat

et al. 2010

J Therm Anal Calorim

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Differential scanning microcalorimetry (DSC) and UV-VIS absorption spectroscopy were used to obtain the characteristics of blood serum from newborn rat' after maternal treatment with cyclophosphamide in comparison with control. The obtained DSC curves reveal a complex endothermic peak due to the unfolding process of various serum proteins. Thermal profiles and absorption spectra of blood serum are sensitive to the age of newborns as well as to effect of maternal administration of cyclophosphamide. The most significant disturbances in serum proteome were observed for 14-day old newborns. The thermodynamic parameters: enthalpy change (DH), the normalized first moment (M 1 ) of the thermal transition with respect to the temperature axis and the ratio of C p ex at 70 and 60°C describing denaturation contributions of globulin forms in respect to unliganded albumin with haptoglobin was estimated. Moreover, the second derivative spectroscopy in the UV region was used to resolve the complex protein spectrum. The differences in blood serum detected by DSC and UV-VIS confirm a potential usefulness of these methods for diagnostic and monitoring changes with age as well as the pathological state of blood serum.

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Comparative DSC study of human and bovine serum albumin

Cited by 127 publications

References 15 publications

Blood serum DSC analysis of well-trained men response to CrossFit training and green tea extract supplementation

Blood serum DSC analysis of well-trained men response to CrossFit training and green tea extract supplementation

Thermal conductivity of bovine serum albumin: A tool to probe denaturation of protein

Calorimetric and spectroscopic studies characterization of newborn rat’ blood serum after maternal administration of cyclophosphamide

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