Comparative effects of methylprednisolone and tetracosactide (ACTH 1–24 ) on ischemia/reperfusion injury of the rabbit spinal cord

Abstract

IntroductionTetracosactide is an engineered peptide that applies the same biological impacts as the endogenous adrenocorticotropic hormone. Previous studies indicated that tetracosactide has anti-inflammatory, antioxidant and neurotrophic activity. In this study, we hypothesized that tetracosactide may have protective effects in spinal cord ischemia-reperfusion injury.Material and methodsRabbits were randomized into the accompanying four groups of eight animals each: group 1 (control), group 2 (ischemia), group 3 (methylprednisolone) and group 4 (tetracosactide). In the control group, just a laparotomy was performed. In the various groups, the spinal cord ischemia model was made by the impediment of the aorta only caudal to the renal vein. Neurological assessment was conducted with the Tarlov scoring system. Levels of myeloperoxidase, malondialdehyde and catalase were analyzed, similar to the activities of xanthine oxidase and caspase-3. Histopathological and ultrastructural assessments were additionally performed.ResultsAfter ischemia-reperfusion injury, increments were found in the tissue myeloperoxidase levels (p < 0.001), malondialdehyde levels (p < 0.001), xanthine oxidase action (p < 0.001) and caspase-3 movement (p < 0.001). Conversely, both serum and tissue catalase levels were diminished (p < 0.001 for both). After the administration of tetracosactide, declines were seen in the tissue myeloperoxidase levels (p < 0.001), malondialdehyde levels (p = 0.003), xanthine oxidase action (p < 0.001) and caspase-3 movement (p < 0.001). Conversely, both the serum and tissue catalase levels were expanded (p < 0.001). Besides, tetracosactide treatment indicated enhanced results related to the histopathological scores (p < 0.001), the ultra-structural score (p = 0.008) and the Tarlov scores (p < 0.001).ConclusionsThe findings showed for the first time that tetracosactide shows significant neuroprotective activity against ischemia-reperfusion injury of the spinal cord.

Phosphoglycerate Mutase 1 Prevents Neuronal Death from Ischemic Damage by Reducing Neuroinflammation in the Rabbit Spinal Cord

“…In addition, we examined MDA and 8-iso-PGF2α, which are indicators of the oxidative status [ 33 ]. Spinal cord ischemia significantly increased the MDA and 8-iso-PGF2α levels in the spinal cord, and these results are consistent with previous studies that showed that spinal cord ischemia increased biomarkers of free radical damage [ 5 , 8 , 34 ]. Treatment with Tat-PGAM1, and not Control-PGAM1, significantly ameliorated the increase in MDA and 8-iso-PGF2α levels in the spinal cord.…”

Protective effect of alpha‐lipoic acid against apical periodontitis‐induced cardiac injury in rats

“…Three parameters (congestion, myocyte dissociation, inflammation) of heart tissue were evaluated histopathologically and graded as follows: 0, none; 1, mild; 2, moderate; and 3, severe. The histopathology score of heart tissue of each animal in all groups was the average of these three parameters .…”

The Repression of the HMGB1-TLR4-NF-κB Signaling Pathway by Safflower Yellow May Improve Spinal Cord Injury

“…The ratio of M1 to M2 influences the microenvironment of the spinal cord tissue after injury, as the augmentation of M1 phenotype after SCI will negatively affect the injury repair (Fan et al, 2018). Besides, reactive oxygen species (ROS) can lead to cell and tissue dysfunction through the oxidation of DNA and cell membranes, which further causes inflammation (Hervera et al, 2018;Kertmen et al, 2018). SCI comprises of three stages; acute stage, acute secondary stage, and chronic stage (Nukolova et al, 2018).…”