Comparative effects of sodium dodecyl sulfate and sulfobutyl ether-β-cyclodextrin as pseudostationary phases in the electrokinetic chromatographic separation of hydrophobic compounds

Szücs, Roman; Beaman, Jon V.; Lipczynski, Andrew M.

doi:10.1016/s0021-9673(98)01043-7

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…Capillary electrokinetic chromatography (EKC) extends the application range of CE by providing an approach for separation of neutral as well as ionic solutes . The separation in EKC is based on the partitioning of analytes between buffer solution and pseudostationary phases (PSPs).…”

Section: Introductionmentioning

confidence: 99%

“…[5][6][7] Capillary electrokinetic chromatography (EKC) extends the application range of CE by providing an approach for separation of neutral as well as ionic solutes. [8][9][10] The separation in EKC is based on the partitioning of analytes between buffer solution and pseudostationary phases (PSPs). Although successfully used as PSPs in many separation applications, conventional materials, especially micelles, have some drawbacks as PSPs in EKC.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the enantioselectivity of capillary electrokinetic chromatography using ethanediamine‐bonded poly (glycidyl methacrylate) microspheres as the pseudostationary phases

Sun

Tao

et al. 2019

Chirality

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In this work, a new capillary electrokinetic chromatography (EKC) approach using ethanediamine‐bonded poly (glycidyl methacrylate) (Ami‐PGMA) microspheres as pseudostationary phases (PSPs) for enantioseparation with a polysaccharide, chondroitin sulfate E (CSE), as the chiral selector. The CSE@Ami‐PGMA EKC system was applied to enantioseparate basic drugs, and distinct improved separations of tested enantiomers were obtained while comparing with the single CSE system (the resolution increased from 0.41 to 1.26 for nefopam, from 1.24 to 2.15 for laudanosine, and from 0.92 to 2.36 for amlodipine). The Ami‐PGMA microspheres were fully characterized by scanning electron microscopy (SEM) and Fourier Transform Infrared (FT‐IR) spectroscopy, and the results showed Ami‐PGMA microspheres were uniform and spherical in size (1 μm). Several principal parameters were systematically investigated, and the optimal chiral separations were obtained with Tris/H3PO4 (20 mM, pH 2.4, and 3.4 for NEF) containing 2.5% (w/v) CSE and 20‐μg Ami‐PGMA microspheres in 20°C. Subsequently, the concentrations of Ami‐PGMA microspheres and CSE were proved to be the dominant factors for the separation in the CSE@Ami‐PGMA EKC system by Statistical Product and Service Solutions (SPSS).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of the enantioselectivity of capillary electrokinetic chromatography using ethanediamine‐bonded poly (glycidyl methacrylate) microspheres as the pseudostationary phases

Sun

Tao

et al. 2019

Chirality

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Generic approach to chiral separations: Chiral capillary electrophoresis with ternary cyclodextrin mixtures

Szücs¹,

Caron²,

Taylor³

et al. 2000

J. Micro. Sep.

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A generic approach to chiral separations using capillary electrophoresis is outlined. The generic method was developed by investigating the interactions of charged (sulfobutyl ether‐β‐cyclodextrin, SBE‐β‐CD) and neutral cyclodextrins (primarily γ‐ and dimethyl‐β‐cyclodextrin) in a low pH buffer (50 mM sodium dihydrogen phosphate, pH 2.5). Using a background electrolyte (BGE) containing 5% w/w SBE‐β‐CD, 25 mM γ‐cyclodextrin (γ‐CD) and 25 mM dimethyl‐β‐CD (DM‐β‐CD) dissolved in the phosphate buffer, resolution of more than 90% of the 26 randomly selected racemic pharmaceutical drugs or their synthetic intermediates was achieved. © 2001 John Wiley & Sons, Inc. J Micro Sep 12: 568–576, 2000

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Experimental verification of a predicted, hitherto unseen separation selectivity pattern in the nonaqueous capillary electrophoretic separation of weak base enantiomers by octakis (2,3-diacetyl-6-sulfato)-γ-cyclodextrin

Zhu

Vigh

2000

Electrophoresis

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The capillary electrophoretic separation of cationic enantiomers with single-isomer multivalent anionic resolving agents was reexamined with the help of the charged resolving agent migration model. Three general model parameters were identified that influence the shape of the separation selectivity and enantiomer mobility difference curves: parameter b, the binding selectivity (K(RCD)/K(SCD)), parameter s, the size selectivity (mu0(RCD)/mu0(SCD)), and parameter a, the complexation-induced alteration of the analyte's mobility (mu0(RCD)/mu0). In addition to the previously observed discontinuity in separation selectivity that occurs as mu(eff) of the less mobile enantiomer changes from cationic to anionic, a new feature, a separation selectivity maximum was predicted to occur in the resolving agent concentration range where both enantiomers migrate cationically provided that (i) K(RCD)/K(SCD) <1 and mu0(RCD)/mu0(SCD) >1 and (K(RCD)mu0(RCD))/(K(SCD)mu0(SCD)) > 1, or (ii) K(RCD)/K(SCD) >1 and mu0(RCD)/mu0(SCD) <1 and (K(RCD)mu0(RCD))/(K(SCD)mu0(SCD)) <1. This hitherto unseen separation selectivity pattern was experimentally verified during the nonaqueous capillary electrophoretic separation of the enantiomers of four weak base analytes in acidic methanol background electrolytes with octakis(2,3-diacetyl-6-sulfato)-gamma-cyclodextrin (ODAS-gammaCD) as resolving agent.

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Comparative effects of sodium dodecyl sulfate and sulfobutyl ether-β-cyclodextrin as pseudostationary phases in the electrokinetic chromatographic separation of hydrophobic compounds

Cited by 5 publications

References 17 publications

Evaluation of the enantioselectivity of capillary electrokinetic chromatography using ethanediamine‐bonded poly (glycidyl methacrylate) microspheres as the pseudostationary phases

Evaluation of the enantioselectivity of capillary electrokinetic chromatography using ethanediamine‐bonded poly (glycidyl methacrylate) microspheres as the pseudostationary phases

Generic approach to chiral separations: Chiral capillary electrophoresis with ternary cyclodextrin mixtures

Experimental verification of a predicted, hitherto unseen separation selectivity pattern in the nonaqueous capillary electrophoretic separation of weak base enantiomers by octakis (2,3-diacetyl-6-sulfato)-γ-cyclodextrin

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