Comparative efficacy and toxicity of a ribosomal vaccine, acetone-killed cells, lipopolysaccharide, and a live cell vaccine prepared from Salmonella typhhimurium

Angerman, C R; Eisenstein, Toby K.

doi:10.1128/iai.19.2.575-582.1978

Cited by 60 publications

(20 citation statements)

References 30 publications

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“…These observations raised the possibility that the early appearing 3///~ T cells during infection with facultative intracellular microorganisms may serve as a first defense against microbial pathogens. Salmonella sp are also facultative intracellular pathogens, and cellular immunity is regarded as one of the most important defense mechanisms, although humoral immunity is also involved in the protection against Salmonella sp (44)(45)(46). Our data presented here indicate that the 3//8 T calls appear in the peritoneal cavity and liver at an early stage after intraperitoneal infection with an avirulent strain of S. choleraesuis.…”

Section: Discussionmentioning

confidence: 59%

Induction of gamma/delta T cells in murine salmonellosis by an avirulent but not by a virulent strain of Salmonella choleraesuis.

Emoto

Danbara

Yoshikai

1992

The Journal of Experimental Medicine

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To elucidate the relationship between the virulence of intracellular bacterium and its ability to induce gamma/delta T cells in the host during infection, we examined the differences in appearance of gamma/delta T cells in mice infected with Salmonella choleraesuis virulent strain RF-1 carrying a virulence plasmid of 50 kb, and with avirulent strain 31N-1 cured of the 50-kb plasmid. The number of gamma/delta T cells in the peritoneal cavity was increased to a significant level on day 3 after an intraperitoneal infection with a sublethal dose (5 x 10(4) colony-forming units) of avirulent strain 31N-1. On the other hand, no increase in the number of gamma/delta T cells was evident in the peritoneal cavity at any stage after infections with various doses of virulent strain RF-1, although the numbers of the bacteria were drastically increased. Similar to that seen in the peritoneal cavity, the number of gamma/delta T cells in the liver was significantly increased after an intraperitoneal infection with avirulent strain 31N-1 but not with virulent strain RF-1. The early appearing gamma/delta T cells during salmonellosis with avirulent stain 31N-1, which preferentially used V gamma 1/V delta 6, showed blastogenesis in response to purified protein derivative (PPD) derived from Mycobacterium tuberculosis. The gamma/delta T cells also responded to the peritoneal adherent cells in mice infected with avirulent strain 31N-1 6 d previously, which expressed a high level of endogenous heat-shock protein (hsp) homologous to the mycobacterial 65-kD hsp. The expression of the hsp, however, was not prominent in the adherent cells in mice infected with virulent strain RF-1. These results suggest that the gamma/delta T cells specific for PPD may play important roles in host defense against murine salmonellosis, and that the virulence of Salmonella may be inversely correlated with its ability to induce endogenous hsp in the infected macrophages, which in turn stimulate the gamma/delta T cells in the host during salmonellosis.

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Section: Discussionmentioning

confidence: 59%

Induction of gamma/delta T cells in murine salmonellosis by an avirulent but not by a virulent strain of Salmonella choleraesuis.

Emoto

Danbara

Yoshikai

1992

The Journal of Experimental Medicine

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“…However, the role of antibodies during different stages of infection, and particularly in protection, is still incompletely understood, despite the large number of results published on this matter [2]. Vaccination of resistant mice with either attenuated or killed Salmonellae induced protection against secondary infection with an otherwise lethal dose of virulent bacteria and this protection could be passively transferred to naive mice with serum [79][80][81]. In susceptible mice, however, the situation is different.…”

Section: B Cells and Antibodies In Immunity Against S Typhimuriummentioning

confidence: 99%

Immune response to infection with Salmonella typhimurium in mice

Mittrücker

Kaufmann

2000

Journal of Leukocyte Biology

254

213

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Infection of mice with Salmonella typhimurium results in systemic infection and a disease similar to that seen in humans after infection with S. typhi. The innate immune system can restrict replication of S. typhimurium to a certain degree, but for effective control and eradication of bacteria, acquired immunity is essential. Salmonella infection induces the generation of specific CD4 ؉ and CD8 ؉ T cells, and both T cell populations are important for protection during primary and secondary responses, although the mechanisms underlying T cell-mediated protection are not yet completely understood. Infection with S. typhimurium also results in a strong antibody response to Salmonella antigens and, in contrast to most other intracellular bacteria, this antibody response participates in protection. In summary, the response to S. typhimurium involves both T and B cell-mediated immunity, and mechanisms mediated by both lymphocyte populations are important for control of primary infection and protection against secondary infection.

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“…Many investigators indicated that the efficacy of salmonella ribosomal vaccines varies with mouse strains [2], and that contaminating lipopolysaccharide (LPS) exerted the major effect [3,4]. In addition, salmonella ribosomal vaccines can induce mouse protection comparable to that induced by immunization with 230 a sublethal dose of viable virulent S. typhimurium [5,6] or with viable cells of attenuated strains [7].…”

Section: Introductionmentioning

confidence: 99%

Requirement of the conformational stability of a Salmonella ribosomal vaccine for its mouse protection

Kita

1991

FEMS Microbiology Letters

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Summary The 43‐kDa non‐O antigenic component isolated from the crude ribosomal fraction of Salmonella typhimurium [9] was further purified by affinity chromatography (43‐kDa protein: 43‐kDp). Immunization with 43‐kDp did not induce complete mouse protection in CF1 mice to 500 LD50 of S. typhimurium, although it elicited a substantial IgG antibody response. The 43‐kDp exhibited the mitogenicity to splenocytes (CF1 and C3H/HeJ) and B cell‐rich populations (CF1). Complexing 43‐kDp with the compact ribosomes of Streptococcus pyogenes by formaldehyde (complex vaccine: CV) elicited both IgM and IgG antibodies to 43‐kDp. CN induced a boosting effect to enhance IgG antibody response. Moreover, CV generated delayed‐type hypersensitivity to salmonella antigens and also conferred complete protection against 500 LD50 challenge of S. typhimurium to CF1 mice. These abilities of CV were reduced or impaired by RNase digestion. CV was able to induce partial or complete protection in inbred mouse strains (C3H/HeN, C3H/HeJ, DBA/2 and A/J). These data, in addition to other reports, suggest that conformational stability between ribosomes and contaminating substances such as 43‐kDp or O‐antigens might be required for the overall effects of the ribosomal vaccine.

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Comparative efficacy and toxicity of a ribosomal vaccine, acetone-killed cells, lipopolysaccharide, and a live cell vaccine prepared from Salmonella typhhimurium

Cited by 60 publications

References 30 publications

Induction of gamma/delta T cells in murine salmonellosis by an avirulent but not by a virulent strain of Salmonella choleraesuis.

Induction of gamma/delta T cells in murine salmonellosis by an avirulent but not by a virulent strain of Salmonella choleraesuis.

Immune response to infection with Salmonella typhimurium in mice

Requirement of the conformational stability of a Salmonella ribosomal vaccine for its mouse protection

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