Comparative Efficacy of dsRNA VP24, VP26, RR1 and WSV477  Gene against WSSV Infection in Penaeus monodon

Puneeth, G.; Akhila, Dharnappa Sannejal; Dechamma, Mundanda Muthappa; Shreeharsha, J.M.B.F.Sc; Shivakumar, Santhosh Kogaluru; Venugopal, M. N.

doi:10.20546/ijcmas.2017.602.075

Cited by 8 publications

(7 citation statements)

References 23 publications

(28 reference statements)

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“…The shrimp survival by injected with dsRNA of VP24, VP19 and VP15 (Sarathi et al, 2010) and VP28 (Nilsen et al, 2017;Sarathi et al, 2008;Solis-Lucero et al, 2016) was significantly higher than that of control groups. The survival enhancement of tiger shrimp by 50% has also been reported by Puneeth et al (2017) using the VP24 vaccine by a dose of 2.5 µg/g of body weight.…”

Section: Survival Ratesupporting

confidence: 56%

“…Preparation of feed containing dsRNA was carried out by referring to Puneeth et al (2017). A total of 0.02 g of commercial shrimp pellet (a protein content of 30%) was mixed with 10 8 cells of inactivated bacteria, then incubated on ice for 15 minutes and then at room temperature for 30 minutes to allow absorption, before being coated with fish oil to prevent dispersion.…”

Section: Administration Of Oral Vaccination and Wssv Challenge Testmentioning

confidence: 99%

“…A study of the dsRNA VP-24 vaccine at a dose of 0.2 μg showed a significant increase in survival rate in tiger shrimp by 65% compared to control (10%) (Mulyaningrum et al, 2018). Some studies also demonstrated that the intramuscular injection and oral delivery of dsRNA can provide better protection than control groups of tiger shrimp (Puneeth et al, 2017) and our studies indicated that the application of dsRNA by injection showed a better survival response than oral administration (Mulyaningrum et al, 2018;Parenrengi et al, 2018;2019). The shrimp survival by injected with dsRNA of VP24, VP19 and VP15 (Sarathi et al, 2010) and VP28 (Nilsen et al, 2017;Sarathi et al, 2008;Solis-Lucero et al, 2016) was significantly higher than that of control groups.…”

Section: Survival Ratementioning

confidence: 99%

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Application of dsRNA VP15-WSSV by Oral Vaccination to Increase Survival Rate and Response Immunes of Tiger Shrimp Penaeus monodon

Parenrengi

Tenriulo

Suryati

et al. 2022

IJAR

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Background: RNA interference (RNAi) has recently introduced as a powerful technique for specific gene silencing in antiviral therapy for controlling shrimp diseases. The present study was conducted to assess the effect of dsRNA VP15-WSSV vaccine by oral administration on tiger shrimp survival rate and response immunes. Methods: A gene encoding VP15 was isolated from tiger shrimp infected WSSV and cloned into the L4440 vector with the T7 promoter. For mass production, the pT7-VP15 gene construct was transformed to bacteria. The bacteria were inactivated using the heat-killed method by immersion and 108 cells of bacteria were mixed with 0.02 g of commercial shrimp feed for dsRNA application. The shrimp were fed by: (A) feed containing dsRNA and coated by fish oil, (B) feed without dsRNA and coated by fish oil and (C) feed without dsRNA and fish oil. The shrimp were challenged with WSSV by intramuscular injection after vaccination for two weeks. The survival rate (SR) after the challenge test was counted daily, while the THC and proPO were observed before, then on 1st, 3rd and 5th days post-challenge (dpc). Result: The survival rate of tiger shrimp for all treatments tended to decrease until the 3rd dpc, while the 4th dpc the vaccinated tiger shrimp was started to exhibit the higher SR compared to both treatments. The vaccinated tiger shrimp had significantly (P less than 0.05) higher SR and THC compared with both control treatments, but not significantly (P greater than 0.05) for proPO activity. The results indicated that the application of oral dsRNA vaccination suggested increasing in SR and response immune of tiger shrimp.

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Section: Survival Ratesupporting

confidence: 56%

Section: Administration Of Oral Vaccination and Wssv Challenge Testmentioning

confidence: 99%

Section: Survival Ratementioning

confidence: 99%

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Application of dsRNA VP15-WSSV by Oral Vaccination to Increase Survival Rate and Response Immunes of Tiger Shrimp Penaeus monodon

Parenrengi

Tenriulo

Suryati

et al. 2022

IJAR

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“…Di bidang akuakultur, teknologi ini banyak dikembangkan untuk menanggulangi penyakit yang disebabkan oleh virus pada udang (Mejía-Ruíz et al, 2011;Ahanger et al, 2014). Beberapa penelitian vaksinasi udang menggunakan agen WSSV sebagai sumber vaksin telah menunjukkan kemajuan dan memperoleh hasil yang menggembirakan (Huang et al, 2014;Kumar et al, 2015;Puneeth et al, 2017).…”

Section: Pendahuluanunclassified

“…Sarathi et al (2010) memperoleh mortalitas komulatif udang windu P. monodon sebesar 37% dengan injeksi dsRNA VP-24. Sintasan yang diperoleh pada penelitian ini lebih tinggi dari hasil yang diperoleh oleh Puneeth et al (2017) yang memperoleh sintasan udang windu sebesar 50% dengan injeksi vaksin VP-24 in vivo dengan dosis 2,5 µg/g bobot udang.…”

Section: Sintasan Udang Winduunclassified

RESPONS IMUN UDANG WINDU Penaeus monodon TERHADAP VAKSIN dsRNA VP-24 PADA DOSIS BERBEDA

Mulyaningrum

Parenrengi

Tampangallo

et al. 2018

Jurnal Riset Akuakultur

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Peningkatan produksi udang windu Penaeus monodon terus diupayakan, salah satunya dengan peningkatan respons imun udang terhadap infeksi penyakit WSSV. Penelitian ini bertujuan untuk mengetahui respons imun udang terhadap pemberian vaksin dsRNA VP-24 pada berbagai dosis. Konstruksi vaksin dsRNA VP-24 dilakukan menggunakan Megascript kit dengan DNA genom VP-24 sebagai template. Vaksinasi dilakukan dengan metode injeksi pada udang windu yang berukuran rata-rata 15,88 ± 3,50 g; dosis vaksin yang diujikan adalah 0,02 µg; 0,2 µg; 2 µg; dan sebagai kontrol adalah udang yang tidak diberi vaksin. Penelitian terdiri atas empat perlakuan dosis vaksin dengan masing-masing dua ulangan dan dipelihara selama lima hari. Uji tantang dilakukan selama enam hari dengan menginjeksi virus WSSV dalam saline solution (1:3 v/v). Pengamatan terhadap sintasan udang windu dilakukan setiap hari, sedangkan penghitungan total hemocyte (THC) dan ProPO diamati pada hari I, III, dan VI setelah diinfeksi WSSV. Pada akhir pengujian dilakukan pengambilan jaringan hepatopankreas untuk analisis histopatologi. Analisis data dilakukan secara statistik dengan analisis ragam (ANOVA). Hasil yang diperoleh memperlihatkan bahwa injeksi vaksin dsRNA VP-24 dengan dosis 0,2 µg memiliki pengaruh yang signifikan terhadap sintasan dan respons imun udang windu (P<0,05). Vaksin dsRNA VP-24 dengan dosis 0,2 µg mampu memberikan sintasan udang windu P. monodon sebesar 65% dan meningkatkan respons imun udang dengan THC (1.550 x 10t sel/mL) dan ProPO (0,042 Abs).One of the efforts to increase the production of tiger shrimp Penaeus monodon is increasing the immune response against WSSV disease. This study aims to evaluate shrimp immune response to dsRNA VP-24 vaccination at various doses. The construction of dsRNA VP-24 vaccine was performed using Megascript kit with the VP-24 DNA genome as a template. The vaccination was done by injection method on shrimp sized 15.88 ± 3.50 g. The tested vaccine doses (treatments) were 0.02 ¼g; 0.2 ¼g; 2 ¼g; and unvaccinated shrimp as the control. The study consisted of four treatments of vaccine doses with two replicates for each treatment. The challenge test was performed by injecting the WSSV virus in saline solution (1:3 v/v). The observation on shrimp survival rate was done daily, while the total hemocyte count (THC) and ProPO observation were performed on the 1st day, 3rd day, and 6th day after WSSV infection. At the end of the experiment, samplings of hepatopancreas for analysis were performed. Data were statistically analyzed using ANOVA. The present study indicated that the injection of 0.2 ¼ g dsRNA VP-24 vaccine had a significant effect on the survival rate and immune response of shrimp (P<0.05). The dose of 0.2 ¼g dsRNA VP-24 had resulted in 65% of survival rate and increased immune response of P. monodon with THC (1,550 x 10t cell/mL) and ProPO (0.042 Abs).

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Prospects of Vaccination in Crustaceans with Special Reference to Shrimp

K.,

Kulkarni,

K. V.

2022

Fish Immune System and Vaccines

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Comparative Efficacy of dsRNA VP24, VP26, RR1 and WSV477 Gene against WSSV Infection in Penaeus monodon

Cited by 8 publications

References 23 publications

Application of dsRNA VP15-WSSV by Oral Vaccination to Increase Survival Rate and Response Immunes of Tiger Shrimp Penaeus monodon

Application of dsRNA VP15-WSSV by Oral Vaccination to Increase Survival Rate and Response Immunes of Tiger Shrimp Penaeus monodon

RESPONS IMUN UDANG WINDU Penaeus monodon TERHADAP VAKSIN dsRNA VP-24 PADA DOSIS BERBEDA

Prospects of Vaccination in Crustaceans with Special Reference to Shrimp

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