Comparative efficacy of FSH-P and PMSG on superovulation in Pashmina goats

Mahmood, S.; Koul, G. L.; Biswas, J.C.

doi:10.1016/0093-691x(91)90365-k

Cited by 27 publications

(11 citation statements)

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“…In this study, the super-ovulation treatment started on the 16 th day of the cycle without intravaginal progestagen treatment and PGF 2 α was administered at a later hour (48 and 60) after the 1 st injection of gonadotropins resulting in a mean ovulation rate of 13.07 in 14 does, while 3 did not respond to treatment. The resulting oestrous and ovulation response (82.3%) was comparable to the rate obtained in most goat breeds so far studied (Armstrong et al, 1983;Mahmood et al, 1991;Cognie et al, 2003) including that of indigenous Feral goat in South Africa (Greyling et al, 2002). Similarly, the mean number of structures recovered per doe (9.9 including unfertilized oocytes and embryos) or 9.7 embryos per doe following flushing was comparable to that obtained in the Boer (Lehloenya et al, 2006a;2008) when using long progestagen treatment (eg., CIDR/PGF 2 α/FSH or CIDR/FSH for 17 days) than when using CIDR for only 7 days.…”

Section: Discussionsupporting

confidence: 52%

Live birth after transfer of vitrified embryos from superovulated goats

2016

Res. Opin. Anim. Vet. Sci.

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In this study, the viability of vitrified goat embryos recovered from superovulated nondescript Philippine goats were evaluated post-warming by transferring to surrogate does. Of 17 does treated for superovulation, 3 did not respond and 14 responded with a 13.07 mean ovulation rate. An average of 9.7 embryos per doe was collected at different developmental stages. Using a container-less minimum drop size (MDS) method of vitrification, 50 and 64 embryos were exposed to vitrification solution 1 (VS1) containing 10% ethylene glycol in basic medium (BM) consisting of Hepes buffered TCM-199 medium + 20% estrus-doe serum solution for 3 min and 10 min, respectively. Immediately thereafter, the embryos were transferred to 40% EG + 1 M sucrose in BM for 45 Sec before recovering and dropping directly into LN 2. After 3 months of storage, the embryos were warmed in 2 ml of 0.3 M sucrose in BM for 5 min and washed twice before transferring into culture medium. Morphologically normal embryos were then transferred surgically to 6 recipients. Seven kids were born after a normal gestation period with birthweights ranging from 0.78-2.5 kg. The results highlight the usefulness of the MDS method of vitrification using an ethylene glycol based solution in the cryopreservation of goat embryos. That, continuous research effort should be made for optimizing cryopreservation protocols for conservation of animal genetic resources. Keywords: Embryo transfer; live birth; superovulation; vitrification To cite this article: Ocampo MB, JQ Silvestre, VD Viernes and LC Ocampo, 2016. Live birth after transfer of vitrified embryos from superovulated goats. Res. Opin. Anim. Vet. Sci., 6(2): 47-52.

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Section: Discussionsupporting

confidence: 52%

Live birth after transfer of vitrified embryos from superovulated goats

2016

Res. Opin. Anim. Vet. Sci.

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“…The increased number of unfertilised ova may have been attributed to either fertilisation failure due to incompetent ova produced or an untimed AI -as the duration of the induced oestrous period was longer in one shot FSH plus eCG protocol (Group 2). Usually incompetent ova are associated with high blood oestrogen levels after ovulation from follicles which did not ovulate when eCG is used as a superovulation agent (Armstrong et al, 1983, Mahmood et al, 1991and Saharrea et al, 1998. In sheep, one shot protocol (simplified) tended to lead to the occurrence of large follicles observed during embryo flushing (Simonetti et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, eCG is usually administered as a single injection, due to its long halflife, approximately 48 h before progestagen removal (Espinosa-Marquez et al, 2004 andHoltz, 2005). When comparing the efficiency of FSH and eCG as superovulation agents in goats, it has been reported that FSH produced higher ovulation and embryo recovery rates, as well as more transferable embryos, although more variable than eCG (Mahmood et al, 1991 andGoel andAgrawal, 2005). Although the utilisation of FSH in the superovulation programme of goats has demonstrated the ability to produce better results, this regime is more labour intensive and imposes more stress to the animals, due to excessive handling -as it must be administered twice daily over a 4 day period.…”

Section: Introductionmentioning

confidence: 99%

Preliminary results evaluating a simplified superovulation protocol in Boer goats

Lehloenya

2013

Small Ruminant Research

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This study was conducted to evaluate the use of one shot follicle stimulating hormone (FSH) plus equine chorionic gonadotrophin (eCG) (simplified) superovulation protocol in mature Boer goat does. Eighteen does used were allocated into two groups, during the natural breeding season (autumn). The two group were Group 1: the control (n = 9); multiple FSH protocol and Group 2: treatment group (n = 9); one shot FSH plus eCG protocol. All does were synchronised for oestrus, using controlled internal drug release dispensers (CIDR's) for 9 days. Does in the multiple FSH protocol group were superovulated with 200 μg pFSH/doe, administered i.m. in 7 dosages at 12 h intervals, starting 48 h prior to CIDR removal. In the treatment group (one shot FSH plus eCG protocol), the superovulation treatment was administered once as 200 μg FSH plus 350 IU eCG, 48 h prior to CIDR removal. All does were then observed for oestrous behaviour twice daily, at 12 h intervals following CIDR removal for a period of 72 h. Cervical inseminations (with 0.1 ml fresh undiluted Boer goat semen) were performed 36 h and 48 h following CIDR removal. Embryos were then surgically flushed on day 6 following the second artificial insemination. The time interval from CIDR removal to onset of oestrus was not affected by treatment. The duration of the oestrous period however was significantly longer in the one shot group, compared to the multiple FSH protocol. Does from the one shot FSH plus eCG protocol recorded a significantly (p < 0.05) lower mean number of CL's, structures (unfertilised ova plus embryos) and (p < 0.01) embryos recovered. A significantly (p < 0.01) higher number of unfertilised ova were observed from the one shot FSH plus eCG group (4.06 ± 0.9), compared to the multiple FSH group (0.24 ± 0.1). However, the number of degenerated embryos was not affected by treatment. The total number of transferable embryos was significantly lower in the one shot FSH plus eCG protocol (0.22 ± 0.2), compared to the multiple FSH protocol (9.56 ± 1.9). The size of follicles was not affected by treatment, however the total number of follicles at CIDR removal was significantly lower in the one shot FSH plus eCG group (11.67 ± 0.7), compared to the multiple FSH group (14.83 ± 0.7). AI might have been performed at the wrong time, because of the duration of the oestrous period being longer in Group 2. The multiple FSH superovulation protocol overall seemed to be more efficient in the Boer goat. More research is needed on the doses of gonadotrophins administered and the time of AI, in order for the simplified protocol (Group 2) to be effective.

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“…The results regarding the The response to superovulation as reflected by ovulation rate, total number of structures and embryos collected was lower in the maiden young does. In both sheep and goats poorer superovulatory responses have been recorded in younger females (less than three years of age) (Torres et al, 1987, Mahmood et al, 1991Dingwall et al, 1993;Lopes et al, 2006). Several factors may contribute to this tendency.…”

Section: Discussionmentioning

confidence: 99%

“…There is a general agreement that pre-pubertal and young (age, 1 -2 years) animals can be superovulated and these follicles are also sensitive to gonadotrophin stimulation (Donaldson, 1984;Driancourt et al, 1990;Rangel-Santos et al, 1991;Hasler, 1992;Kuhholzer & Brem, 1999). Although the ovaries of young females can respond to external gonadotrophins administered, there have been incidences of a poorer ovarian response to superovulation (in terms of ovulation and fertilisation rates), as well as lower embryo recovery and survival rates recorded, compared to multiparous or adult females (Quirke & Hanrahan, 1977;Mahmood et al, 1991;Rangel-Santos et al, 1991;Driancourt & Avdi, 1993 (Quirke & Hanrahan, 1977;Wright et al, 1981;McMillan & McDonald, 1985). It was also reported that adult ewes yield higher ovulation rates compared to young ewe (aged, 1 -2 years) (Baril et al, 2000;Lopes et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

The effect of embryo donor age and parity on the superovulatory response in Boer goat does

Lehloenya

Greyling

2010

SA J. An. Sci.

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This study was conducted to evaluate the effect of the age and parity of the embryo donor on the superovulatory response and embryo recovery rate in Boer goat does. The oestrous cycles of seven maiden does (young, 1 -2 years) and nine multiparous does (adult, 3 -4 years) were synchronised using controlled internal drug release dispensers (CIDR's) for a period of 17 days, and superovulated with pFSH during the natural breeding season. The superovulation treatment was administered as a total dose of 200 mg pFSH/doe given i.m. in seven dosages (at 12 h intervals) -starting 48 h prior to CIDR removal. Does were observed for oestrous behaviour three times daily, at 8 h intervals following CIDR withdrawal. Cervical inseminations (0.01 mL fresh undiluted semen) were performed 36 h and 48 h following CIDR removal and the embryos surgically flushed six days following the second artificial insemination. All does showed overt signs of oestrus. The time interval from CIDR removal to the onset of oestrus in the adult multiparous does (24.0 ± 4.0 h) was significantly shorter than that recorded in the younger does (32.0 ± 4.6 h). The age and parity of the embryo donors did not have any effect on the duration of the induced oestrous period. The mean number of CL's, structures and embryos recovered were significantly higher in the adult multiparous does (19.8 ± 4.8, 21.3 ± 3.9 and 20.9 ± 4.5, respectively), compared to the young maiden does (13.7 ± 3.8, 11.7 ± 5.0 and 11.7 ± 5.0, respectively). The fertilisation rate, the mean number of unfertilised ova and degenerate embryos recorded did not differ between the young and adult multiparous does. The mean number of transferable embryos in the adult does (15.8 ± 6.4) was, however, significantly higher than in the young does (9.5 ± 3.7). The longer response time taken to the onset of oestrus had no influence on the fertilisation rate in young does. Although the young does recorded an acceptable fertilisation rate, the number of transferable embryos was, however, lower due to the lower total number of embryos produced by the young donor does and this may favour the use of older multiparous does as embryo donors in a multiple ovulation and embryo transfer programme.

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Comparative efficacy of FSH-P and PMSG on superovulation in Pashmina goats

Cited by 27 publications

References 4 publications

Live birth after transfer of vitrified embryos from superovulated goats

Live birth after transfer of vitrified embryos from superovulated goats

Preliminary results evaluating a simplified superovulation protocol in Boer goats

The effect of embryo donor age and parity on the superovulatory response in Boer goat does

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