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Aim: Present study was carried out to study the association of seminal vesicle weight with steroidal (testosterone and estrogen) concentration in buffalo bulls. Materials and Methods:A total of 30 pairs of seminal vesicles were collected from buffalo bulls (irrespective of age and breed) and categorized into group I (<5 gm), group II (5-8 gm) and group III (>8 gm) on the basis of mean seminal vesicles weight for establishing relationship. Results:The mean concentration of testosterone in seminal vesicle fluid showed, significantly (p<0.05) higher value in group III (0.54±0.09 ng/ml) compared to group I (0.33±0.03ng/ml), however, difference between group III and II (0.51±0.05 ng/ml) was non-significant. Similarly, mean estrogen concentration also showed a significantly (p<0.05) higher value in group III (16.31±4.70 pg/ml) compared to group I (2.51±1.09 pg/ml) but values in group III and I were non-significant with group II (8.99±2.55 pg/ml). Conclusion:Present study indicated positive correlation between seminal vesicle weight with testosterone (r=0.365, P< 0.05) and estrogen (r=0.528, P<0.01) concentration respectively in seminal vesicle fluid.

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