Comparative efficacy of hospital disinfectants against nosocomial infection pathogens

Tapouk, Fahim Amini; Nabizadeh, Ramin; Mirzaei, Nezam; Jazani, Nima Hosseini; Yousefi, Mahmood; Hasanloei, Mohamad Amin Valizade

doi:10.1186/s13756-020-00781-y

Cited by 32 publications

(17 citation statements)

References 22 publications

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“…Nutrient broth without disinfectant was inoculated with the bacteria and used as the positive control, while nutrient broth inoculated with the same volume of deionized water was used as the negative control. All the tubes were incubated at 35 °C for 48 h before the results were obtained [ 32 , 33 , 34 , 35 , 36 ]. The MICs of the disinfectants against CRKP strains demonstrated the highest dilution for aseptic growth.…”

Section: Methodsmentioning

confidence: 99%

Disinfection Strategies for Carbapenem-Resistant Klebsiella pneumoniae in a Healthcare Facility

Zhang

Shen

et al. 2022

Antibiotics

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Disinfectant resistance is evolving into a serious problem due to the long-term and extensive use of disinfectants, which brings great challenges to hospital infection control. As a notorious multidrug-resistant bacterium, carbapenem-resistant Klebsiella pneumoniae (CRKP) is one of the most common and difficult pathogens of nosocomial infection. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests of seven kinds of disinfectants (0.1% benzalkonium bromide, 4% aqueous chlorhexidine, 75% alcohol, entoiodine II, 2% glutaraldehyde, 2000 mg/L chlorine-containing disinfectants, and 3% hydrogen peroxide) were detected by the broth dilution method. Three efflux pump genes (oqxA, oqxB, and qacE∆1-sul1) were detected by PCR. The mean MIC value of aqueous chlorhexidine from the intensive care unit (ICU) (0.0034%) was significantly higher than that from non-ICUs (0.0019%) (p < 0.05). The positive rates of three efflux pump genes oqxA, oqxB and qacE∆1-sul1 were 60.9% (39/64), 17.2% (11/64) and 71.9% (46/64) in the detected CRKP isolates, respectively. This study discovered that CRKP strains demonstrated extensive resistance to clinical disinfectants and suggest that it is necessary to perform corresponding increases in the concentration of aqueous chlorhexidine and chlorine-containing disinfectants on the basis of current standards in the healthcare industry.

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Section: Methodsmentioning

confidence: 99%

Disinfection Strategies for Carbapenem-Resistant Klebsiella pneumoniae in a Healthcare Facility

Zhang

Shen

et al. 2022

Antibiotics

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“…The well-diffusion method was used to test the ability of the biosynthesized CNPs to inhibit the growth of multidrug-resistant Acinetobacter baumannii bacteria using swab inoculation assay method. Bacterial suspension was prepared according to the method of Amini Tapouk et al 37 . In sterilized Petri dishes, 50 mL of nutrient agar medium was poured and allowed to solidify.…”

Section: Characterization Of Chitosan Nanoparticlesmentioning

confidence: 99%

Green synthesis of chitosan nanoparticles, optimization, characterization and antibacterial efficacy against multi drug resistant biofilm-forming Acinetobacter baumannii

El‐Naggar

Shiha

Mahrous

et al. 2022

Sci Rep

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Chitosan nanoparticles (CNPs) are promising versatile cationic polymeric nanoparticles, which have received growing interest over last few decades. The biocompatibility, biodegradability, environmental safety and non-toxicity of the chitosan nanoparticles makes it preferred for a wide range of biological applications including agriculture, medical and pharmaceutical fields. In this study, CNPs were biosynthesized by aqueous extract of Eucalyptusglobulus Labill fresh leaves as bio-reductant. Box–Behnken design in 29 experimental runs was used for optimization of different factors affecting the production of CNPs. The maximum yield of CNPs was 9.91 mg/mL at pH of 4.5, chitosan concentration of 1%, incubation time of 60 min and temperature of 50 °C. The crystallinity, particle size and morphology of the biosynthesized CNPs were characterized. The CNPs possess a positively charged surface of 31.1 mV. The SEM images of the CNPs confirms the formation of spherical form with smooth surface. The TEM images show CNPs were spherical in shape and their size range was between 6.92 and 10.10 nm. X-ray diffraction indicates the high degree of CNPs crystallinity. FTIR analysis revealed various functional groups of organic compounds including NH, NH2, C–H, C−O, C–N, O–H, C–C, C–OH and C–O–C. The thermogravimetric analysis results revealed that CNPs are thermally stable. The antibacterial activity of CNPs was determined against pathogenic multidrug-resistant bacteria, Acinetobacterbaumannii. The diameters of the inhibition zones were 12, 16 and 30 mm using the concentrations of 12.5, 25 and 50 mg/mL; respectively. When compared to previous studies, the biosynthesized CNPs produced using an aqueous extract of fresh Eucalyptusglobulus Labill leaves have the smallest particle sizes (with a size range between 6.92 and 10.10 nm). Consequently, it is a promising candidate for a diverse range of medical applications and pharmaceutical industries.

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“…Furthermore, it will reduce the likelihood of bacteria developing resistance to disinfectants in the healthcare setting. The use of disinfectants merely based on the manufacturer's claims is causing increased incidents of microbes getting resistant to disinfectants (Amini et al, 2020). Hence, the results of the present study indicated that it is very important to access the antimicrobial activity of the intended disinfectant before use and that too with standardized methods.…”

Section: Discussionmentioning

confidence: 73%

Bactericidal Activity of Different Generations of Quaternary Ammonium Disinfectants as per EN 1276: A Comparative Study

Sharma¹,

Kiranjeet²,

Kumar³

2022

IJZI

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The most challenging issues facing modern medical practices are microbial infections acquired from Hospitals (HAIs). Different generations of disinfectants based on quaternary ammonium compounds (QACs) are used to address the challenges posed by HAIs. However, the antimicrobial efficacy of disinfectants varies significantly because of compositions and factors like hard water and the presence of interfering materials. The utilization of inefficient QACs disinfectants can result in a further increase in the incidents of HAIs and resistance in microbes. In the present study, the impact of organic load and hard water on the antimicrobial efficacy of different generation QACs disinfectants was evaluated using European norm EN 1276.

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Comparative efficacy of hospital disinfectants against nosocomial infection pathogens

Cited by 32 publications

References 22 publications

Disinfection Strategies for Carbapenem-Resistant Klebsiella pneumoniae in a Healthcare Facility

Disinfection Strategies for Carbapenem-Resistant Klebsiella pneumoniae in a Healthcare Facility

Green synthesis of chitosan nanoparticles, optimization, characterization and antibacterial efficacy against multi drug resistant biofilm-forming Acinetobacter baumannii

Bactericidal Activity of Different Generations of Quaternary Ammonium Disinfectants as per EN 1276: A Comparative Study

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