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Background: Due to the increasing rate of hospital-acquired infections, it is essential to select appropriate disinfectant agents. In this study, the efficacy of hospital disinfectants against nosocomial infection pathogens was compared. Methods: High level disinfectants (Steranios 2%, Deconex HLDPA, and Microzed Quatenol) were tested for their antibacterial effects by determining their minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) against Enterococcus faecalis ATCC 29212 and Burkholderia cepacia ATCC 10673. Results: E. faecalis, as gram-positive bacterium, was more susceptible to high level disinfectants compared to gramnegative B.cepacia. The MIC = MBC values of 2% Steranios, Deconex HLDPA and Microzed Quatenol against E. faecalis and B.cepacia were 0.31, 9.77, 2.2 mg/L and 9.8, 78.13, 70.31 mg/L, respectively. Conclusions: According to the findings of this study, the most effective disinfectants against both E. faecalis and B.cepacia were Steranios 2%, Microzed Quatenol, and Deconex HLDPA in order. Considering the importance of these bacterial strains in healthcare-associated infections, the use of these effective disinfectants is recommended in the hospitals.

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