Comparative evaluation of antibacterial efficacy of Allium sativum extract, aqueous ozone, diode laser, and 3% sodium hypochlorite in root canal disinfection: An in vivo study

Abstract

Aim: The present study aimed to individually evaluate and compare the aerobic and anaerobic antibacterial activity of Allium sativum extract, aqueous ozone, diode laser, and 3% sodium hypochlorite (NaOCl) as root canal irrigants. Materials and Methods: Forty-eight patients were selected and randomly allocated to one of the four groups ( n = 12 each) according to the irrigant to be used in each group. For each included tooth, the preirrigation and postirrigation (after irrigation with the test solution) samples were collected through sterile paper points and microbial culturing was done by swabbing on blood agar plates followed by incubation for aerobic and anaerobic bacteria. Statistical Analysis: Manual colony-forming units counting were done, and statistical analysis was performed. Analysis of variance (one-way) followed by post hoc test was performed as a parametric test to compare the difference between the groups for both aerobic and anaerobic bacteria. Results: All the groups showed a statistically significant reduction in bacteria ( P < 0.05). However, between the groups, the maximum reduction was seen with 3% NaOCl followed by diode laser, A. sativum extract, and least by aqueous ozone. Conclusion: A. sativum extract, aqueous zone, diode laser, and 3% NaOCl showed significant antibacterial activity against aerobic and anaerobic bacteria.

The Effect of High-Power Lasers on Root Canal Disinfection: A Systematic Review

“…26,33,34,36 In contrast, some other studies indicated that the diode laser is effective and acceptable when used solely. 12,19,20 Moreover, when Er, Cr: YSGG is utilized solely, it does not eradicate root canal bacteria effectively; however, using this laser with LAI technique with silver nanoparticles, NaOCl or saline showed acceptable outcomes in disinfecting the root canal system. 21,37,45,50 The Nd: YAG laser has been used only in conjunction with silver nanoparticles and saline.…”

“…14,[16][17][18] However, some studies have reported that merely irradiating the root canal with a diode laser reduces the bacterial load more than traditional irrigation methods. 13,[19][20][21][22][23][24][25] To achieve higher levels of disinfection, the diode laser has been used coincide with irrigation with distilled water, 26,27 ozonated water, 26 saline, 12,22 chlorhexidine, 27 silver nanoparticles, 27 and another laser application. 28 Additionally, the diode laser has been used in the LAI technique with NaOCl, 13,29,30 and most of these studies have reported that LAI is a more potent tactic to disinfect root canals.…”

An innovative technique to safely perform active cleaning in teeth with open apices: CAB technique

“…With the aid of the CAB technique, this powerful technique can be used safely even in cases of teeth with open apices. [ 2 14 22 23 24 25 26 ]…”

Comparative evaluation of the antibacterial effect ofAllium sativum, calcium hydroxide and their combination as intracanal medicaments in infected mature anterior teeth: A randomized clinical trial

“…Therefore, it was interesting to use its extract in root canal disinfection protocols. Previous studies (Elheeny, 2019; Ghoddusi et al, 2021; Mehta et al, 2020) used Allium sativum as an irrigant and showed promising clinical results. However, according to our knowledge, it is not clear whether the AS extracts' usage as an intracanal medicament could give more antibacterial action than the traditionally used calcium hydroxide or not.…”