Comparative evaluation of antimicrobial activity of three cements: new endodontic cement (NEC), mineral trioxide aggregate (MTA) and Portland

Zarrabi, Mohammad Hasan; Javidi, Maryam; Naderinasab, Mahboube; Gharechahi, Maryam

doi:10.2334/josnusd.51.437

Cited by 47 publications

(38 citation statements)

References 31 publications

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“…However, in this study all materials were ineffective against E. faecalis, as similar to those observed in the literature related to Estrela et al 4 , Ribeiro et al 13 , Zarrabi et al 14 and Miyagak et al 24 , in constrast to the results presented in Sipert et al 11 and Tanumaru Filho et al 12 investigations.…”

Section: Discussionsupporting

confidence: 73%

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Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Júnior

Sobral

Sampaio

et al. 2015

RGO, Rev. Gaúch. Odontol.

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ObjectiveThe objective of this study was to evaluate the antimicrobial activity of Mineral trioxide aggregate e Portland cement against some selected cariogenic bacteria. Methods Wells were made of approximately 0.5 mm in diameter, in solid culture media and immediately filled with cement. Twelve samples of each material were obtained for the realization of the agar diffusion method. These samples were tested with Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus (UFV) and Lactobacillus casei (UNICAMP). Petri plates containing Tripticase soy Agar (TSA) were used for the growth of Streptococcus mutans and Enterococcus faecalis, and plates of Man Rogosa & Sharpe Agar (MRS) for the Lactobacillus acidophilus and Lactobacillus casei. After 48 hours of incubation, it was made the measurement of inhibition halos with the aid of manual caliper. ResultsThe diameters obtained to MTA (2.50 ± 0.00;2,92 ± 0.63; 2.58 ± 0.38), PC (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) and calcium hydroxide cement (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) ResultadosOs diâmetros obtidos do MTA (2.50 ± 0.00; 2,92 ± 0.63; 2.58 ± 0.38), CP (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) e cimento de hidróxido de cálcio (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) foram expressos em milímetros e submetidos ao teste de Tukey (p<0.05). Conclusão METHODSThe tested materials were: MTA (Angelus Soluções Odontológicas, Londrina, Brasil), Portland cement (CP II -F32, ITAPESSOCA AGRO-INDUSTRIAL S.A., Goiana, Brasil) and calcium hydroxide paste (Reagen TM).The antimicrobial activity of the materials was evaluated by the Agar diffusion method against four different strains.The strains used for analysis were Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus and Lactobacillus casei isolated by the Laboratory of Microbiologic Science (Federal University of Viçosa -MG (UFV) and University State of Campinas -UNICAMP, SP, Brazil), respectively.Of each bacterial culture tested, it was held a concentration suspension of approximately 5x108 of colonies per mL, corresponding to 0.5 of Mc Farland em range of sterile medium Trypticase Soy Browth (TSBMerck). The Streptococcus mutans and Enterococcus faecalis suspensions were inoculated with spread plate in the middle of Sodium Tioglicolate (Merck) culture and Lactobacillus and Lactobacillus acidophilus casei were inoculated in the culture medium Man Rogosa & Sharpe Agar (MRS-HIMEDIA). Subsequently, the plates were incubated at 35 ± 1°C for 24/48h and the halos of inhibition were expressed in millimeters, using a caliper manual.A total of 12 plates were employed, each microorganism was tested in triplicate. Three cavities, measuring 4mm in diameter were made in each agar plate using a copper puncher and then completely filled with freshly manipulated product to be tested.Positive (CaOH) and negative controls (sterile growing medium) were tested using the same methodology and under the same conditions of the experiment.All cult...

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Section: Discussionsupporting

confidence: 73%

“…The antimicrobial effect of MTA and PC measured the test of inhibition in agar was already observed against microorganisms [13][14][15] . However, no previous studies have evaluated the antimicrobial activity exclusively against bacterial strains of deep decayed dentin [16][17] .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Júnior

Sobral

Sampaio

et al. 2015

RGO, Rev. Gaúch. Odontol.

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“…Considering that rootend filling materials are placed in small cavities (3-mm deep), their properties should ideally include good adhesion to dentine walls and biocompatibility (6). The methodology employed in this study was based on that of previous investigations using the in vitro microbial leakage model (2,4,5). Despite the restraint of leakage study methodology (5), dye and radioisotope leakage models have limitations such as the molecular size of most dye particles being smaller than that of bacteria and these models do not reproduce the interactions between microbial and non-microbial tracers (17).…”

Section: Discussionmentioning

confidence: 99%

“…The ideal properties of root-end filling include optimal apical seal, biocompatibility, adhesiveness to dentine walls and microbial control. Several materials have been recommended for root-end filling, including gutta-percha, amalgam, ZOE cement, Cavit, composite resins, glass ionomers, Super-EBA and mineral trioxide aggregate (MTA) (1)(2)(3)(4)(5). Mineral trioxide aggregate, developed at Loma Linda University, USA, has been indicated for sealing off the pathways of communication between the root canal system and the outer surface of the tooth (1,7).…”

Section: Introductionmentioning

confidence: 99%

Microbial leakage of MTA, Portland cement, Sealapex and zinc oxide-eugenol as root-end filling materials

Estrela

Estrada-Bernabé²,

Decúrcio³

et al. 2011

Med Oral

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Objective: The aim of this study was to compare the microbial leakage of mineral trioxide aggregate (MTA), Portland cement (PC), Sealapex and zinc oxide-eugenol (ZOE) as root-end filling materials. Study design: An in vitro microbial leakage test (MLT) with a split chamber was used in this study. A mixture of facultative bacteria and one yeast (S. aureus + E. faecalis + P. aeruginosa + B. subtilis + C. albicans) was placed in the upper chamber and it could only reach the lower chamber containing Brain Heart Infusion broth by way of leakage through the root-end filling. Microbial leakage was observed daily for 60 days. Sixty maxillary anterior human teeth were randomly assigned to different groups -MTA and PC (gray and white), Sealapex + zinc oxide and ZOE, control groups and subgroups to evaluate the influence of EDTA for smear layer removal. These materials were further evaluated by an agar diffusion test (ADT) to verify their antimicrobial efficacy. Data were analyzed statistically by Kruskal-Wallis and Mann-Whitney test. Results: In the MLT, Sealapex + zinc oxide and ZOE did not show evidence of microbial leakage over the 60-day experimental period. The other materials showed leakage from the 15th day. The presence of smear layer influenced microbial leakage. Microbial inhibition zones were not observed in all samples tested by ADT. Conclusion: Sealapex + zinc oxide and ZOE did not show microbial leakage over the experimental period, whereas it was verified within 15 to 45 days in MTA and Portland cement.

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“…CEM cement has a pH value similar to that of MTA; however, it has higher fluidity as compared to MTA with shorter working time and less film thickness . A study by showed that CEM has antimicrobial activity against pathogens, similar to that of calcium hydroxide and better than that of MTA (Asgary and Kamrani, 2008;Zarrabi et al, 2009). Antifungal effects of MTA and CEM against Candida albicans have been compared and it has been shown that both materials completely destroy the fungus in 24 h…”

Section: Introductionmentioning

confidence: 99%

Effect of adding chlorhexidine to calcium enriched mixture (CEM) on its antimicrobial activity

Froughreyhani¹,

Ghasemi²,

Safaralizadeh³

et al. 2014

Afr. J. Microbiol. Res.

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In recent years, a new endodontic cement, calcium enriched mixture (CEM) has been introduced; with clinical applications similar to those of mineral trioxide aggregate (MTA). It has been shown that CEM has higher antibacterial activity than MTA. On the other hand, use of chlorhexidine (CHX) to promote the antibacterial activity of different dental materials is increasing. The aim of the present study was to evaluate the effect of adding CHX to CEM on its antibacterial activity. The antibacterial activities of the materials under study [(CEM cement + CEM solution + 2%CHX) and (CEM cement + CEM solution)] against Pseudomonas aeroginosa, Enterococcus faecalis, Staphylococcus aureus and Escherichia coli were evaluated using agar diffusion technique, followed by determination of the diameter of microbial zone of inhibition around the materials by three independent observers after 72 h. Data were analyzed by Mann-Whitney U test. Statistical significance was defined at P<0.05. The mean diameters of zones of inhibition in the CEM + CEM liquid and CEM + CEM liquid + CHX groups against P. aeroginosa, E. faecalis, S. aureus and E. coli were (13.2 and 9), (21.10 and 6), (20.2 and 9) and (17 and 9.75) ml, respectively, with larger diameters in the CEM + CEM solution + CHX group as compared to CEM + CEM solution group with all the microorganisms (P<0.05). Incorporation of CHX with CEM resulted in an increase in antimicrobial activity of CEM.

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Comparative evaluation of antimicrobial activity of three cements: new endodontic cement (NEC), mineral trioxide aggregate (MTA) and Portland

Cited by 47 publications

References 31 publications

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Microbial leakage of MTA, Portland cement, Sealapex and zinc oxide-eugenol as root-end filling materials

Effect of adding chlorhexidine to calcium enriched mixture (CEM) on its antimicrobial activity

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