Comparative Evaluation of Antimicrobial Efficacy of Sodium Hypochlorite, MTAD, and Tetraclean Against Enterococcus faecalis Biofilm

Giardino, Luciano; Ambu, Emanuele; Savoldi, E; Rimondini, Roberto; Cassanelli, Clara; Debbia, Eugenio A.

doi:10.1016/j.joen.2007.02.012

Cited by 124 publications

(98 citation statements)

References 29 publications

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“…of colonies were counted. Another method 'Miles and Mishra serial dilution method' which was observed in a study conducted by Giardino et al, [18].…”

mentioning

confidence: 99%

A Comparative Evaluation of the Effectiveness of Three Different Irrigating Solution on Microorganisms in the Root Canal: An Invivo Study

Jain

Yeluri

Garg

et al. 2015

JCDR

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“…of colonies were counted. Another method 'Miles and Mishra serial dilution method' which was observed in a study conducted by Giardino et al, [18].…”

mentioning

confidence: 99%

A Comparative Evaluation of the Effectiveness of Three Different Irrigating Solution on Microorganisms in the Root Canal: An Invivo Study

Jain

Yeluri

Garg

et al. 2015

JCDR

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“…4 Sodium thiosulfate inactivates sodium hypochlorite. 3,4,15,27 In the present study, all the irrigating solutions and associations evaluated promoted elimination of planktonic microorganisms. Abdullah 30 QMiX showed similar results were observed after 5 seconds of contact.…”

Section: 2324mentioning

confidence: 56%

Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

Nascimento

Tanomaru‐Filho

Faria-Júnior

et al. 2014

The Journal of Contemporary Dental Practice

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Aim: To evaluate the antibacterial activity of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) alone or associated with cetrimide (CTR), and QMiX against biofilm and planktonic Enterococcus faecalis (E. faecalis) [American type culture collection (ATCC) 29212]. Materials and methods:The solutions 2.5% NaOCl, 2.5% NaOCl + 0.2% CTR, 2% CHX, 2% CHX + 0.2% CTR, 0.2% CTR, and QMiX were evaluated. E. faecalis biofilms were induced for 14 days on bovine dentin blocks. The irrigants were evaluated after contact with E. faecalis suspension and biofilm for 1 and 3 minutes. After that, serial decimal dilutions were made and plated on tryptic soy agar (TSA) medium. Plates were incubated for 24 hours at 37°C and the colony-forming unit (CFU) 1 ml was determined. Data were subjected to ANOVA and Tukey's tests at 5% significance.Results: All microorganisms were eliminated by direct contact of the irrigants with planktonic cells. Only NaOCl and NaOCl + CTR were able to completely eliminate the microorganisms by direct contact with E. faecalis biofilm. CHX presented effectiveness similar to CHX + CTR CTR, and QMiX after 1 minute of contact and similar to NaOCl and NaOCl + CTR after 3 minutes (p > 0.05), but was unable to completely eliminate the microorganisms. CTR and QMiX did not differ from each other.Conclusion: CTR addition to CHX and NaOCl solutions did not improve the antimicrobial activity against biofilm. All evaluated irrigants and associations presented activity against planktonic E. faecalis. Only NaOCl and NaOCl + CTR eliminated biofilm after 1 and 3 minutes of direct contact.Clinical relevance: Addition of CTR does not modify the antibiofilm effectiveness of CHX and NaOCl.

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“…It can safely remove the smear layer, has antimicrobial property, 8 and has been suggested to be more efficient than sodium hypochlorite in disinfecting the pulp space. [8][9][10] Herbal alternatives have been used in medical and dental science for hundreds of years and have become even more acknowledged today due to their high antibacterial activity, analgesic, biocompatibility, anti-inflammatory, and anti-oxidant properties.…”

Section: Introductionmentioning

confidence: 99%

Comparative antimicrobial efficacy of herbal alternatives ( Emblica officinalis , Psidium guajava ), MTAD, and 2.5% sodium hypochlorite against Enterococcus faecalis : An in vitro study

Dubey¹

2016

Journal of Oral Biology and Craniofacial Research

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Comparative Evaluation of Antimicrobial Efficacy of Sodium Hypochlorite, MTAD, and Tetraclean Against Enterococcus faecalis Biofilm

Cited by 124 publications

References 29 publications

A Comparative Evaluation of the Effectiveness of Three Different Irrigating Solution on Microorganisms in the Root Canal: An Invivo Study

A Comparative Evaluation of the Effectiveness of Three Different Irrigating Solution on Microorganisms in the Root Canal: An Invivo Study

Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

Comparative antimicrobial efficacy of herbal alternatives ( Emblica officinalis , Psidium guajava ), MTAD, and 2.5% sodium hypochlorite against Enterococcus faecalis : An in vitro study

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