Comparative Evaluation of Antioxidant Reactivity between Ovariectomized and Control Urinary Bladder Tissue Using Ferric Reducing Ability of Plasma and Cupric Ion Reducing Antioxidant Capacity Assays

Radu, Florian; Bean, Holly; Schuler, Catherine; Leggett, Robert E.; Levin, Robert M.

doi:10.1111/j.1757-5672.2009.00052.x

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…Absorbance readings were measured in duplicate at 450 nm. The samples of urine and plasma were diluted with water until their readings were within the standard curve and the dilutions and a 1:1 dilution showed a 50% decrease in the absorbance reading 20–22…”

Section: Methodsmentioning

confidence: 99%

A prospective randomized double‐blind trial of grape juice antioxidants in men with lower urinary tract symptoms

Spettel¹,

Chughtai²,

Feustel³

et al. 2012

Neurourology and Urodynamics

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Section: Methodsmentioning

confidence: 99%

A prospective randomized double‐blind trial of grape juice antioxidants in men with lower urinary tract symptoms

Spettel¹,

Chughtai²,

Feustel³

et al. 2012

Neurourology and Urodynamics

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Blood plasma and urinary biomarkers of oxidative stress in cats with urethral obstruction

Quintavalla,

Basini,

Fidanzio

et al. 2024

BMC Vet Res

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Background This study aimed to investigate variations of the oxidative status in cats affected by urethral obstruction (UO) under Feline Idiopathic Cystitis (FIC) and Bacterial Cystitis (BC), in comparison with a group of healthy subjects. In both groups, the levels of several markers (either direct or indirect) indicative of the oxidative attack and of the antioxidant response were analyzed on plasma and urine samples. In particular, the plasma samples were evaluated for nitric oxide (NO), hydroperoxides derived by reactive oxygen activity (d-ROMs test), superoxide anion (O2−), glutathione peroxidase activity (GPx), superoxide dismutase activity (SOD), and ferric reducing antioxidant power (FRAP test); while on urine the levels of NO, d-ROMs, FRAP, SOD, malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) were measured. Urine of UO patients was also subjected to urine-culture test. Results The analytical data on plasma showed that UO, independently of the FIC or BC etiology, induced the insurgence of oxidative stress conditions at the systemic level. In the urine of the UO patients, except for SOD that increased, the markers of redox status were markedly decreased due probably their compromised filtration, thus suggesting involvement of renal function (assessed also by the high levels of plasma creatinine and proteinuria) with no oxidative damage of the lower urinary tract. Moreover, the adoption of a novel oxidative stress index’ (OSI) allowed to establish, by means of a numerical value, the different degrees of oxidative stress conditions for single UO patients, both in terms of oxidative attack and antioxidant response. Conclusions Feline urethral obstruction, induced by Idiopathic Cystitis and Bacterial Cystitis, causes oxidative stress conditions at the systemic level that do not interest the lower urinary tract. Despite to the high variability of the profiles of oxidative stress indexes both in healthy and UO patients, the determination of OSI made possible the evaluation of their single degrees of oxidative stress. Possibly the results of this investigation can be compared with those of correspondent pathologies both in humans and in other animal species.

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Effect of hydrogen peroxide on rabbit urinary bladder citrate synthase activity in the presence and absence of a grape suspension

et al. 2010

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Purpose: The etiology of obstructive bladder dysfunction includes free radical damage to mitochondria. Feeding rabbits a standardized grape suspension protects the ability of the bladder to contract and empty in part by preventing mitochondrial damage, thus maintaining smooth muscle and mucosal metabolism. The objective of the current study is to determine the direct effect of this grape suspension on the response of mitochondria to the oxidative effects of hydrogen peroxide. Materials and Methods: Six male rabbits were anesthetized with sodium pentobarbital and the bladders excised. Four full thickness strips were obtained for contractile studies and the balance separated into smooth muscle and mucosa compartments by blunt dissection. The effect of hydrogen peroxide on the contractile response to field stimulation was quantitated. Each tissue was homogenized and the effects of increasing concentrations of hydrogen peroxide in the presence and absence of grape suspension on citrate synthase activity was determined. Results: Citrate synthase activity was significantly higher in the mucosa than in the muscle. The grape suspension had no effect on control citrate synthase activity. However, the grape suspension provided significant protection of both smooth muscle and mucosal citrate synthase activity. Conclusions: These studies support the conclusion that the grape suspension provides direct protection of mitochondrial function.

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Comparative Evaluation of Antioxidant Reactivity between Ovariectomized and Control Urinary Bladder Tissue Using Ferric Reducing Ability of Plasma and Cupric Ion Reducing Antioxidant Capacity Assays

Cited by 3 publications

References 19 publications

A prospective randomized double‐blind trial of grape juice antioxidants in men with lower urinary tract symptoms

A prospective randomized double‐blind trial of grape juice antioxidants in men with lower urinary tract symptoms

Blood plasma and urinary biomarkers of oxidative stress in cats with urethral obstruction

Effect of hydrogen peroxide on rabbit urinary bladder citrate synthase activity in the presence and absence of a grape suspension

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