Comparative Evaluation of Bactericidal Potential of Four Root Canal Filling Materials against Microflora of Infected Non -Vital Primary Teeth

Abstract

Background and objectives: Since complete debridement of the root canals of the primary teeth is not practically possible due to the highly variable root canal anatomy, success of the endodontic therapy depends partly on the use of antibacterial irrigating agents and root canal filling materials. Recent literature indicates that anaerobes comprise a majority of the bacteria in necrotic root canals of primary teeth. The study determined the antibacterial effectiveness of four root canal filling materials namely Calcium hydroxide,Zinc oxide eugenol, Vitapex and Metapex against microbial specimens obtained directly from necrotic root canals of primary teeth. Method: Microbial specimens were collected using sterile paper points, from 15 primary maxillary and mandibular posterior teeth of randomly selected children in the age group of 4-10 years with infected non vital primary teeth, requiring pulpectomy procedure. The microbial specimens collected were subjected to microbiological analysis and the antimicrobial potential of root canal filling materials were tested using Agar diffusion technique. Results: were statistically analyzed using one-way ANOVA. Facultative/Aerobic organisms were isolated in all the cases, anaerobic organisms were isolated in 80% of the cases, and Candida albicans was isolated in 1 case. ZOE showed superior inhibitory activity against most of the organisms isolated followed by Vitapex, Calcium hydroxide and Metapex in descending order.Conclusion: Our data may be useful as a guide for relative antimicrobial effectiveness or non-effectiveness of the materials employed. In vivo studies are required to state the specific antimicrobial activity and merits and demerits of any of the test filling material.

Antimicrobial Activity of Five Calcium Silicate Based Root Canal Sealers Against a Multispecies Biofilm

“…On the other hand, we compared the antibacterial properties of bioceramic sealers with zinc oxide eugenol cement, in the Sealite group, Candida Albicans count has significantly decreased between day 3 and day 14 (P<0.05) in comparison with the other groups. In fact in a comparative study of Harni Priya et al showed that zinc oxide eugenol had a maximum antifungal activity on Candida Albicans [27], this finding is also in concordance with the comparative study of Saha et al where a zinc oxide eugenol-based sealer showed maximum zone of inhibition affirming the highest antimicrobial activity on Candida Albicans [28]. These results are probably due to the composition and biophysical characteristics of the sealer [19,25,26,27].…”

“…In fact in a comparative study of Harni Priya et al showed that zinc oxide eugenol had a maximum antifungal activity on Candida Albicans [27], this finding is also in concordance with the comparative study of Saha et al where a zinc oxide eugenol-based sealer showed maximum zone of inhibition affirming the highest antimicrobial activity on Candida Albicans [28]. These results are probably due to the composition and biophysical characteristics of the sealer [19,25,26,27].…”

Clinical and radiographic evaluation of pulpectomy in primary teeth: a 18-months clinical randomized controlled trial

“…The bacterial leakage test model of Sisodia R et al indicated that Zinc Oxide Eugenol showed no bacterial leakage and better resistance to bacterial leakage than Apexit plus (a calcium hydroxide based root canal sealer paste) [ 22 ]. ZOE showed better inhibitory activity against most of the organisms isolated than Vitapex, Calcium hydroxide and Metapex, which proved by Harini PM et al [ 23 ]. These characteristics may be the cause of good clinical manifestations of ZOE, and was also the reason why the present study chooses the ZOE as the main component of the mixed paste.…”

Antibacterial Effect of Chitosan and Low-Level Diode Laser Against Enterococcus faecalis in Infected Root Canals in Primary Molars

“…This incomplete bacterial eradication usually occurs because of the presence of accessory canals and other anatomical variations (4) . Therefore, the use of disinfectant agents or systems that poses antibacterial activity was strongly recommended as an effective method that could help the mechanical instrumentation in bacterial eradication (2,5) . Although, the efficacy of these antibacterial agents has controversial results as well as they could have some adverse effects (6) .…”