Comparative evaluation of bioactive phytochemicals in Spinacia oleracea cultivated under greenhouse and open field conditions

Lee, Bum Soo; So, Hae Min; Kim, Sil; Kim, Jung Kyu; Kim, Jin‐Chul; Kang, Dong-Min; Ahn, Mi‐Jeong; Ko, Yoon‐Joo; Kim, Ki Hyun

doi:10.1007/s12272-022-01416-z

Cited by 28 publications

(16 citation statements)

References 40 publications

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“…In line with ongoing research endeavors focusing on the discovery of structurally new compounds and unique bioactive compounds, 7–11 we focused on investigating the presence of new metabolites in the MeOH extract of a plate culture of the fungus P. cornu-damae . In our recent study of P. cornu-damae , we successfully identified and isolated eight macrocyclic trichothecenes, namely miophytocen D, roridin E, roridin F, roridin L-2, satratoxin H, 12′-episatratoxin H, satratoxin I, and trichoverritone, including three previously unreported compounds (roridin F, miophytocen D, and satratoxin I).…”

Section: Introductionmentioning

confidence: 99%

N-Hydroxy-Phe-Phe, a new dipeptide, and cytotoxic macrocyclic trichothecenes from the lethal toxic mushroom Podostroma cornu-damae

Lee,

Jung,

Ryoo

et al. 2023

Org. Biomol. Chem.

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Section: Introductionmentioning

confidence: 99%

N-Hydroxy-Phe-Phe, a new dipeptide, and cytotoxic macrocyclic trichothecenes from the lethal toxic mushroom Podostroma cornu-damae

Lee,

Jung,

Ryoo

et al. 2023

Org. Biomol. Chem.

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“…In this study, bioactivity-guided isolation of the MeOH extract of C. nipponica fruiting bodies and chemical analysis of the active fractions resulted in the isolation of fourteen compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14), including six alkaloids (1-3, 5, 7, and 8), two phenolics (4 and 6), one fatty acid (9), and five steroids (10-14) via LC-MS-based analysis. Among these compounds, compound 1, i.e., N,N-dimethyl-anthranilic acid, exhibited the most significant cytotoxic effect against MDA-MB-231 cells.…”

Section: Discussionmentioning

confidence: 99%

“…In a screening test, the methanol (MeOH) extract of the fruiting bodies of C. nipponica exhibited cytotoxicity toward human breast cancer cells (MDA-MB-231). Subsequent bioactivity-guided isolation of the MeOH extract led to the isolation of fourteen compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14), including six alkaloids (1-3, 5, 7, and 8), two phenolics (4 and 6), one fatty acid (9), and five steroids (10)(11)(12)(13)(14). The isolated compounds were tested for cytotoxicity against MDA-MB-231 cells to identify the antitumor compounds.…”

Section: Introductionmentioning

confidence: 99%

N,N-Dimethyl-anthranilic Acid from Calvatia nipponica Mushroom Fruiting Bodies Induces Apoptotic Effects on MDA-MB-231 Human Breast Cancer Cells

Lee

Jang

et al. 2023

Nutrients

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Breast cancer ranks among the most prevalent malignancies affecting women worldwide, and apoptosis-targeting drugs are attractive candidates for the treatment of cancer. In the current study, we investigated the in vitro cytotoxicity of the mushroom Calvatia nipponica in human breast cancer cells (MDA-MB-231), identified potential antitumor compounds through bioactivity-guided isolation, and elucidated the antitumor, pro-apoptotic molecular mechanisms of the identified bioactive compounds. C. nipponica is edible when young, and it has been used as a food source as well as a traditional medicine in wound dressings. However, only a limited number of studies have reported its chemical composition and biological activities. In the screening test, the methanol extract of C. nipponica fruiting bodies exhibited cytotoxicity against MDA-MB-231 cells. Bioactivity-guided fractionation of the methanol (MeOH) extract and chemical investigation of the active fractions resulted in the isolation of fourteen compounds (1–14), including six alkaloids (1–3, 5, 7, and 8), two phenolic compounds (4 and 6), one fatty acid (9), and five steroids (10–14). The structures of the isolated compounds were determined using NMR spectroscopic methods, liquid chromatography–mass spectrometry, and comparison of data with previously reported values. The isolated compounds (1–14) were tested for cytotoxicity against MDA-MB-231 cells, where compound 1, i.e., N,N-dimethyl-anthranilic acid, exhibited the most significant cytotoxicity against MDA-MB-231 cells, with an IC50 value of 90.28 ± 4.23 μM and apoptotic cell death of 56.01% ± 2.64% at 100 μM. Treatment with compound 1 resulted in an upregulation of protein levels, including cleaved caspase-8, cleaved poly (ADP-ribose) polymerase, Bcl-2-associated X protein (Bax), cleaved caspase-3, cleaved caspase-9, Bad, and Cytochrome c, but decreased the levels of B-cell lymphoma 2 (Bcl-2). Overall, these results indicate that N,N-dimethyl-anthranilic acid (1) may have anti-breast cancer activity and is probably involved in the induction of apoptosis mediated by extrinsic and intrinsic signaling pathways.

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“…In our ongoing effort to advance our exploration of novel and bioactive metabolites derived from intriguing natural sources [ 19 , 20 , 21 , 22 , 23 ], we initiated a study with the aim of discovering potential bioactive compounds from the ethanol (EtOH) extract of S. chaenomeloides leaves, given that the EtOH extract demonstrated antibacterial activity against Helicobacter pylori strain 51. In the present study, the EtOH extract of S. chaenomeloides leaves underwent solvent partitioning, and the solvent-partitioned fractions were subjected to antibacterial activity tests in our bioactivity screening, where we observed that the ethyl acetate (EtOAc) fraction exhibited antibacterial activity against H. pylori strain 51.…”

Section: Introductionmentioning

confidence: 99%

Chaenomelin, a New Phenolic Glycoside, and Anti-Helicobacter pylori Phenolic Compounds from the Leaves of Salix chaenomeloides

Kim,

Kang,

et al. 2024

Plants

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Salix chaenomeloides Kimura, commonly known as pussy willow, is a deciduous shrub and tree belonging to the Salicaceae family. The genus Salix spp. has been known as a healing herb for the treatment of fever, inflammation, and pain relief. The current study aimed to investigate the potential bioactive natural products from S. chaenomeloides leaves and evaluate their antibacterial activity against Helicobacter pylori. A phytochemical investigation of the ethanol (EtOH) extract of S. chaenomeloides leaves led to the isolation of 13 phenolic compounds (1–13) from the ethyl acetate (EtOAc) fraction, which showed antibacterial activity against H. pylori strain 51. The chemical structure of a new phenolic glycoside, chaenomelin (1), was established by a detailed analysis of 1D and 2D (1H-1H correlation spectroscopy (COSY), heteronuclear single-quantum coherence (HSQC), and heteronuclear multiple-bond correlation (HMBC)) nuclear magnetic resonance (NMR), high-resolution electrospray ionization mass spectroscopy (HR-ESIMS), and chemical reactions. The other known compounds were identified as 5-O-trans-p-coumaroyl quinic acid methyl ester (2), tremulacin (3), citrusin C (4), benzyl 3-O-β-d-glucopyranosyl-7-hydroxybenzoate (5), tremuloidin (6), 1-[O-β-d-glucopyranosyl(1→2)-β-d-glucopyranosyl]oxy-2-phenol (7), arbutin cinnamate (8), tremulacinol (9), catechol (10), 4-hydroxybenzaldehyde (11), kaempferol 3-rutinoside (12), and narcissin (13), based on the comparison of their NMR spectra with the reported data and liquid chromatography/mass spectrometry (LC/MS) analysis. The isolated compounds were evaluated for antibacterial activity against H. pylori strain 51. Among the isolates, 1-[O-β-d-glucopyranosyl(1→2)-β-d-glucopyranosyl]oxy-2-phenol (7) and arbutin cinnamate (8) exhibited antibacterial activity against H. pylori strain 51, with inhibitions of 31.4% and 33.9%, respectively, at a final concentration of 100 μM. These results were comparable to that of quercetin (38.4% inhibition), which served as a positive control. Generally, these findings highlight the potential of the active compounds 7 and 8 as antibacterial agents against H. pylori.

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Comparative evaluation of bioactive phytochemicals in Spinacia oleracea cultivated under greenhouse and open field conditions

Cited by 28 publications

References 40 publications

N-Hydroxy-Phe-Phe, a new dipeptide, and cytotoxic macrocyclic trichothecenes from the lethal toxic mushroom Podostroma cornu-damae

N-Hydroxy-Phe-Phe, a new dipeptide, and cytotoxic macrocyclic trichothecenes from the lethal toxic mushroom Podostroma cornu-damae

N,N-Dimethyl-anthranilic Acid from Calvatia nipponica Mushroom Fruiting Bodies Induces Apoptotic Effects on MDA-MB-231 Human Breast Cancer Cells

Chaenomelin, a New Phenolic Glycoside, and Anti-Helicobacter pylori Phenolic Compounds from the Leaves of Salix chaenomeloides

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