Comparative Evaluation of Cytotoxicity of Root Canal Sealers on Cultured Human Periodontal Fibroblasts:<i>In vitro</i>Study

Jagtap, Pritesh; Shetty, Rajesh; Agarwalla, Arpita; Wani, Parag; Bhargava, Karan; Martande, Santosh S.

doi:10.5005/jp-journals-10024-2346

Cited by 9 publications

(2 citation statements)

References 28 publications

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“…When comparing MTT methodology with others, previous studies have demonstrated that MTT prevent over or underestimation of cell viability during cytotoxicity evaluation, thus considered reliable. [34] As well as, MTT has also been used in previous studies to determine cytotoxic effects caused by different dental materials on HGF, [35] human periodontal ligament fibroblast cells, [36] and in mouse fibroblast cells. [37] Likewise, previous studies have evaluated cell viability of TiO 2 N using MTT method on human cells.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of fluoride release in glass ionomer cements modified with titanium dioxide nanoparticles

et al. 2022

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Background: Several efforts have been made to improve the glass ionomer cements (GICs) properties with nanotechnology. Fluoride release in once of most beneficial properties of GICs. The purpose of this study was to evaluate the fluoride release, recharge, and cytotoxicity in GICs reinforced with titanium dioxide nanoparticles (TiO 2 N).Objective: Evaluate the fluoride release, recharge, and cytotoxicity in GICs reinforced with TiO 2 N.Methods: Four GICs, FUJI IX EXTRA (G1c), KETAC MOLAR (G2c), IONOFILL MOLAR (G3c), and FUJI IX (G4c) were combined with TiO 2 N (G1e, G2e, G3e, and G4e) and divided into blocks of 5-mm width and 1-mm thickness 10 each. A total of 80 samples were arranged as follows: GICs alone as negative control (n = 40) and GICs + TiO 2 N as experimental groups (n = 40). The fluoride release was determined for periods of 1, 2, 6, 10, 31, 90, 180, 240, and 300 days. On days 30 and 179, samples were recharged by submerging in 1 mL of 20,000 ppm sodium fluoride gel. Cytotoxic activity was carried out with gingival fibroblasts, using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide cell viability assay.Results: The experimental groups obtained the highest and more constant fluoride released when compared to control groups. After the first recharge, experimental groups (G1e, G3e, and G4e) showed statistically significant results (P = .001, 0.010, and 0.001 respectively) enhancing their recharge ability regarding control groups. The second recharge showed better results in G1e concerning the rest of the groups. No cytotoxic activity was observed in all experimental groups, although significant differences were observed in G3e and G4e regarding control group. Conclusion:The incorporation of TiO 2 N enhance the fluoride release in glass ionomers with a noncytotoxic effect on human gingival fibroblasts.Abbreviations: GICs = glass ionomer cements, G1c = Glass ionomer FUJI IX EXTRA, G2c = Glass ionomer KETAC MOLAR, G3c = Glass ionomer IONOFILL MOLAR, G4c = Glass ionomer IONOFILL MOLAR, G1e = Glass ionomer FUJI IX EXTRA modified with titanium dioxide nanoparticles, G2e = Glass ionomer KETAC MOLAR modified with titanium dioxide nanoparticles, G3e = Glass ionomer IONOFILL MOLAR modified with titanium dioxide nanoparticles, G4e = Glass ionomer IONOFILL MOLAR modified with titanium dioxide nanoparticles,HGF = human gingival fibroblasts, PDL = population doubled level, TiO 2 = titanium dioxide, TISAB = total ionic strength adjustment buffer solution, TiO 2 N = titanium dioxide nanoparticles.

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Section: Discussionmentioning

confidence: 99%

Enhancement of fluoride release in glass ionomer cements modified with titanium dioxide nanoparticles

et al. 2022

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“…For this reason, in vitro cytotoxicity was evaluated using the CCK-8 assay. HPDLFs represent an appropriate model for testing cytotoxicity of root canal medicaments 44 , 45 . Moreover, the normal diploid cells (e.g.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the anti-biofilm effect of poloxamer-based thermoreversible gel of silver nanoparticles as a potential medication for root canal therapy

Liu

Aman

Ainiwaer

et al. 2021

Sci Rep

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The purpose of this study was to design silver nanoparticles (AgNPs) poloxamer thermoreversible gel (AgNPs-PL) and investigate whether this gel could provide sustained antibacterial activity against Enterococcus faecalis (E. faecalis) in the root canal. The gels fabricated were characterized in terms of gelatin temperature, particle size, in-vitro Ag+ release, and elemental content. Cytotoxicity of AgNPs-PL on primary human periodontal ligament fibroblasts (HPDLFs) was examined by CCK-8 assay. Characterization of AgNPs-PL gel revealed that it contained particles existing as large clumps/fused aggregates of different shapes, with a mean diameter of 21.624 ± 14.689 nm, exhibited sustained release of Ag+ for 9 days, and non-toxic to HPDLFs at a low dose (4–32 μg/mL) through 24, 48, and 72 h exposures. The antibacterial effect of 16 and 32 μg/mL concentrations of AgNPs-PL was compared with blank poloxamer gel (PL) and calcium hydroxide (CH) using three methods: (I) agar counting plate, (II) scanning electron microscope (SEM) observations, and (III) confocal laser scanning microscope (CLSM) analysis. AgNPs-PL at the two doses above was more effective than PL and CH in removing E. faecalis biofilm at 1, 3, 9 days. Thus, AgNPs-PL exhibits strong activity against E. faecalis and is easy to produce, with a continuous release profile of Ag+. AgNPs-PL gel may be a candidate for a new root canal disinfection.

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Investigating unset endodontic sealers’ eugenol and hydrocortisone roles in modulating the initial steps of inflammation

et al. 2019

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Introduction. Endodontic treatment success is achieved not only when the cement provides a hermetic seal but also when the injured periapical tissue is regenerated. However, an exaggerated inflammatory reaction hinders tissue regeneration and it has been shown that dental materials affect the inflammatory response through modulation of cytokine secretion. This work was set to investigate a hydrocortisone-containing sealer (Endomethasone N) modulatory effects on the initial steps of inflammation in vitro. Material and methods. Hydrocortisone and eugenol leaching from Endomethasone N and Pulp Canal Sealer (PCS) were quantified by ELISA and spectrofluorometry respectively. The effects of Endomethasone N and Pulp Canal Sealer were studied on Lipopolysaccharides (LPS)-stimulated human periodontal ligament (hPDL) cells. Cytokine (IL-6, TNF-) secretion from cells was quantified by ELISA. Inflammatory cell (THP-1) adhesion to activated endothelial cells, their migration and activation were studied in vitro.Results. Endomethasone N decreased secretion of IL-6 and TNF-α from hPDL cells. THP-1 adhesion to activated endothelial cells (HUVECs) and migration significantly decreased with Endomethasone N while no effect was observed with PCS. Activation of THP-1 decreased with both materials' extracts but was significantly lower with Endomethasone N than with PCS. Conclusion.These results performed in vitro show that Endomethasone N anti-inflammatory effects are due to the presence of hydrocortisone.Clinical Relevance. The presence of hydrocortisone in Endomethasone N is responsible for its local anti-inflammatory effects.

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Comparative Evaluation of Cytotoxicity of Root Canal Sealers on Cultured Human Periodontal Fibroblasts:In vitroStudy

Abstract: Having knowledge of the cytotoxicity of various sealers will help in increasing patient's comfort.

Cited by 9 publications

References 28 publications

Enhancement of fluoride release in glass ionomer cements modified with titanium dioxide nanoparticles

Enhancement of fluoride release in glass ionomer cements modified with titanium dioxide nanoparticles

Evaluation of the anti-biofilm effect of poloxamer-based thermoreversible gel of silver nanoparticles as a potential medication for root canal therapy

Investigating unset endodontic sealers’ eugenol and hydrocortisone roles in modulating the initial steps of inflammation

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