Investigating unset endodontic sealers’ eugenol and hydrocortisone roles in modulating the initial steps of inflammation

Abstract

Introduction. Endodontic treatment success is achieved not only when the cement provides a hermetic seal but also when the injured periapical tissue is regenerated. However, an exaggerated inflammatory reaction hinders tissue regeneration and it has been shown that dental materials affect the inflammatory response through modulation of cytokine secretion. This work was set to investigate a hydrocortisone-containing sealer (Endomethasone N) modulatory effects on the initial steps of inflammation in vitro. Material and methods. Hydrocortisone and eugenol leaching from Endomethasone N and Pulp Canal Sealer (PCS) were quantified by ELISA and spectrofluorometry respectively. The effects of Endomethasone N and Pulp Canal Sealer were studied on Lipopolysaccharides (LPS)-stimulated human periodontal ligament (hPDL) cells. Cytokine (IL-6, TNF-) secretion from cells was quantified by ELISA. Inflammatory cell (THP-1) adhesion to activated endothelial cells, their migration and activation were studied in vitro.Results. Endomethasone N decreased secretion of IL-6 and TNF-α from hPDL cells. THP-1 adhesion to activated endothelial cells (HUVECs) and migration significantly decreased with Endomethasone N while no effect was observed with PCS. Activation of THP-1 decreased with both materials' extracts but was significantly lower with Endomethasone N than with PCS. Conclusion.These results performed in vitro show that Endomethasone N anti-inflammatory effects are due to the presence of hydrocortisone.Clinical Relevance. The presence of hydrocortisone in Endomethasone N is responsible for its local anti-inflammatory effects.

Synthesis, characterization, and evaluation of curcumin‐loaded endodontic reparative material

“…LPS of bacteria in carious lesions stimulate TLRmediated responses, which lead to activation of the proinflammatory The burst release of TNF-α cytokine as a major inflammatory mediator from activated monocytes/macrophages accumulates neutrophils and monocytes in the inflamed tissues. [37] This factor plays a key role in stimulating the inflammatory cascade and activates other cytokines such as IL-1β, IL-6, and IL-8 and growth factors. It has been shown that after bacterial infiltration, this cytokine is synthesized and released by a multitude of cell types and there are well-known correlations between TNF-α levels in the infected pulp tissue and experienced pain by patients.…”

Morpho-functional effects of different universal dental adhesives on human gingival fibroblasts: an in vitro study

“…The major finding in the present study was that all dental adhesives modified inflammatory patterns. Contact with dental materials can, in fact, cause an inflammatory response [43,44] with over-production of inflammatory markers such as IL6, IL-8 [45,46], IL1β, IL-18 [47][48][49], all of which play major roles gingivitis and periodontal destruction [50,51]. Present observations showed all adhesives were associated with increased IL6 and IL8 expression after short-term exposure which dropped sharply at 48 h and increased IL1β at 48 h. We hypothesize this was due to their secretion in the extracellular compartment which might be an interesting starting point for future studies.…”

The flow rate of endodontic sealers in various consistencies

“…Kyung et al found that only bioceramic-based sealer Endosequence BC created smaller diameter of around 18 mm, while others (AH-Plus, AD Seal, Radic-Sealer, EndoSeal MTA, MTA Fillapex, AD Seal i Radic-Sealer) showed satisfying diameters (more than 20 mm) [31]. Jeanneau C et al investigated anti-inflammatory effect of Endomethasone N and Pulp Canal Sealer and found that only standard or thick consistency (adequate saturation of powder with eugenol) had effect on lessening the secretion of interleukine-6 [32].…”

“…Jeanneau C. i sar. ispituju antiinflamatorno dejstvo Endometazona N i silera Pulp Canal i zaključuju da samo standardno zamešan (ili kao gušća konzistencija) ovaj siler omogućava pravilno očvršćavanje i zasićenje cink-oksida eugenolom i na taj način ispoljava i svoje antiinflamatorno dejstvo smanjenjem lučenja interleukina-6 [32].…”