Comparative evaluation of efficacy of Platelet rich fibrin and Hank’s Balanced Salt Solution as a storage medium for an avulsed tooth- an in vitro study

Abstract

To compare the efficacy of platelet-rich fibrin (PRF) and Hank's balanced salt solution (HBSS) in the preservation of the periodontal ligament (PDL) cells viability of avulsed teeth. Methods: A total of 30 non-carious third molars with healthy periodontium, indicated for extraction for orthodontic reasons or chronic pericoronitis, were selected for the study. Samples were divided into four groups: one standard group and one experimental group in addition to two control groups (positive and negative). The positive and negative control group corresponded to immediate and 2-hour dry time respectively. The experimental teeth were bench dried for 40 minutes and then immersed in one of the two storage media: HBSS (standard storage media) and PRF (experimental storage media) for 45 minutes. The teeth in each group were treated with dispase II and collagenase for 30 minutes and later centrifuged for 4 minutes at 1000 rpm. The supernatant was removed with sterile micropipette, the cells were labeled with 0.4% trypan blue, and the number of viable PDL cells was counted with a hemocytometer under a light microscope. One-way Kruskal-Wallis test and Mann-Whitney U test with Boneferroni correction were used for statistical analysis. Results: Results did not demonstrate any statistically significant differences in the viability of PDL cells between the groups with standard and experimental storage media. Group 1 showed a statistically significant difference of mean compared to Groups 2, 3 and 4. When Group 2 was compared with Groups 3 and 4, a P-value>0.05 suggested no statistical significance. Conclusion: Within the parameters of this study, HBSS and PRF demonstrated a similar number of viable PDL cells. Hence, PRF could be a used as a good substitute of HBSS as a storage media for avulsed teeth.

Evaluation of the Effect of Green Tea Extracts on Periodontal Ligament Fibroblasts Viability versus Hank's Balanced Salt Solution

“…In the present analysis, HBSS was used because it is the most studied avulsed tooth storage solution, and several authors have considered it a gold standard solution. HBSS is a pH-balanced salt solution that contains important cell maintenance metabolites and glucose (12) . However, accidents may occur in schools, homes and sporting clubs, where HBSS is not accessible (6) .…”

Severe Case of Delayed Replantation of Avulsed Permanent Central Incisor: A Case Report with Four-Year Follow-Up

“…Nevertheless, the success of this case goes against all expectations given the time the tooth remained dried and out of mouth [ 20 ]. The decision for an extraoral endodontic treatment was based on the lower risk of external infection-related (inflammatory) root resorption and compliance of the patient [ 9 , 21 ]. Splinting was key to maintaining the tooth in a correct position and protects pulpal and periodontal tissues when the tooth suffers soft mobility and function [ 2 , 12 ].…”