Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus

Brocchi, Emiliana; Bergmann, I; Dekker, A.; Paton, David; Sammin, D.; Greiner, Matthias; Grazioli, Santina; Simone, Franco; Yadin, Hagai; Haas, Bernd; Bulut, Nermin; Malirat, Viviana; Neitzert, E.; Goris, Nesya; Parida, Satya; Sørensen, K. J.; Clercq, Kris De

doi:10.1016/j.vaccine.2006.04.050

Cited by 194 publications

(195 citation statements)

References 14 publications

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“…A seropositive result is an indication of previous exposure of cattle to FMDV. The estimated specificity of the test in non-vaccinated and vaccinated cattle = 97-99% (Brocchi et al, 2006;Engel et al, 2008). The estimated sensitivity in non-vaccinated cattle = 97-100% and in vaccinated cattle = 85-86%.…”

Section: Identification Of Fmd Virus Strainsmentioning

confidence: 96%

“…At the NVRI, blood serum samples were processed for detection of FMDV non-structural protein (NSP) antibodies using an ELISA test (3-ABC ELISA; Prionics, Lelystad B. V., The Netherlands) and recommended procedures (Brocchi et al, 2006). The test detects antibodies to the FMD NSP 3-ABC antigens (expressed as a recombinant antigen using a Baculovirus) which are directly coated onto a microplate.…”

Section: Identification Of Fmd Virus Strainsmentioning

confidence: 99%

“…Because the 3-ABC ELISA test used in this study is not perfect (Brocchi et al, 2006;Engel et al, 2008), it is possible that one or more case herds were misclassified. Another limitation was observation (exposure) bias.…”

Section: Case-control Studymentioning

confidence: 99%

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Foot-and-mouth disease virus strains and examination of exposure factors associated with seropositivity of cattle herds in Nigeria during 2007–2009

Fasina

Connell

Talabi

et al. 2013

Preventive Veterinary Medicine

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New outbreaks of foot-and-mouth disease (FMD) occurred in cattle herds in Nigeria during [2007][2008][2009]. The objectives of the study reported here were (i) to identify current FMD virus strains circulating in cattle herds and (ii) to identify exposure factors associated with a seropositive diagnosis of FMD in cattle herds. This study provides evidence that FMD virus serotypes O, A and SAT-2 were co-circulating in cattle herds in Nigeria during 2007-2009. Cattle herds in a neighborhood affected with FMD had higher odds of being classified as seropositive to FMD, compared to herds that were in a neighborhood not affected with FMD (OR = 16.27; 95% CI = 3.61, 18.74; P < 0.01). Cattle herds that share water points along the trek 2 routes with other cattle herds had higher odds of being classified as seropositive to FMD (adjusted OR = 4.15; 95% CI = 0.92, 18.74; P < 0.06). Results from this study can be used by veterinary services in Nigeria and neighboring countries to evaluate current or future FMD control and eradication programs.

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Section: Identification Of Fmd Virus Strainsmentioning

confidence: 96%

Section: Identification Of Fmd Virus Strainsmentioning

confidence: 99%

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Foot-and-mouth disease virus strains and examination of exposure factors associated with seropositivity of cattle herds in Nigeria during 2007–2009

Fasina

Connell

Talabi

et al. 2013

Preventive Veterinary Medicine

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“…The assay was performed as described by Brocchi et al (15). In brief, the test sera and control sera (positive and negative controls) were diluted with diluent buffer at a concentration of 1:100.…”

Section: Detection Of Fmdv Specific Antibodies In Seramentioning

confidence: 99%

Evidence of foot-and-mouth disease virus excretion in the milk ofapparently healthy vaccinated buffaloes in Islamabad, Pakistan

Ahmed¹,

Farooq²,

Zahur³

et al. 2017

Turk J Vet Anim Sci

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The aim of the study was to determine the seroprevalence of foot-and-mouth disease (FMD) and isolation of the FMD virus (FMDV) in the milk of apparently healthy Asian buffaloes. A cross-sectional study was conducted in periurban dairy farms (n = 20) on ≥20 animals per farm in the suburbs of Islamabad, Pakistan. Sera samples (n = 200) were collected to monitor FMDV seroconversions. The analysis of serum samples using ELISA indicated a high seroprevalence of FMD (78%; 156/200) in Asian buffaloes. Milk samples were collected from FMDV seropositive animals only, and virus-specific signals were detected in 12.2% (19/156) of the animals by reverse transcriptase real-time PCR (rRT-PCR). Milk samples positive for FMDV using rRT-PCR were inoculated into LF-BK cells for virus isolation. Two FMDV isolates (serotypes O and Asia-1) were recovered from the milk samples. A high seroprevalence of FMD and isolation of FMDV from the milk of healthy Asian buffaloes may represent a possible role for healthy Asian buffaloes as an FMD reservoir.

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“…3D (27), 2C (20) i 2B (16), jak i polipeptydach, takich jak 3AB (36) oraz 3ABC (9). Wyniki dotychczasowych badań wskazują, że przeciwciała dla białka 3ABC są najbardziej wiarygodnym serologicznym markerem pozwalającym identyfikować i różnicować zwierzęta po przebytej infekcji wirusowej, nosicieli i rekonwalescentów od zwierząt immunizowanych szczepionkami inaktywowanymi (3,5,13,35). Testy diagnostyczne z wykorzystaniem markerów zakażenia mogą być stosowane do badania zarówno zwierząt nieszczepionych, jak i szczepionych, i mają tę przewagę nad konwencjonalnymi metodami serologicznymi, że nie są specyficzne dla określonego serotypu wirusa, co oznacza, że ten sam test może być przydatny do wykrywania przeciwciał dla wszystkich siedmiu serotypów FMDV.…”

Section: Artykuł Przeglądowy Reviewunclassified

DIVA strategy in the eradication of foot-and-mouth disease

Niedbalski¹,

Fitzner²

2017

Medycyna Weterynaryjna

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SummaryFoot-and-mouth disease virus (FMDV), in contrast to the antigen used for the production of FMD vaccine, can replicate in animals. As a result of infection the specific antibody to the viral structural proteins (SPs) and non-structural proteins (NSPs) of FMDV are synthesized. The laboratory diagnostic methods based on individual NSPs, e.g. 3D, 2C and 2B, as well as polypeptides, such as 3AB and 3ABC can be used for differentiation of infected and vaccinated animals (DIVA). This article presents the aim, principle, methods and results of applying DIVA strategy in the eradication of foot-and-mouth disease. The marker vaccines which enable differentiation between infected and vaccinated animals and appropriate ELISA serological tests for detection of antibodies to the NSPs of FMDV have been described. DIVA strategy makes it possible to reduce the economic losses and restore possibilities of international trade in animals and animal products. This strategy may be an alternative of the administrative "stamping-out" eradication method. The essential aim of DIVA strategy is realization of the so-called "vaccinate-to-live" policy, which is based on the principle that vaccinated animals exposed to FMDV will not transmit the virus. These animals are not epidemiologically risky, and therefore do not have to be eliminated. It is necessary to develop new vaccines and improve those already used as well as the application of reliable diagnostic tests to detect FMDV in vaccinated livestock populations.

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Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus

Cited by 194 publications

References 14 publications

Foot-and-mouth disease virus strains and examination of exposure factors associated with seropositivity of cattle herds in Nigeria during 2007–2009

Foot-and-mouth disease virus strains and examination of exposure factors associated with seropositivity of cattle herds in Nigeria during 2007–2009

Evidence of foot-and-mouth disease virus excretion in the milk ofapparently healthy vaccinated buffaloes in Islamabad, Pakistan

DIVA strategy in the eradication of foot-and-mouth disease

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