Comparative evaluation of surface swab and quantitative full thickness wound biopsy culture in burn patients

Uppal, S. K.; Ram, Shobha; Kwatra, Bharti; Garg, Shuchita; Gupta, Rahul

doi:10.1016/j.burns.2006.08.015

Cited by 41 publications

(30 citation statements)

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“…The swabs were plated on blood agar and MacConkey agar. The isolates were identified using conventional identification techniques after incubation for 18-48 hrs at 37 °C [12]. Positive cultures were subcultured on MacConkey agar, as per routine bacteriologic guidelines.…”

Section: Sample Collection and Microbiological Investigationmentioning

confidence: 99%

Occurrence and antimicrobial susceptibility pattern of ESBL-producers among Gram-negative bacteria isolated from burn unit at the Al Shifa hospital in Gaza, Palestine : A short original article

Tayh

Laham²,

Elmanama³

et al. 2015

int. arab. j antimicrob. agents

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Section: Sample Collection and Microbiological Investigationmentioning

confidence: 99%

Occurrence and antimicrobial susceptibility pattern of ESBL-producers among Gram-negative bacteria isolated from burn unit at the Al Shifa hospital in Gaza, Palestine : A short original article

Tayh

Laham²,

Elmanama³

et al. 2015

int. arab. j antimicrob. agents

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“…(Arévalo G. Comunicación personal). Si bien es cierto, la técnica "patrón de oro" para identifi car y cuantifi car los microorganismos involucrados en la infección de las quemaduras es el cultivo cuantitativo de la biopsia de tejido, dicha técnica no estaría justifi cada en el caso de quemaduras infectadas de manejo ambulatorio, especialmente tratándose de niños, por lo cruenta que resulta la obtención de la muestra y porque existen varios estudios que evidencian una buena correlación entre el cultivo cuantitativo de biopsia y el cultivo cuantitativo y semicuantitativo de muestras obtenidas mediante el hisopado con tórula de la superfi cie quemada 3 . La colonización bacteriana que precede a la infección de las quemaduras de manejo ambulatorio ha sido escasamente estudiada, siendo el objetivo del presente estudio identifi car y cuantifi car las bacterias que colonizan las quemaduras no infectadas y compararlo con las que colonizan la piel sana de localización homóloga en niños que reciben manejo ambulatorio.…”

Section: Hospital Clínico Mutual Deunclassified

Colonización bacteriana de piel sana versus quemada de niños bajo 15 años tratados en COANIQUEM, Santiago de Chile

F³

et al. 2012

Rev. chil. infectol.

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Bacterial colonization in areas of burnt skin and healthy children younger than 15 years, seen COANIQUEM, Santiago, ChileBacterial colonization in patients with burns skin without infection and outpatient management has been poorly studied. Objective: To quantify and identify the type of bacteria that colonize healthy body areas homologous location of the skin in children from 1 to 15 years, seen COANIQUEM, Santiago, Chile. Per patient, we studied 204 samples from each zone. The isolated microorganisms were S. epidermidis 35, 3%; others Staphylococcus coagulase negative, 25.8%; S. aureus, 4.4%; and S. viridans group 1.4%. In the subgroup of children who had a count between 1 and 1000 cfu, there was a higher proportion of cfu in burned skin healthy skin (p = 0.0359). The association between depth of the lesion and the bacterial count obtained p = 0.034. Conclusion: In outpatient treatment, the microorganisms are expected in healthy skin, burned skin is evident in lower counts probably associated with epithelial damage.

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“…Similarly, Danilla et al [6] found that the global concordance between the two methods was moderate (52%); however, they also noted that only S. aureus and A. baumanii showed greater than 60% concordance. Uppal et al [5] reported that both sampling methods yielded the same organisms in 95% of cases; in their study, biopsy was found to be a more valuable method because it demonstrates the critical load of an organism beyond which metastatic invasion of the organism takes place. Basak et al [7] noted a 72 percent correlation between surface swab culture and quantitative wound biopsy culture results.…”

Section: Escherichia Coli 0%mentioning

confidence: 99%

“…The tissue was then weighed, suspended in 2 ml of physiological saline and homogenized. After making serial dilutions, 0.1 ml of undiluted or diluted sample was inoculated on blood and MacConkey Agar [5,7]. Surface viable counts were determined, and the number of organisms per gram of tissue was calculated.…”

Section: Methodsmentioning

confidence: 99%

“…Surface cultures are useful for identifying the organisms present within the burn wound and for identifying the predominant members of the burn wound flora, but these samples are incapable of differentiating burn wound colonization from burn wound infection [5]. It is now accepted that quantitative cultures of biopsy samples from burn wounds, histopathological examination of burn wound specimens and clinical signs and symptoms of infection are required to differentiate between colonization and actual infection.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Surface Swab and Quantitative Biopsy Cultures Dependent on Isolated Microorganisms from Burn Wounds

Vural

Altoparlak

Çelebi

et al. 2013

EAJM

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Objective: Infections are one of the most important and potentially serious complications of burn wounds. Quantitative biopsy cultures are useful for showing that a burn wound infection is not present, but these cultures are not useful for showing that an infection is present. Swab cultures are used to diagnosis surface colonization and may not accurately reflect the organism(s) causing the wound infection. Materials and Methods:To perform a comparison of surface swab and biopsy cultures for isolated microorganisms from burn wounds, 160 biopsy/surface swab pairs were collected from 160 patients.Results: Seventy-seven patients (48.1%) showed positive microorganism cultures from the burn wounds; 19.3 percent had both positive surface cultures and significant bacterial counts from the biopsy cultures, whereas 28.8 percent of the patients had only positive surface cultures. There was moderate agreement (41%) between the results of surface swab and quantitative biopsy cultures used to diagnose burn wound infections. Pseudomonas aeruginosa (45.5%) was the most predominantly isolated bacteria from the wounds, and there was good concordance between the results of the surface swab and quantitative biopsy culture samples (78%). Coagulase-negative Staphylococci (CNS) and Escherichia coli strains were only isolated from the surface swab samples because they are colonizing bacteria. The univariate analysis revealed that there were significant associations between the results of the positive biopsy cultures and the total surface body area, open flame burns, prolonged hospitalization and female gender (p<0.05). Conclusion:Surface swab and quantitative biopsy cultures have a high rate of concordance in predicting P. aeruginosa invasion and the colonization of E. coli and CNS strains in burn wounds.Key Words: Burn wound, Surface swab culture, Quantitative biopsy culture Özet Amaç: Yanık yarası enfeksiyonları; yanığın çok önemli ve ciddi komplikasyonlarından birisidir. Kantitatif biyopsi kültürleri yanık alanında bir yara enfeksiyonu bulunmadığını desteklemek için yararlıdır, ancak bir enfeksiyon olup olmadığını göstermek için kullanışlı değildir. Sürüntü kültürleri yüzeyel kolonizasyonun tanısı amacıyla kullanılır ve gerçekten yara enfeksiyonuna neden mikroorganizma(ları) yansıt-mayabilir. Gereç ve Yöntem:Yanık yarasından izole edilen mikroorganizmalar baz alınarak yüzeyel sürüntü ve kantitatif biyopsi kültür sonuçlarını karşılaştırmak amacıyla, 160 hastadan eş zamanlı olarak sürüntü ve biyopsi örnekleri alındı. Bulgular: Yetmişyedi (%48.1) hastanın yanık yaralarından mikroorganizma izole edildi. Bu hastaların %19.3'ünün hem biyopsi kültürle-rinde anlamlı sayılarda mikroorganizma, hem de sürüntü kültürü pozitifliği saptanırken, %28.8'inde sadece sürüntü örneği kültürlerinde üreme tespit edildi. Yanık yarasının mikroorganizmalarca invazyonunun gösterilmesinde, yüzeyel sürüntü ve kantitatif biyopsi kültürleri arasında ki uyum orta derecede idi (%41). Pseudomonas aeruginosa en sık izole edilen mikroorganizma olup (%45.5), b...

show abstract

Comparative evaluation of surface swab and quantitative full thickness wound biopsy culture in burn patients

Cited by 41 publications

References 9 publications

Occurrence and antimicrobial susceptibility pattern of ESBL-producers among Gram-negative bacteria isolated from burn unit at the Al Shifa hospital in Gaza, Palestine : A short original article

Occurrence and antimicrobial susceptibility pattern of ESBL-producers among Gram-negative bacteria isolated from burn unit at the Al Shifa hospital in Gaza, Palestine : A short original article

Colonización bacteriana de piel sana versus quemada de niños bajo 15 años tratados en COANIQUEM, Santiago de Chile

Comparison of Surface Swab and Quantitative Biopsy Cultures Dependent on Isolated Microorganisms from Burn Wounds

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