Comparative evaluation of the CD4+CD8+ and CD4+CD8− lymphocytes in the immune response to porcine rubulavirus

Hernández, Jesús; Garfias, Yonathan; Nieto, Alejandro; Mercado, Carmen M.; Montaño, Luis F.; Zenteno, Edgar

doi:10.1016/s0165-2427(01)00259-8

Cited by 30 publications

(15 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies have revealed that DP cells are also widely distributed outside of the thymus and belong to an independent subset of lymphocytes [29][30][31][32]. DP cells have the function of CD4 + cells and CD8 + cells [33,34]. In our study, LLE treatment significantly up-regulated the populations of CD4 + CD8 -cells and CD4 + CD8 + cells (DP cells).…”

Section: Discussionsupporting

confidence: 60%

In vitro immunomodulatory effects of an oleanolic acid-enriched extract of Ligustrum lucidum fruit (Ligustrum lucidum supercritical CO2 extract) on piglet immunocytes

Wang

Shan

Liu

et al. 2012

International Immunopharmacology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 60%

In vitro immunomodulatory effects of an oleanolic acid-enriched extract of Ligustrum lucidum fruit (Ligustrum lucidum supercritical CO2 extract) on piglet immunocytes

Wang

Shan

Liu

et al. 2012

International Immunopharmacology

View full text Add to dashboard Cite

“…[1][2][3][4][5][6] The existence of this unconventional and rare (Ͻ 5%) lymphocyte population in the peripheral blood was explained as a premature release of CD4 ϩ CD8 ϩ T cells from the thymus into the periphery, 7,8 where their maturation into functionally competent single-positive cells continues. 7 There is, however, considerable evidence of an increased frequency of peripheral CD4 ϩ CD8 ϩ T cells during viral infections.…”

Section: Introductionmentioning

confidence: 99%

Peripheral CD4+CD8+ T cells are differentiated effector memory cells with antiviral functions

Nascimbeni

Shin

Chiriboga

et al. 2004

Blood

227

198

View full text Add to dashboard Cite

IntroductionT-cell development is an ordered process thought to take place exclusively in the thymus where CD4 ϩ CD8 ϩ T cells develop into CD4 ϩ and CD8 ϩ T cells with mutually exclusive expression of these 2 receptors. Mature CD4 ϩ and CD8 ϩ T cells then leave the thymus and enter secondary lymphoid organs where they recognize their cognate antigen in the context of major histocompatibility complex (MHC) class II and class I molecules on antigenpresenting cells. Antigenic stimulation induces proliferation and differentiation into armed effector cells with the ability to home to infected organs and to participate in immune responses against intracellular pathogens.Contrary to this conventional dichotomy, circulating CD4 ϩ CD8 ϩ T cells have sporadically been reported in humans as well as in animals such as mice, chicken, swine, and monkeys. [1][2][3][4][5][6] The existence of this unconventional and rare (Ͻ 5%) lymphocyte population in the peripheral blood was explained as a premature release of CD4 ϩ CD8 ϩ T cells from the thymus into the periphery, 7,8 where their maturation into functionally competent single-positive cells continues. 7 There is, however, considerable evidence of an increased frequency of peripheral CD4 ϩ CD8 ϩ T cells during viral infections. [9][10][11][12] In human immunodeficiency virus (HIV) or EpsteinBarr virus (EBV) infections, for example, the percentage of CD4 ϩ CD8 ϩ T cells can increase to 20% of all circulating lymphocytes. 9,10 Yet, in humans, very little is known about their phenotype, antigen specificity, and function. This is particularly striking since in animal models CD4 ϩ CD8 ϩ T cells have been described as antigen-specific memory cells that can be induced by vaccination. 3,4,6 Thus, it is crucial to determine the role and biologic significance of peripheral CD4 ϩ CD8 ϩ T cells in humans, as they could potentially contribute to the adaptive immune response against pathogens.In this study, we addressed this issue with an extensive analysis of peripheral CD4 ϩ CD8 ϩ T cells in both healthy individuals and patients with past or present viral infections. We determined the frequency and ex vivo phenotype of CD4 ϩ CD8 ϩ T cells in regards to memory, activation, homing, differentiation, and maturation markers. Furthermore, we provide data on antigen specificity, proliferation, cytokine production, and cytotoxic potential of CD4 ϩ CD8 ϩ T cells in response to tetanus toxoid and to a large variety of antigens (lysates of infected cells, overlapping viral peptides, minimal optimal viral epitopes) from past (influenza A virus [IV] Materials and methods Blood samplesPeripheral blood mononuclear cells (PBMCs) were isolated from anticoagulant citrate dextrose (ACD)-anticoagulated blood of 10 healthy blood donors and 15 HCV-infected patients as described. 13 Viral lysates, peptides, proteins, and antibodiesLysates from CMV-or EBV-infected cells and the corresponding uninfected cells were purchased from Virion (Marristown, NJ). Lysates from HSV-1-, MV-, and VZV-infected cells and the c...

show abstract

“…Escherichia coli O55:B5 lipopolysaccharide (LPS) and PCR primers and probe were from Sigma (St. Louis, MO). Blood samples were taken from 4-to 8-week-old White/Yorkshire hybrid pigs, and peripheral blood mononuclear cells (PBMCs) were isolated in gradients of FicollHypaque as previously described (8) and resuspended in complete Dulbecco's modified Eagle's medium (DMEM) containing 10% heat-inactivated fetal bovine serum, 50 mM 2-mercaptoethanol, 100 IU penicillin/ml, and 100 g streptomycin/ml. PBMCs were placed in 75-cm 2 tissue culture flasks, and monocytes were allowed to adhere through overnight incubation at 37°C in 5% CO 2 .…”

mentioning

confidence: 99%

“…The expression of CD14, CD172a, MHC-II, CD80/86, and PRRSV N protein (determined by intracellular staining) was evaluated 24 h postinfection (hpi) by flow cytometry as previously described (8). At 24 hpi, RNA was extracted from infected and noninfected mDCs using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions.…”

mentioning

confidence: 99%

Porcine Reproductive and Respiratory Syndrome Virus Infects Mature Porcine Dendritic Cells and Up-Regulates Interleukin-10 Production

Flores-Mendoza

Silva‐Campa

Reséndiz

et al. 2008

Clin Vaccine Immunol

104

View full text Add to dashboard Cite

Porcine reproductive and respiratory syndrome virus (PRRSV) infects mature dendritic cells (mDCs)derived from porcine monocytes and matured with lipopolysaccharide. The infection of mDCs induced apoptosis, reduced the expression of CD80/86 and major histocompatibility complex class II molecules, and increased the expression of interleukin-10, thus suggesting that such mDC modulation results in the impairment of T-cell activation.Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped RNA virus that belongs to the Arteriviridae family (15). PRRSV is responsible for significant economic losses and is the cause of the most important infectious disease affecting swine production worldwide. PRRSV infects pigs of different ages, and cells supporting PRRSV replication are located in different organs and tissues, with alveolar macrophages being the principal cell type that supports replication. Apoptotic cells have been associated with PRRSV infection, and they are distributed in different infected tissues, including lung, testes, and lymph node (21,22). Protective immunity against PRRSV is not clearly understood. A delay in the appearance of the cellular immune response suggests that PRRSV infection involves a mechanism of immunosuppression or immunomodulation (18). Recently, it was reported that PRRSV infects and interferes with some functions of immature monocyte-derived dendritic cells (DCs) (3,11,27), as evidenced by the down-regulation of alpha interferon (IFN-␣) mRNA and the up-regulation of IFN-␤ and tumor necrosis factor alpha (TNF-␣) mRNA without changes in the production of interleukin-10 (IL-10), . No report exists, however, on the effects of PRRSV on mature DCs (mDCs). In this paper, we report the effects of PRRSV on mature porcine monocyte-derived DCs. Our results show that PRRSV replicated in mDCs, induced apoptosis, down-regulated the expression of CD80/86 costimulatory and major histocompatibility complex class II (MHC-II) molecules, reduced the allogeneic stimulation of T cells, and up-regulated the expression of IL-10 (mRNA and protein).The PRRSV strain NVSL 97-7895 (GenBank accession no. AY545985) was propagated in MARC-145 cells, and the supernatant was collected, titrated, and stored at Ϫ70°C. Porcine alveolar macrophages (PAM) were collected and maintained as described previously (14). The following monoclonal antibodies were used: for MHC-II, MSA3; for CD172a, SWC3(74-22-15); and for CD14, CAM36A; all were from VMRD (Pullman, WA). Other key reagents included mouse immunoglobulin G (IgG) Fc-human cytotoxic T-lymphocyte-associated antigen 4 fusion protein (501-820; Ancell, Bayport, MN), goat anti-mouse IgG-fluorescein isothiocyanate (IgG-FITC) (Southern Biotech), and recombinant porcine IL-4 (PSC0045) and recombinant porcine granulocyte-macrophage colony-stimulating factor (PSC2015) from Biosource International (Camarillo, CA). Escherichia coli O55:B5 lipopolysaccharide (LPS) and PCR primers and probe were from Sigma (St. Louis, MO). Blood samples were taken from 4-to 8-week-old...

show abstract

Comparative evaluation of the CD4+CD8+ and CD4+CD8− lymphocytes in the immune response to porcine rubulavirus

Cited by 30 publications

References 32 publications

In vitro immunomodulatory effects of an oleanolic acid-enriched extract of Ligustrum lucidum fruit (Ligustrum lucidum supercritical CO2 extract) on piglet immunocytes

In vitro immunomodulatory effects of an oleanolic acid-enriched extract of Ligustrum lucidum fruit (Ligustrum lucidum supercritical CO2 extract) on piglet immunocytes

Peripheral CD4+CD8+ T cells are differentiated effector memory cells with antiviral functions

Porcine Reproductive and Respiratory Syndrome Virus Infects Mature Porcine Dendritic Cells and Up-Regulates Interleukin-10 Production

Contact Info

Product

Resources

About