Comparative expression analysis of pfdn6a and tcp1? during Xenopus development

Marracci, Silvia; Martini, Davide; Giannaccini, Martina; Giudetti, Guido; Dente, Luciana; Andreazzoli, Massimiliano

doi:10.1387/ijdb.140275ma

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…One well-known member of Group II chaperonin is tailless complex polypeptide 1 ring complex (TRiC)/chaperonin containing tailless complex polypeptide 1 (CCT). TRiC/CCT localizes to both the nucleus and cytosol ( Marracci et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Prefoldin Function in Cellular Protein Homeostasis and Human Diseases

Tahmaz

Ghahe

Topf

2022

Front. Cell Dev. Biol.

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Cellular functions are largely performed by proteins. Defects in the production, folding, or removal of proteins from the cell lead to perturbations in cellular functions that can result in pathological conditions for the organism. In cells, molecular chaperones are part of a network of surveillance mechanisms that maintains a functional proteome. Chaperones are involved in the folding of newly synthesized polypeptides and assist in refolding misfolded proteins and guiding proteins for degradation. The present review focuses on the molecular co-chaperone prefoldin. Its canonical function in eukaryotes involves the transfer of newly synthesized polypeptides of cytoskeletal proteins to the tailless complex polypeptide 1 ring complex (TRiC/CCT) chaperonin which assists folding of the polypeptide chain in an energy-dependent manner. The canonical function of prefoldin is well established, but recent research suggests its broader function in the maintenance of protein homeostasis under physiological and pathological conditions. Interestingly, non-canonical functions were identified for the prefoldin complex and also for its individual subunits. We discuss the latest findings on the prefoldin complex and its subunits in the regulation of transcription and proteasome-dependent protein degradation and its role in neurological diseases, cancer, viral infections and rare anomalies.

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Section: Introductionmentioning

confidence: 99%

Prefoldin Function in Cellular Protein Homeostasis and Human Diseases

Tahmaz

Ghahe

Topf

2022

Front. Cell Dev. Biol.

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“…Xenopus embryos were generated and staged as previously described (Marracci et al, 2015). Whole-mount in situ hybridization was performed as previously described (Watanabe et al, 2002).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

“…Scale bars in (A,B,C,D,E,F,G,H) 200 mm; in (A', E') 50 mm. 316 D. Martini et al described (D'Autilia et al, 2010, Marracci et al, 2015.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Comparative analysis of p4ha1 and p4ha2 expression during Xenopus laevis development

Martini

Giannaccini²,

Guadagni³

et al. 2019

Int. J. Dev. Biol.

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Collagen prolyl 4-hydroxylases (c-P4Hs) are evolutionary conserved enzymes whose activity is essential for the correct folding of stable triple helical molecules of collagen and collagen-like proteins. They play crucial roles in embryo development, connective tissue functional organization, tumor growth and metastasis. Despite the important function of these enzymes, little is known about their expression during vertebrate development. In this study, we determine and compare the previously undescribed spatio-temporal expression patterns of the p4ha1 and p4ha2 genes, which encode the main subunits containing the enzyme active site, during Xenopus development. The two genes are maternally inherited and share expression in dorsal mesoderm, branchial arches and their derivatives, as well as in the central nervous system, although with distinct spatio-temporal patterns. A major co-expression domain for p4ha1 and p4ha2 is represented by the developing notochord, where these genes are transcribed from early neurula stage to stage 42 tadpole, thus paralleling the profile of collagen II production and suggesting a coordination between collagen synthesis and its post-translational modifications.

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“…After Hoechst-counterstaining, the sections were washed and mounted in Aqua Poly/Mount (Polysciences Inc.) (see Marracci et al, 2015).…”

Section: Paraffin Sectioningmentioning

confidence: 99%

pdzrn3 is required for pronephros morphogenesis in Xenopus laevis

Marracci¹,

Vangelisti²,

Raffa³

et al. 2016

Int. J. Dev. Biol.

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Pdzrn3, a multidomain protein with E3-ubiquitin ligase activity, has been reported to play a role in myoblast and osteoblast differentiation and, more recently, in neuronal and endothelial cell development. The expression of the pdzrn3 gene is developmentally regulated in various vertebrate tissues, including muscular, neural and vascular system. Little is known about its expression during kidney development, although genetic polymorphisms and alterations around the human pdzrn3 chromosomal region have been found to be associated with renal cell carcinomas and other kidney diseases. We investigated the pdzrn3 spatio-temporal expression pattern in Xenopus laevis embryos by in situ hybridization. We focused our study on the development of the pronephros, which is the embryonic amphibian kidney, functionally similar to the most primitive nephric structures of human kidney. To explore the role of pdzrn3 during renal morphogenesis, we performed loss-of-function experiments, through antisense morpholino injections and analysed the morphants using specific pronephric markers. Dynamic pdzrn3 expression was observed in embryonic tissues, such as somites, brain, eye, blood islands, heart, liver and pronephros. Loss of function experiments resulted in specific alterations of pronephros development. In particular, at early stages, pdzrn3 depletion was associated with a reduction of the pronephros anlagen and later, with perturbations of the tubulogenesis, including deformation of the proximal tubules. Rescue experiments, in which mRNA of the zebrafish pdzrn3 orthologue was injected together with the morpholino, allowed recovery of the kidney phenotypes. These results underline the importance of pdzrn3 expression for correct nephrogenesis.

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Comparative expression analysis of pfdn6a and tcp1? during Xenopus development

Cited by 4 publications

References 23 publications

Prefoldin Function in Cellular Protein Homeostasis and Human Diseases

Prefoldin Function in Cellular Protein Homeostasis and Human Diseases

Comparative analysis of p4ha1 and p4ha2 expression during Xenopus laevis development

pdzrn3 is required for pronephros morphogenesis in Xenopus laevis

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