Comparative faecal microbiota of dogs with and without calcium oxalate stones

Abstract

Aims The absence of enteric oxalate‐metabolizing bacterial species (OMBS) increases the likelihood of calcium oxalate (CaOx) urolithiasis in humans and dogs. The goal of this study was to compare the gut microbiota of healthy dogs and CaOx stone formed dogs (CaOx‐dogs), especially with respect to OMBS. Methods and Results Faecal samples from healthy and CaOx‐dogs were obtained to analyse the hindgut microbiota by sequencing the V3 region of bacterial 16S rDNA. In total, 1223 operational taxonomic units (OTUs) were identified at 97% identity. Only 38% of these OTUs were shared by both groups. Significant differences in the relative abundance of 152 OTUs and 36 genera were observed between the two groups of dogs. Conclusions The faecal microbiota of healthy dogs is distinct from that of CaOx‐dogs, indicating that the microbiota is altered in CaOx‐dogs. Significance and Impact of the Study This is the first study that has compared the gut microbial diversity in healthy and CaOx‐dogs. Results of this study indicate the future need for functional and comparative analyses of the total array of oxalate‐metabolizing genes between healthy and CaOx stone formers, rather than focusing on specific bacterial species, to understand the critical role of OMBS in CaOx urolithiasis.

Hyperoxaluria leads to dysbiosis and drives selective enrichment of oxalate metabolizing bacterial species in recurrent kidney stone endures

“…Uremic toxin including oxalate may alter the gut biochemical milieu, which may consequently affect structure and composition of gut microbial communities 3 . Previous observations confirm altered microflora with respect to chronic kidney disease 21 22 , but specific reports on alteration in gut microbiome associated with oxalate stones in human subjects are scanty 23 . In the present study, we reveal differences in bacterial community structure in 24 male subjects suffering from idiopathic hyperoxaluria and show enrichment of oxalate metabolizing microbes in their gut.…”

The Gastrointestinal Tract of the White-Throated Woodrat (Neotoma albigula) Harbors Distinct Consortia of Oxalate-Degrading Bacteria

“…Our study is the first to show that E. gallinarum is also capable of degrading oxalate. Similarly, while members of the Clostridium genus exhibit oxalate-degrading activity (49,50,53), this is the first report of oxalate degradation in C. sporogenes. It is not known if these genera use the same pathway as Oxalobacter, Lactobacillus, Bifidobacterium, and others.…”

Association of Obesity with Serum Leptin, Adiponectin, and Serotonin and Gut Microflora in Beagle Dogs

“…One time comparison has been used to evaluate bacterial differences between two different treatment groups or diseased animals in various studies because gut microbiome of healthy adult individual would have similar gut microbiome. 18,47,48 As such, we compared microbiome populations between groups at one time point which is 6 month after the obesity induction. This might weaken our conclusions addressing associations between microbiome shifts and obesity.…”