Comparative FISH-mapping of six expressed gene loci to river buffalo and sheep chromosomes

Abstract

Six expressed gene loci (NF1, CRYB1, CHRNB1, TP53, P4HB and GH1), recently assigned to cattle chromosome 19 by both radiation hybrid analysis and FISH-mapping, were comparatively FISH-mapped to river buffalo chromosome (BBU) 3p and sheep chromosome (OAR) 11, extending the physical map in these two important bovids. The six loci mapped to the same homoeologous chromosome bands of BBU 3p and OAR 11, and their gene order was centromere–NF1–CRYB1–CHNRB1–TP53–(GH1, P4HB).

Comparative FISH mapping of 32 loci reveals new homologous regions between donkey and horse karyotypes

“…Large insert clones have earlier been successfully used across closely related species belonging to the same mammalian order or family (e. g. across bovids - Prakash et al, 1997;Iannuzzi et al, 1999;bovids and suids -Pinton et al, 2000;equids -Raudsepp et al, 1999). However, as compared to this, hybridization of genomic clones across species belonging to different mammalian orders is relatively less common (Frengen et al, 1997;Dixkens et al, 1998;Godard et al, 2000).…”

Comparative FISH mapping in river buffalo and sheep chromosomes: assignment of forty autosomal type I loci from sixteen human chromosomes

“…Both problems can be easily solved by comparative FISH mapping of type I loci which are highly conservative among mammalian genomes. Figure 2 shows some comparisons we made between bovid (sheep and river buffalo) and human chromosome physical maps with type I loci mapped on specific chromosome regions in the present and previous studies (Iannuzzi, 1998;Iannuzzi et al, 1999aIannuzzi et al, , 1999bIannuzzi et al, , 2000aDi Meo et al, 2000;SheepBase) CAD and ASS (OAR3p and BBU12) map on two different human chromosomes (HSA2p22 and HSA9q34, respectively). Furthermore, considering that IL1B and PAX8 map in the central and telomeric region of OAR3p and on the same chromosome region in HSA2q12 → q14, at least four rearrangements occurred between OAR3p (and homoeologous BBU12) and HSA2/HSA9q.…”

“…These homoeologies have been confirmed by both comparative FISH mapping (Hayes et al, 1993;Iannuzzi et al, 1993Iannuzzi et al, , 1999aIannuzzi et al, , 2000aLopez-Corrales et al, 1998) and linkage mapping studies (Crawford et al, 1995;de Gortari et al, 1998). By using linkage mapping analysis alone, Crawford et al, (1995) andde Gortari et al (1998) found a simple chromosome segment translocation occurring between cattle chromosome 9 and sheep chromosome 9 (homoeologous to BTA14/CHI14).…”

“…The physical map on OAR11 and BBU3p was enriched by four more loci (LPO, PNMT, GAS and MAP2), in addition to those FISH mapped earlier (Iannuzzi et al, 1999a;SheepBase). Until now, chromosome band BBU3p22 has been the only one of this chromosome arm without mapped type I loci.…”

Comparative mapping of twenty-eight bovine loci in sheep (<i>Ovis aries</i>, 2n = 54) and river buffalo (<i>Bubalus bubalis</i>, 2n = 50) by FISH

“…River buffalo and sheep represent two important bovids but their physical maps are relatively poor, especially that of river buffalo with a small number of markers assigned, mainly by FISH (Iannuzzi 1998;Iannuzzi et al 1999Iannuzzi et al , 2000aIannuzzi et al , b, 2001aDi Meo et al, 2000).…”