Comparative Gene Expression Analysis of the Coronal Pulp and Apical Pulp Complex in Human Immature Teeth

Kim, Soo Hyun; Kim, Seunghye; Shin, Young Goo; Lee, Hyo Seol; Jeon, Mijeong; Kim, Seong‐Oh; Cho, Sung Won; Ruparel, Nikita B.; Song, Je Seon

doi:10.1016/j.joen.2016.01.024

Cited by 12 publications

(7 citation statements)

References 49 publications

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“…It is also possible that a more resistant biofilm is associated with the presence of a sinus tract in which the infection is well established (33). Determining the type of the stem cells contained in the apical papilla may reveal another possible predictor factor for further analysis (34,35). Determination of the stage of root formation and etiology, as discussed previously, are possible critical factors for any therapeutic decision.…”

Section: Discussionmentioning

confidence: 99%

Clinical Evidence for Regenerative Endodontic Procedures: Immediate versus Delayed Induction?

Botero

Tang

Gardner

et al. 2017

Journal of Endodontics

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Clinicians face many challenges when treating immature permanent teeth in young patients. Immediate blood clot induction can be a successful option as described by some case reports. No experimental studies or clinical trials have addressed this question. We have designed a clinical trial in which we hypothesized that there is no difference in success between immediate or delayed induction protocols. After confirmation of pulpal necrosis, patients were randomized. In the delayed group, 15 teeth were treated following the American Association of Endodontists guidelines, and calcium hydroxide was used as the intracanal medication. In the immediate group, 13 teeth had a blood clot inducted at the first appointment. The teeth were evaluated after 1, 3, and 12 months. Three independent evaluators assessed the periapical healing. The Pearson chi-square test or the Fisher exact test was used to compare the success rates between the 2 groups. Currently, of the 25 recruited patients (28 teeth), 19 have completed their 12-month follow-up. The group with delayed induction had a 71% success rate, and the group with immediate induction had a 33% success rate. In most cases (79%), trauma was the etiology. All successful cases started at stage 9 of root development (Nolla), and the majority showed healing type 2. Determination of the stage of root formation and etiology are possible critical factors for any therapeutic decision. In summary, it is early to conclude or suggest any of the protocols. Clearly, much more data are needed before sample size requirements can be met.

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Section: Discussionmentioning

confidence: 99%

Clinical Evidence for Regenerative Endodontic Procedures: Immediate versus Delayed Induction?

Botero

Tang

Gardner

et al. 2017

Journal of Endodontics

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“…The GABAA receptor has at least 19 different subunits and shows different susceptibility to drugs depending on the combination of units [29,30]. The GABAA receptor beta 1 subunit (GABRB1) is strongly expressed in the human coronal pulp and permanent predentin/odontoblasts [31,32]. Although we demonstrated that MDZ-only treatment enhanced ALP activity and mineralization induction, combinations of MDZ with BMP2 or TGF-β1 tended to decrease both of them.…”

Section: Discussionmentioning

confidence: 97%

Potential for Drug Repositioning of Midazolam for Dentin Regeneration

Karakida

Onuma

Saito

et al. 2019

IJMS

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Drug repositioning promises the advantages of reducing costs and expediting approvalschedules. An induction of the anesthetic and sedative drug; midazolam (MDZ), regulatesinhibitory neurotransmitters in the vertebrate nervous system. In this study we show the potentialfor drug repositioning of MDZ for dentin regeneration. A porcine dental pulp-derived cell line(PPU-7) that we established was cultured in MDZ-only, the combination of MDZ with bonemorphogenetic protein 2, and the combination of MDZ with transforming growth factor-beta 1. Thedifferentiation of PPU-7 into odontoblasts was investigated at the cell biological and genetic level.Mineralized nodules formed in PPU-7 were characterized at the protein and crystal engineeringlevels. The MDZ-only treatment enhanced the alkaline phosphatase activity and mRNA levels ofodontoblast differentiation marker genes, and precipitated nodule formation containing a dentinspecificprotein (dentin phosphoprotein). The nodules consisted of randomly orientedhydroxyapatite nanorods and nanoparticles. The morphology, orientation, and chemicalcomposition of the hydroxyapatite crystals were similar to those of hydroxyapatite that hadtransformed from amorphous calcium phosphate nanoparticles, as well as the hydroxyapatite inhuman molar dentin. Our investigation showed that a combination of MDZ and PPU-7 cellspossesses high potential of drug repositioning for dentin regeneration.

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“…In contrast, apical papilla cells are never able to form a calcified matrix even when they are in contact with a specific dentinoinductive influence (41). A study that compared the complex gene expression in the coronal pulp and apical pulp complex in human immature teeth found that genes related to dentin mineralization were expressed more strongly in the coronal pulp than the apical pulp (16). Taken together with the results of this study, this suggests that cytokines expressed in DPSCs provide a more suitable environment for odontoblast differentiation for the same epigenetic signals.…”

Section: Discussionmentioning

confidence: 99%

“…Cytokines commonly expressed in DSCs are involved in various mechanisms, such as dental development, neurogenesis, and inflammatory responses, and regeneration of the dentin-pulp complex (12)(13)(14)(15). Previous studies comparing the cell proliferation, mineralization capacity, and gene expression of DPSCs and DACCs showed that these 2 regions of cells play different roles (6,16). It can therefore be inferred that the cytokines secreted by each cell type are also different.…”

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confidence: 99%

Cytokine Expression of Stem Cells Originating from the Apical Complex and Coronal Pulp of Immature Teeth

Joo

Song

Kim

et al. 2018

Journal of Endodontics

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Comparative Gene Expression Analysis of the Coronal Pulp and Apical Pulp Complex in Human Immature Teeth

Cited by 12 publications

References 49 publications

Clinical Evidence for Regenerative Endodontic Procedures: Immediate versus Delayed Induction?

Clinical Evidence for Regenerative Endodontic Procedures: Immediate versus Delayed Induction?

Potential for Drug Repositioning of Midazolam for Dentin Regeneration

Cytokine Expression of Stem Cells Originating from the Apical Complex and Coronal Pulp of Immature Teeth

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