Comparative genetic diversity of wild and hachery populations of Korean threadsail filefish Stephanolepis cirrhifer using cross-species microsatellite markers

Abstract

The threadsail filefish Stephanolepis cirrhifer is a highly commercial fisheries resource in Korea that suffers intensive anthropogenic pressure across much of its range. For basic information about its current genetic status in relation to stock enhancement, the level and distribution of genetic variation between a wild and a hatchery-bred population were investigated using 10 microsatellite markers developed for Thamnaconus modestus. High levels of polymorphism were observed between the two populations. A total of 95 different alleles were found at all loci, with some alleles being unique. The allelic variability ranged from six to 13 in the wild population and from five to 13 in the hatchery one. The average observed and expected heterozygosities were estimated to be 0.72 and 0.80 in the wild sample and 0.70 and 0.79 in the hatchery one, respectively. These results showed similar genetic variability in the hatchery population, as compared with the wild population and significant genetic differentiation between the wild population and the hatchery samples (FST = 0.016, P < 0.05). Genetic drift in the intensive breeding practices for stock enhancement has probably promoted differentiation between populations. Significant deviations from Hardy-Weinberg equilibrium were detected in both populations. Our results indicate that further studies using species-specific microsatellite markers will be necessary for a more reliable assessment of genetic diversity of the species.

Mitochondrial and microsatellite DNA analyses showed comparative genetic diversity between parent and offspring populations of Korean black rockfish in a hatchery facility

“…A similar phenomenon was also observed in a previous microsatellite analysis (An et al, 2011a). It is possible that null or non-amplifying alleles could account for the heterozygote deficiencies in the data.…”

“…Although allelic diversity in terms of the number of alleles per locus has been shown to be variable depending on the sample size, the sample size of offspring in this study was larger than that of the parent population. We found that genetic diversity was similar in S. inermis, but the number of alleles per locus was relatively higher than that reported by An et al (2011a). Because allele number is positively correlated with sample size (Liu et al, 2009), the number of alleles observed in this study was probably due to the relatively large sample size.…”

Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers

“…Conservation of genetic diversity is important for the long-term objective for marine species (Falk & Holsinger, 1991). Although many studies have found significant reductions in genetic diversity of offspring compared with broodstock, no significant difference was detected between broodstock I and offspring in this study (Reily et al , 1999; An et al , 2011a, b). High levels of genetic diversity in wild populations of fish can be related to large effective population size, environmental heterogeneity, life history traits and genetic divergence (Nei, 1987; Xiao et al , 2016).…”

Genetic differences between broodstock and offspring of seven-band grouper in a hatchery

“…However, nonrandom mating, and/or artificial selection forces in play during seed production, may account for any deviation from HWE in broodstock. In offspring, heterozygote deficiency is commonly caused by a limited number of founders, inbreeding, or both (Kohlmann et al 2005;An et al 2011b). Such a deficit may also be attributable to inappropriate domestication of hatchery populations.…”