Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea

Trantas, Emmanouil; Licciardello, Grazia; Almeida, Nalvo F.; Witek, Kamil; Strano, C. P.; Duxbury, Zane; Ververidis, Filippos; Goumas, Dimitrios E.; Jones, Jonathan D. G.; Guttman, David S.; Catara, Vittoria; Sarris, Panagiotis F.

doi:10.3389/fmicb.2015.00811

Cited by 39 publications

(43 citation statements)

References 90 publications

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“…Ralstonia solanacearum is a pathogenic agent of bacterial wilt23; Pseudomonas syringae, P. aeruginosa, P. corrugate and P. mediterranea are economically important plant pathogens242526; Rhizoctonia solani is a soil-borne fungal pathogen that causes disease in a wide range of plants including tobacco27; Fusarium contains many plant pathogens such as F. oxysporumi for infection of tomato and F. solani for infection of pea28. It is possible that these pathogenic microorganisms may cause serious plant diseases in the degraded soils, consistent with the high disease index of tobacco bacteria wilt in the degraded soils (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Microbial taxa and functional genes shift in degraded soil with bacterial wilt

Zhang

Wang

Shu

et al. 2017

Sci Rep

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Soil degradation is a serious global problem, but little is known about how soil microbial communities respond to soil degradation as well as their feedback to ecosystem functioning. In this study, we found the microbial community composition, structure and functional potential significantly altered in the degraded soils with bacterial wilt (termed as degraded soils). Compared with healthy soils, OTU richness of beneficial microorganisms were significantly decreased, but OTU richness of pathogenic microorganisms were significantly increased in the degraded soils. Functional gene array (GeoChip 5.0) analysis showed the functional metabolic potential of genes involved in stress, virulence, sulfur cycle, metal resistance, degradation of plant cell wall was significantly increased in the degraded soils. Increased functional metabolic potential of these genes may be related to the acidification and severe plant disease of degraded soils. Biological activity of degraded soils was obviously decreased with weakened soil enzyme activities when compared to the healthy soils. Soil pH and enzyme activities were negatively correlated with the abundance of genes involved in sulfur cycle, virulence, and stress responses. This study provides new insights into our understanding of soil microbial community responses to soil degradation.

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Section: Discussionmentioning

confidence: 99%

Microbial taxa and functional genes shift in degraded soil with bacterial wilt

Zhang

Wang

Shu

et al. 2017

Sci Rep

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“…As seen in other non-pathogenic and pathogenic pseudomonads, a mangotoxin-like biosynthetic cluster is conserved in P. brassicacearum, P. corrugata and P. mediterranea (Fig. 1) [20,41,52,53]. Despite its name, the product of this mangotoxin-like cluster is thought to act as a regulator of SMs rather than a toxin [41,54,53].…”

Section: Mangotoxinmentioning

confidence: 97%

Friend or foe? Exploring the fine line between Pseudomonas brassicacearum and phytopathogens

Gislason

Kievit

2020

Journal of Medical Microbiology

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Pseudomonas brassicacearum is one of over fifty species of bacteria classified into the P. fluorescens group. Generally considered a harmless commensal, these bacteria are studied for their plant-growth promotion (PGP) and biocontrol characteristics. Intriguingly, P. brassicacearum is closely related to P. corrugata , which is classified as an opportunistic phytopathogen. Twenty-one P. brassicacearum genomes have been sequenced to date. In the current review, genomes of P. brassicacearum and strains from the P. corrugata clade were mined for regions associated with PGP, biocontrol and pathogenicity. We discovered that ‘beneficial’ bacteria and those classified as plant pathogens have many genes in common; thus, only a fine line separates beneficial/harmless commensals from those capable of causing disease in plants. The genotype and physiological state of the plant, the presence of biotic/abiotic stressors, and the ability of bacteria to manipulate the plant immune system collectively contribute to how the bacterial-plant interaction plays out. Because production of extracellular metabolites is energetically costly, these compounds are expected to impart a fitness advantage to the producer. P. brassicacearum is able to reduce the threat of nematode predation through release of metabolites involved in biocontrol. Moreover this bacterium has the unique ability to form biofilms on the head of Caenorhabditis elegans, as a second mechanism of predator avoidance. Rhizobacteria, plants, fungi, and microfaunal predators have occupied a shared niche for millions of years and, in many ways, they function as a single organism. Accordingly, it is essential that we appreciate the dynamic interplay among these members of the community.

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“…P. mediterranea, a pathogen responsible for pith necrosis, was detected predominantly in winter, with decreasing in the nutrient solution but increasing in the substrate. Pith necrosis is a disease of tomato that causes severe crop losses [31,32]. Additionally, some potentially pathogenic fungi including F. oxysporum and A. alternate, were increased considerably in the nutrient solution or substrate samples.…”

Section: Discussionmentioning

confidence: 99%

Microbial community dynamics in the recirculating nutrient solution of tomato plug seedlings cultivated under ebb-and-flow system

Chen¹,

Li²,

Wang³

et al. 2019

Preprint

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Background: The ebb-and-flow system has ability to recirculate water and nutrients, and offers a good method to control nutrient leaching from greenhouses into the environment. However, the potential for the rapid spread of bacterial and fungal pathogens is the main hindrance for its adoption in vegetable seedlings production. Natural microflora has often shown a certain ability to suppress diseases.Results: Here, through 16S rRNA- and ITS1-targeted Illumina sequencing, the dynamic changes in bacterial and fungal communities in the recirculating nutrient solution were characterized for tomato plug seedlings cultivated in an ebb-and-flow system in summer and winter. Both bacterial number and microbial diversity in the nutrient solution increased with recirculating irrigation, and these changes differed between summer and winter. Pseudomonas was among the most predominant bacterial genera in the nutrient solution; its relative abundance gradually increased with recycling in summer but decreased dramatically in winter. In summer, the predominant bacteria also included Comamonas and Sediminibacterium , whose relative abundances in the nutrient solution decreased and increased, respectively. In winter, the nutrient solution was also colonized by Sphingomonas , predominantly at the late stage of irrigation. Among fungi, Amanita and Trichoderma were predominant in both summer and winter. Amanita gradually increased in summer but decreased rapidly after the first irrigation cycle in winter, whereas Trichoderma accumulated rapidly at the late stage of irrigation in both summer and winter. Alternaria and Fusarium were predominant in the nutrient solution only in summer, with the former decreased and the latter increased with recirculating irrigation. Moreover, Pseudomonas mediterranea , a potentially pathogenic bacterium, and Fusarium oxysporum , a fungal pathogen were present predominantly in winter and summer, respectively. Some potentially beneficial microbes functioning in plant-growth promotion and water self-purification, such as Sphingobium xenophagum , Trichoderma harzianum , and T. virens , were identified to be increased in the recirculating nutrient solution.Conclusions: The present data elucidate the bacterial and fungal dynamics in an ebb-and-flow system and provide useful information for pathogen control during tomato seedling production.

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Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea

Cited by 39 publications

References 90 publications

Microbial taxa and functional genes shift in degraded soil with bacterial wilt

Microbial taxa and functional genes shift in degraded soil with bacterial wilt

Friend or foe? Exploring the fine line between Pseudomonas brassicacearum and phytopathogens

Microbial community dynamics in the recirculating nutrient solution of tomato plug seedlings cultivated under ebb-and-flow system

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