Comparative genomic analysis of the first Ehrlichia canis detections in Australia

Neave, Matthew J.; Mileto, Patrick; Joseph, Ancy; REID, Tristan; Scott, Angela C; Keyburn, Anthony L.

doi:10.1016/j.ttbdis.2022.101909

Cited by 19 publications

(16 citation statements)

References 36 publications

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“…Moreover, the use of hybrid assembly strategies, combining long-and short-read sequencing, is recommended for more complete and accurate reference genome assemblies [19,[22][23][24][25], especially when repetitive elements are expected. This approach has already been used for other members of the Anaplasmataceae in order to assemble high-resolution whole genomes [26,27]. The long reads provide reliable contigs allowing for repeat regions and complex sequences to be structurally correct, and polishing with accurate short reads corrects errors in the assembly [22,23,28].…”

Section: Introductionmentioning

confidence: 99%

Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes

Azagi

Dirks²,

Yebra-Pimentel³

et al. 2022

Microorganisms

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Ca. Neoehrlichia mikurensis is widely prevalent in I. ricinus across Europe and has been associated with human disease. However, diagnostic modalities are limited, and much is still unknown about its biology. Here, we present the first complete Ca. Neoehrlichia mikurensis genomes directly derived from wildlife reservoir host tissues, using both long- and short-read sequencing technologies. This pragmatic approach provides an alternative to obtaining sufficient material from clinical cases, a difficult task for emerging infectious diseases, and to expensive and challenging bacterial isolation and culture methods. Both genomes exhibit a larger chromosome than the currently available Ca. Neoehrlichia mikurensis genomes and expand the ability to find new targets for the development of supportive laboratory diagnostics in the future. Moreover, this method could be utilized for other tick-borne pathogens that are difficult to culture.

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Section: Introductionmentioning

confidence: 99%

Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes

Azagi

Dirks²,

Yebra-Pimentel³

et al. 2022

Microorganisms

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“…Mild LGL lymphocytosis can occur with any inflammatory process and marked LGL lymphocytosis has been reported with chronic Ehrlichia canis infection 36 . E. canis was first reported in Australia in May 2020 37 . At the time of this study, E. canis was not endemic in the geographical area from which these cases were sourced, limiting the possibility of persistent LGL lymphocytosis from non‐neoplastic cause in the study population.…”

Section: Discussionmentioning

confidence: 84%

“…36 E. canis was first reported in Australia in May 2020. 37 At the time of this study, E. canis was not endemic in the geographical area from which these cases were sourced, limiting the possibility of persistent LGL lymphocytosis from non-neoplastic cause in the study population. It cannot be excluded however that cases with reactive causes of increased peripheral LGLs may be included based on the criteria adopted for this study.…”

Section: Discussionmentioning

confidence: 91%

Clinicopathologic and immunophenotypic features in dogs with presumptive large granular lymphocyte leukaemia

Jaensch¹,

Hayward²,

Boyd

2022

Aust Veterinary J

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Large granular lymphocytic leukaemia (LGLL) has been described in a range of species but has been most commonly reported in humans and dogs. In both species, this neoplasia exhibits diversity in both phenotype and biological behaviour with phenotype only partially predicting behaviour. There is currently little knowledge of concurrent haematological and serum biochemistry features or concurrent occurrence of distinct neoplasia in canine LGLL cases. This study presents a canine case series and defines haematological parameters, novel serum biochemistry findings and phenotype of the large granular lymphocytes in an Australian case series. Neutrophilia was the most common haematological abnormality, identified in 43% of dogs, and 84% of dogs with biochemistry data available had elevated serum gamma-glutamyl transferase. Five of the 40 dogs in this study exhibited concurrent neoplasia during the period of the study, demonstrating this is a relatively common clinical outcome in canine LGLL cases. In agreement with previous canine and human studies, the most common LGLL phenotype in dogs is CD3+, CD4À and CD8+. Further work is needed to define the variables predictive of the biological behaviour of LGLL in dogs.

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“…It has also been reported in some other tick species such as R. microplus , R. linnaei , R. bursa , etc. [ 10 , 22 , 23 ]. As the causative agent of canine monocytic ehrlichiosis (CME), E. canis is one of the most prevalent tick-borne pathogens infecting dogs worldwide.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Rickettsiales in Rhipicephalus microplus Ticks Collected in Domestic Ruminants in Guizhou Province, China

Meng

Gao³

et al. 2022

Pathogens

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Rhipicephalus microplus ticks are vectors for multiple pathogens infecting animals and humans. Although the medical importance of R. microplus has been well-recognized and studied in most areas of China, the occurrence of tick-borne Rickettsiales has seldom been investigated in Guizhou Province, Southwest China. In this study, we collected 276 R. microplus ticks from cattle (209 ticks) and goats (67 ticks) in three locations of Guizhou Province. The Rickettsia, Anaplasma, and Ehrlichia were detected by targeting the 16S rRNA gene and were further characterized by amplifying the key genes. One Rickettsia (Ca. Rickettsia jingxinensis), three Ehrlichia (E. canis, E. minasensis, Ehrlichia sp.), and four Anaplasma (A. capra, A. ovis, A. marginale, Ca. Anaplasma boleense) species were detected, and their gltA and groEL genes were recovered. Candidatus Rickettsia jingxinensis, a spotted fever group of Rickettsia, was detected in a high proportion of the tested ticks (88.89%, 100%, and 100% in ticks from the three locations, respectively), suggesting the possibility that animals may be exposed to this type of Rickettsia. All the 16S, gltA, groEL, and ompA sequences of these strains are 100% identical to strains reported in Ngawa, Sichuan Province. E. minasensis, A. marginale, and Candidatus Anaplasma boleense are known to infect livestock such as cattle. The potential effects on local husbandry should be considered. Notably, E. canis, A. ovis, and A. capra have been reported to infect humans. The relatively high positive rates in Qianxinan (20.99%, 9.88%, and 4.94%, respectively) may indicate the potential risk to local populations. Furthermore, the genetic analysis indicated that the E. minasensis strains in this study may represent a variant or recombinant. Our results indicated the extensive diversity of Rickettsiales in R. microplus ticks from Guizhou Province. The possible occurrence of rickettsiosis, ehrlichiosis, and anaplasmosis in humans and domestic animals in this area should be further considered and investigated.

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Comparative genomic analysis of the first Ehrlichia canis detections in Australia

Cited by 19 publications

References 36 publications

Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes

Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes

Clinicopathologic and immunophenotypic features in dogs with presumptive large granular lymphocyte leukaemia

Diversity of Rickettsiales in Rhipicephalus microplus Ticks Collected in Domestic Ruminants in Guizhou Province, China

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