Comparative genomic hybridization and amplotyping by arbitrarily primed PCR in stage A B-CLL

Abstract

Cytogenetic analysis is useful in the diagnosis and to assess prognosis of B-cell chronic lymphocytic leukemia (B-CLL). However, successful cytogenetics by standard techniques has been hindered by the low in vitro mitotic activity of the malignant B-cell population. Fluorescence in situ hybridization (FISH) has become a useful tool, but it does not provide an overall view of the aberrations. To overcome this hurdle, two DNA-based techniques have been tested in the present study: comparative genomic hybridization (CGH) and amplotyping by arbitrarily primed PCR (AP-PCR). Comparative genomic hybridization resolution depends upon the 400-bands of the human standard karyotype. AP-PCR allows detection of allelic losses and gains in tumor cells by PCR fingerprinting, thus its resolution is at the molecular level. Both techniques were performed in 23 patients with stage A B-CLL at diagnosis. The results were compared with FISH. The sensitivity of AP-PCR was greater than CGH (62% vs 43%). The use of CGH combined with AP-PCR allowed to detect genetic abnormalities in 79% (15/19) of patients in whom G-banding was not informative, providing a global view of the aberrations in a sole experiment. This study shows that combining these two methods with FISH, makes possible a more precise genetic characterization of patients with B-CLL.

Novel genomic alterations and clonal evolution in chronic lymphocytic leukemia revealed by representational oligonucleotide microarray analysis (ROMA)

“…11,14 CGH can detect novel lesions, ascertain the frequencies of gains and losses with greater accuracy, and pinpoint candidate genes associated with the disease within known regions of recurrent abnormality. [33][34][35][37][38][39] Therefore, on the basis of previous experiences, 14,40,41 it was reasonable to expect that increased resolution would yield more accurate delineation of previously described lesions as well as identification of new ones.…”

Comparative genomic hybridization analysis of Japanese B-cell chronic lymphocytic leukemia: correlation with clinical course

“…However, in Japanese patients, we observed a lower frequency of any abnormality, particularly regarding gains of 12q, with the exception of a higher frequency of gains of 3q, compared to patients in Western countries [9]. In the report by Odero et al [18], the frequency of no imbalances was similar to that found in our study, and less than those of Bentz et al [9]. This might be associated with the exclusion of cases in Binet stage C in the former series in contrast to the latter two.The frequency of cytogenetic abnormality was high in advanced B-CLL [21,22].…”

Identification of Genomic Markers by RAPD-PCR Primer in Leukemia Patients

“…Hospital from different regions of Syria during the period (Odero et al, 2001). from 20/3/2007 to 30/12/2007 according to reported Recently, it has been reported that molecular genetic methods (Goossens et al, 1991 and dNTPs (0.4 mM of each of dATP, dCTP, dGTP, leukemia.…”