Comparative genomic provides insight into the virulence and genetic diversity of Vibrio parahaemolyticus associated with shrimp acute hepatopancreatic necrosis disease

Diagnostic approaches and contribution of next-generation sequencing technologies in genomic investigation of Vibrio parahaemolyticus that caused acute hepatopancreatic necrosis disease (AHPND)

“…In the study of the genetic diversity of VP AHPND strains in Asia and Mexico using the SNP-based method, the VP AHPND strains are genetically distinct in different localities, and there are no epidemiological links between the Asian and Mexico outbreaks (Fu et al 2017). The findings were further supported by another study that involved 28 global VP AHPND strains from different countries (Yu et al 2020). An understanding of the genetic diversity of VP AHPND strains could hasten the intervention strategies, providing information such as the global spread of pathogens and rational development of diagnostics and therapeutics and vaccines to mitigate the risk of AHPND since such data are currently lacking.…”

“…Although the pir vp toxin genes are the primary causes of AHPND, there might be other virulence components or antibiotic resistant genes that play important roles during shrimp infection. Previous studies reported that the VP AHPND strains possess a T6SS protein secretion apparatus that is known to play a role in inter-bacterial competition (Yang et al 2018 ; Yu et al 2020 ). The T6SS possesses antibacterial activities mediated by delivery of toxic effectors into neighbouring cells and therefore the acquisition T6SS might confer a fitness advantage to VP AHPND strains over other competing bacteria and facilitates shrimp infection (Yang et al 2018 ).…”

“…By using the single-nucleotide polymorphism (SNP) based on WGS data, the genetic diversity of bacteria could be accurately presented especially if established typing schemes are not available. Moreover, SNP has higher resolution than other existing sequence-based typing schemes (Schürch et al 2018 ; Yu et al 2020 ). In the study of the genetic diversity of VP AHPND strains in Asia and Mexico using the SNP-based method, the VP AHPND strains are genetically distinct in different localities, and there are no epidemiological links between the Asian and Mexico outbreaks (Fu et al 2017 ).…”

“…Also, several gene clusters such as TA system, cholera toxin and Vibrio core oligosaccharides biosynthesis were present in the Thai and Malaysian strains but absent in the genome of Vietnamese VP AHPND strain SDA4 which has the lowest virulence among the studied strains. This suggests that the identified genes might contribute to the variation in virulence among the VP AHPND strain (Yu et al 2020 ). Genetic variation is one of the problems that is often faced in the development of vaccine, and NGS could provide new critical insights into vaccine development strategies.…”

De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus

“…Takashi et al reported a negative correlation between the number of prophages and CRISPRs in Streptococcus pyogenes, indicating that CRISPR integration could limit the insertion of prophage (Nozawa et al, 2011). Comparative genomic research showed that pathogenic V. parahaemolyticus could promote the insertion of prophage when lacking CRISPR (Yu et al, 2020).…”