Comparative glucose and fructose incorporation and conversion by <i>in vitro</i> Produced bovine embryos

Guyader-Joly, C.; Khatchadourian, Chaqué; Ménézo, Yves

doi:10.1017/s0967199400002951

Cited by 22 publications

(18 citation statements)

References 19 publications

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“…Incorporation and oxidation of radiolabel glucose, fructose, and methionine by porcine oocytes All radioactive substrates, 14 C-glucose (spec. act: 185 MBq/ mmol), 14 C-fructose (spec. act: 212 MBq/mmol), and 14 C-methionine (spec.…”

Section: Assessment Of Oocyte Nuclear Status and Fertilizationmentioning

confidence: 99%

“…Like glucose, fructose is capable of entering the glycolytic pathway. In addition, fructose is present in rabbits [10] and bovine [11] reproductive tracts and effectively supports embryonic development in hamster [12], bovine [13,14], mouse [15], porcine [16], and human embryos [17]. Furthermore, fructose can increase the total cell numbers in both hamster [12] and bovine blastocysts [13].…”

Section: Introductionmentioning

confidence: 99%

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The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes

Tsujii

Lee

Hossain

et al. 2008

Reprod Medicine & Biology

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Purpose This study was conducted to investigate the effects of supplementing fructose in the culture medium on in vitro maturation (IVM), in vitro fertilization (IVF), and metabolism of porcine oocytes. Methods Porcine oocytes were matured in vitro in modified North Carolina State University 37 medium (NCSU-37) and then supplemented with either glucose (5.5 mM), fructose (5.5 mM), or glucose (2.75 mM) plus fructose (2.75 mM). The maturation and fertilization of oocytes, and the incorporation and oxidation of 14 C-glucose, 14 Cfructose, and 14 C-methionine in oocytes at different stages of development were examined. Results The supplementation of glucose plus fructose significantly promoted (P \ 0.05) oocytes germinal vesicle break down (GVBD), maturation to metaphase II (MII), penetration by spermatozoa, and male pronuclear formation compared with glucose. The incorporation and oxidation of 14 C-methionine into the oocyte significantly increased (P \ 0.05) with glucose plus fructose supplementation than glucose. A significantly higher (P \ 0.05) rate of incorporation and oxidation was achieved with 14 Cfructose compared to 14 C-glucose. Conclusions Glucose plus fructose supplementation improved maturation, penetration by spermatozoa, male pronuclear formation, and energy metabolism by porcine oocytes.

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Section: Assessment Of Oocyte Nuclear Status and Fertilizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes

Tsujii

Lee

Hossain

et al. 2008

Reprod Medicine & Biology

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“…Moreover, it has been demonstrated that inclusion of glucose in culture media affects negatively the development of early-stage embryos in hamsters (Schini & Bavister, 1988;Barnett & Bavister, 1996;Barnett et al, 1997), mice (Chatot et al, 1989;Lawitts and Biggers, 1991;Scott & Whittingham, 1996), rats (Kishi et al, 1991;Miyoshi et al, 1994), cattle (Kim et al, 1993), sheep (Thompson et al, 1992) and humans (Conaghan et al, 1993;Quinn, 1995). It has been reported that fructose is present in the reproductive tract of cattle (Suga & Masaki, 1973) and is utilized by bovine embryos in vitro (Guyader-Joly et al, 1996). As with glucose, fructose can be metabolized through the glycolytic pathway.…”

Section: Introductionmentioning

confidence: 99%

Supplementation of fructose in chemically defined protein-free medium enhances the in vitro development of bovine transgenic cloned embryos

Bhuiyan

Kang

2007

Zygote

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The present study evaluated the possible embryotrophic role of fructose supplementation in chemically defined protein-free KSOM on in vitro development of bovine transgenic cloned embryos. Bovine fetal fibroblasts transfected with expression plasmids for bovine prion protein (PrP) mutant gene with GFP marker gene were used as donor nuclei for reconstruction of slaughterhouse-derived in vitro matured oocytes. The reconstructed oocytes were cultured in KSOM supplemented with 0.01% PVA (KSOM-PVA) at 39 degrees C in a humidified atmosphere of 5% CO2, 5% O2 and 90% N2 for 192 h. In Experiment 1, when reconstructed oocytes were cultured in KSOM-PVA supplemented with glucose (0.2 mM), fructose (1.5 mM) or combined glucose and fructose (0.2 and 1.5 mM, respectively), significantly (p < 0.05) higher blastocyst (19.2%) and hatching/hatched blastocyst (13.1%) formation rates were obtained in combined fructose and glucose supplemented medium than glucose supplemented counterpart (10.0% and 5.7%, respectively). In Experiment 2, when reconstructed oocytes were cultured in KSOM-PVA supplemented with 0.0, 0.2, 1.5, 3.0 and 5.6 mM fructose in combination with 0.2 mM glucose, the blastocyst formation rate was significantly higher (17.6%) in 1.5 mM fructose supplemented group than that of no fructose supplemented counterpart (9.7%; p > 0.05). In conclusion, supplementation of combined fructose (1.5 mM) and glucose (0.2 mM) in chemically defined protein-free KSOM enhances the in vitro development of bovine transgenic cloned embryos.

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“…Some of those mechanisms could be critical for the compaction of the morula and the protection against oxidative stress (Surani, 1979;Flood & Wiebold, 1988;Wales & Hunter, 1990;Thompson et al, 1992a;Rieger, 1992;Rieger et al, 1992;Barnett et al, 1993;Guyader-Joly et al, 1996;Gardner, 1998;Khurana & Niemann, 2000). However, ruminant embryos do not seem to store glycogen (Thompson et al, 1995;Guyader-Joly, 1996). The increase in glucose uptake observed with blastocyst formation, expansion and hatching seems to be related mainly to the increasing number of cells, as no significant difference was detected if the uptake was expressed on a per cell basis.…”

Section: Discussionmentioning

confidence: 99%

“…In the mouse, the shift towards glucose use seems to depend on the developmental stage (not the age or the cell number of the embryo) and requires transcription of the embryonic genome (Martin & Leese, 1999). In various species, the morula and blastocyst stages are characterised by a further increased use of exogenous glucose for energy production but also as a precursor of triglycerides, amino acid and glycoproteins implicated in compaction (Surani, 1979;Flood & Wiebold, 1988;Wales & Hunter, 1990; Thompson et al, 1992a;Rieger, 1992;Rieger et al, 1992;Barnett et al, 1993;Guyader-Joly et al, 1996;Gardner, 1998;Khurana & Niemann, 2000). Total glucose metabolism doubles between the morula and expanded blastocyst stages in cattle embryos (Tiffin et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Impact of adding 5.5 mM glucose to SOF medium on the development, metabolism and quality of in vitro produced bovine embryos from the morula to the blastocyst stage

Donnay

Feugang

Bernard

et al. 2002

Zygote

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Although toxic for early stages of embryo development, glucose is a physiological metabolic substrate at the morula and blastocyst stages. We evaluated the effect of adding 5.5 mM glucose from the morula stage on bovine blastocyst development and quality. In vitro matured and fertilised bovine oocytes were cultured in modified Synthetic Oviduct Fluid medium containing 5% fetal calf serum, but without added glucose, up to day 5 post-insemination (pi). Morulae were selected and further cultured in the presence or absence of 5.5 mM glucose. Blastocyst and hatched blastocyst rates were recorded. Oxygen, glucose and pyruvate uptakes as well as lactate release were evaluated. The quality of the resulting blastocysts was evaluated by the cell allocation to the inner cell mass (ICM) and trophectoderm (TE) and by the apoptotic index. Adding glucose increased the blastocyst rate at day 8 pi (80% vs 65%) but had no impact on hatching rate (25% vs 28%). A 22% decrease in oxygen uptake was observed in the presence of glucose, concomitant with an increase in lactate release, although no change was observed in pyruvate uptake. A slight decrease in blastocyst cell number was observed at day 7 in the presence of glucose while neither the ICM/TE cell ratio nor the apoptotic index were affected. In conclusion, adding 5.5 mM glucose from the morula stage has a limited impact on blastocyst rate and quality although important modifications were observed in embryo metabolism. It remains to be determined whether those modifications could influence embryo viability after transfer.

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Comparative glucose and fructose incorporation and conversion by in vitro Produced bovine embryos

Cited by 22 publications

References 19 publications

The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes

The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes

Supplementation of fructose in chemically defined protein-free medium enhances the in vitro development of bovine transgenic cloned embryos

Impact of adding 5.5 mM glucose to SOF medium on the development, metabolism and quality of in vitro produced bovine embryos from the morula to the blastocyst stage

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