Comparative haematological parameters of HbAA and HbAS genotype children infected with<i>Plasmodium falciparum</i>malaria in Yemen

Albiti, Anisa H.; Nsiah, Kwabena

doi:10.1179/1607845413y.0000000113

Cited by 16 publications

(28 citation statements)

References 33 publications

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“…A comparison of these frequencies indicated that the percentage of SCA picked up by neonatal screening in children with severe anaemia ranged from 1% globally to 3% in Kinshasa. This may be due to the fact that haemoglobin-AS protected children from malaria and this, in turn, reduced the frequency of children with severe anaemia that had the sickle cell trait, as reported in previous studies (24,25).…”

Section: Discussionsupporting

confidence: 63%

High rate of sickle cell anaemia in Sub‐Saharan Africa underlines the need to screen all children with severe anaemia for the disease

et al. 2015

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Section: Discussionsupporting

confidence: 63%

High rate of sickle cell anaemia in Sub‐Saharan Africa underlines the need to screen all children with severe anaemia for the disease

et al. 2015

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“…The results of this study revealed that 73% of the malaria positive participants had the haemoglobin genotype AA (HbAA), which was higher than the percentages in the other genotypes (HbAS [23%]) and HbAC [3%]). This is similar to the reports of Albiti and Nsiah [40] and Akanbi [39]. The prevalence of participants with HbAS and HbAC were quite low compared to HbAA.…”

Section: Discussionsupporting

confidence: 90%

Genetic markers associated with antimalarial drug resistance and haemoglobin genotypes among malaria patients in Kaduna State, Nigeria

Benjamin

Inabo

Muhammad

et al. 2020

Preprint

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Background Malaria is a disease of public health concern in Nigeria and sub-Saharan Africa. The emergence of drug resistance, particularly among P. falciparum strains, has been a major contributor to the global burden of malaria. This research was aimed at detecting genetic markers (pfcrt, pfmdr1, pfdhfr, pfdhps, pfatpase6) associated with antimalarial drug resistance and assessing the distribution of haemoglobin genotypes among malaria patients in of Kaduna State, Nigeria. Methods Three hundred (300) blood samples were collected from consenting individuals attending selected hospitals, in the three senatorial districts of Kaduna State, Nigeria. A structured questionnaire was used to obtain relevant data from the study participants. The samples were screened for malaria parasites by microscopy and malaria rapid diagnostic test kit. Deoxyribonucleic acid was extracted from one third of the malaria positive samples, and Polymerase Chain Reaction (PCR) was used for detection of the drug resistance genes. Pfcrt, pfmdr1, pfdhfr, pfdhps and pfatpase6 genes were detected at expected amplicon sizes from the malaria positive samples. The pfatpase6 PCR amplicons were sequenced and a phylogenetic tree was created using MEGA X to determine their relatedness to published sequences. Results Pfcrt (80%) had the highest prevalence, followed by pfdhfr (60%), pfmdr1 (36%) and pfdhps (8%). Pfatpase6 was also detected in 73.3% of the samples. The phylogenetic tree showed that all the pfatpase6 gene sequences (both the ones from this study and those published in NCBI Genbank) had the same origin and were closely related. However, the sequences from NCBI Genbank were from one clade; arising from a common ancestor (monophyletic) thus they were more closely related to themselves, than to the pfatpase6 sequences obtained in this study. Of all the malaria positive participants, those with HbAA (73%) haemoglobin genotype had the highest percentage followed by HbAS (23%), HbAC (3%) and HbSS (1). Conclusion We detected Plasmodium falciparum genes associated with drug resistance to commonly used antimalarials in the study area. Expression of these genes could have serious consequences in the treatment of malaria. The percentage of Plasmodium falciparum malaria was higher among persons with HbAA than those with HbAS, HbAC and HbS.

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“…Furthermore, such alterations have also been common in malaria, and reported to be more pronounced in P. falciparum infection for the HbAA genotype (21,38). However, the magnitude of such alterations differ depending on genetic, and epigenetic factors (22). Our study provides a unique approach by not only looking at the comparisons of the haematological parameters in sickle cell genotypes in P. falciparum infection but also determines the association of these parameters to sickle cell gene in the infection.…”

Section: Discussionmentioning

confidence: 99%

“…Our study provides a unique approach by not only looking at the comparisons of the haematological parameters in sickle cell genotypes in P. falciparum infection but also determines the association of these parameters to sickle cell gene in the infection. A few studies have been carried out in African countries where malaria is holoendemic with varied demonstrations on the protective effect of sickle cell gene (HbAS and HbSS) of asymptomatic and symptomatic P. falciparum infection of variable severity (14,20,22,23). None of the above studies have speci cally described haematological differences on the basis of sickle cell genotypes in children presenting with steady state of P. falciparum malaria apart from Prohit et al (24) and…”

Section: Discussionmentioning

confidence: 99%

“…Malaria confers a selective heterozygous survival advantage to carriers of the HbS gene and this is referred to as balanced polymorphism (18). Based on previous studies, it is plausible that both P. falciparum malaria and sickle cell disease have independent signi cant impacts on the alteration of haematological parameters (21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

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Association Between Haematological Parameters and Sickle Cell Genotypes in Children With Plasmodium Falciparum Malaria Residents in Kisumu County in Western Kenya

Kosiyo

Otieno

Gitaka

et al. 2020

Preprint

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Background: Sickle cell disease (SCD) is a monogenic disorder due to point mutation in the β-globin gene resulting in substitution of Valine for Glutamic acid. The SCD is prevalent in P. falciparum endemic regions such as western Kenya. Carriage of different sickle cell genotypes may influence haematological parameter during malaria. Children resident in malaria holoendemic regions suffer more from malaria-related complications and this is moderated by the presence of the SCD. In the current study, we determined the association between sickle cell genotypes and haematological parameters in children with P. falciparum malaria resident in Kisumu County in Western Kenya.Methodology: Children (n=217, aged 1-192 months) with acute febrile condition were recruited at Jaramogi Oginga Odinga Teaching and Referral Hospital. Chi-square (2) analysis was used to determine differences between proportions. Differences in haematological parameters were compared across groups using Kruskal Wallis test and between groups using Mann Whitney U test. Multivariate logistic regression analysis controlling for infection status was used to determine the association between sickle cell genotypes and haematological parameters. Results: Using HbAA as the reference group, multivariate logistic regression analysis revealed that carriage of HbSS was associated with reduced haemoglobin [OR=0.310, 95% CI=0.101-0.956, P=0.041], reduced haematocrit [OR=0.318, 95% CI=0.128-0.793, P=0.014], reduced RBC count [OR=0.124, 95% CI=0.045-0.337, P=0.001], reduced MCHC [OR=0.325, 95% CI=0.118-0.892, P=0.029], increased leucocytosis [OR=9.283, 95% CI=3.167-27.210, P=0.001] and reduced monocytosis [OR=0.319, 95% CI=0.123-0.830, P=0.019]. However, carriage of HbAS was only associated with increased micro-platelets [OR=3.629, 95% CI=1.291-8.276, P=0.012]. Conclusion: Results show that carriage of HbSS in children influence the levels of haemoglobin, haematocrit, RBC, MCHC, WBC and Monocytes. Therefore prior knowledge of HbSS should be considered to improve clinical management of haematological alterations during malaria in children.

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Comparative haematological parameters of HbAA and HbAS genotype children infected withPlasmodium falciparummalaria in Yemen

Cited by 16 publications

References 33 publications

High rate of sickle cell anaemia in Sub‐Saharan Africa underlines the need to screen all children with severe anaemia for the disease

High rate of sickle cell anaemia in Sub‐Saharan Africa underlines the need to screen all children with severe anaemia for the disease

Genetic markers associated with antimalarial drug resistance and haemoglobin genotypes among malaria patients in Kaduna State, Nigeria

Association Between Haematological Parameters and Sickle Cell Genotypes in Children With Plasmodium Falciparum Malaria Residents in Kisumu County in Western Kenya

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