Comparative histochemistry of the gastric mucosa: A survey of the common laboratory animals and man

Abstract

A comparative histological and histochemical study of the gastric mucosa was done with the frog, mouse, rat, hamster, guinea pig, rabbit, cat, dog and man. Enzymatic histochemical studies were done to demonstrate acid phosphatase (PbS and azo-dye methods), thiamine pyrophosphatase (TPP-ase) and NADHz tetrazolium reductase activity. Cytoplasmic organelles were stained in some cases by three of the above methods i.e. acid phosphatase (PbS) activity marked the lysosomes, TPP-ase activity marked the Golgi apparatus and NADHz tetrazolium reductase activity marked the mitochondria. The acid phosphatase activity of the four major cell types was markedly different in the various species. The activity shown by both methods in individuals of a given species coincided well except in the rabbit and the dog. TPP-ase activity demonstrated the Golgi region in the surface cells of all species except the guinea pig and the frog. The Golgi zones of zymogen cells showed strong activity only in the cat, while the parietal cell Golgi was not demonstrated in any of the species. NADHz tetrazolium reductase activity in the parietal cells was strong in all animals except the mouse and the rat where staining was faint. The relationship between the lysosomes and the Golgi apparatus, as demonstrated histochemically in the gastric cells of the nine species, is discussed. The functional significance of these different enzymatic patterns has yet to be determined.

Gastroscopic and Histochemical Study of Normal, Atrophic and Hypertrophic Mucosa

“…C orreia et al [2], R agins et al [16], F loch et al [7]. We observed AcP to be most promi nent in the chief cells like P lan teyot et al [15], C orreia et at.…”

“…We observed AcP to be most promi nent in the chief cells like P lan teyot et al [15], C orreia et at. [2], R agins et al [16], and F lo ch et at. [7].…”

Enzyme Histochemical Investigations of Heterotopic Gastric Epithelium in the Duodenum

“…The results of the reactions applied to the cells in their normal position are in close agreement with previous investigations ( Villareal & Burgas 1955, Wattenberg 1959, Planteydt & Willighagen 1960, Niemi et al 1961, Plosscowe et al 1963, Ragins et al 1964, Ragins & Dittbrenner 1965. The slight reaction of the parietal cells and the surface epithelium with the non-specific esterase reaction has also been noticed by Comori using the alpha-naphthyl acetate method (Pearse 1960).…”

Intracellular enzymatic histochemistry of the human stomach with special reference to atrophic gastritis.

“…Although at least 20 different enzymes have been investigated histochemically in the stomach of man and several animals during the last nine years (summarized by Ragins, Dittbrenner, and Diaz, 1964) the vast majority of the techniques employed in these investigations used completely unfixed tissues. In an effort to preserve the intracellular structure as well as the enzymatic activity, the present study used tissues which were fixed immediately in cold neutral formalin for 24 hours, as suggested by Novikoff (1960).…”