Comparative histology of pulmonary neuroendocrine cell system in mammalian lungs

Scheuermann, D.W.

doi:10.1002/(sici)1097-0029(19970401)37:1<31::aid-jemt4>3.3.co;2-5

Cited by 9 publications

(11 citation statements)

References 95 publications

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“…5‐Hydroxytryptamine (5‐HT) or serotonin has been implicated in a variety of physiological functions in the airways of humans and experimental animals. Serotonin within most mammalian airways, including humans, originates mainly from platelets and neuroendocrine cells (Lauweryns et al 1974; Scheuermann, 1997; Cazzola & Matera, 2000; Fu et al 2002). In the healthy airway of rodents, mast cells are the main source of serotonin (Weigand et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Activation of mouse bronchopulmonary C‐fibres by serotonin and allergen‐ovalbumin challenge

Potenzieri¹,

Meeker²,

Undem³

2012

The Journal of Physiology

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Key points• Mast cell-derived serotonin is a principal mediator of allergic reactions in rodents. Serotonin can also be released from platelets during various pathological conditions. • Vagal C-fibres innervating the respiratory tract can be subdivided into nodose (placodal) C-fibres or jugular (neural crest) C-fibres). Activation of vagal placodal and/or neural crest vagal C-fibres probably contributes to the dyspnoea, cough and reflex parasympathetic drive commonly associated with serotonin.• Serotonin used different receptor subtypes to evoke strong action potential discharge in placodal (5-HT3 receptors) versus neural crest (non-5-HT3 receptors)-derived vagal C-fibres in the mouse lung.• Mast cell derived serotonin may activate neural crest C-fibres, but did not stimulate placodal C-fibres.• The effect of extracellular serotonin on vagal afferent activation therefore depends on its cellular source and the C-fibre phenotype.Abstract The effect of serotonin on capsaicin-sensitive vagal C-fibre afferent nerves was evaluated in an ex vivo vagally innervated mouse lung preparation. Action potentials arising from receptive fields in the lungs were recorded with an extracellular electrode positioned in the nodose/jugular ganglion. Among the 62 capsaicin-sensitive C-fibres studied (conduction velocity ∼0.5 m s −1 ), 71% were of the nodose phenotype and 29% of the jugular phenotype. The nodose C-fibres responded strongly to serotonin and this effect was blocked with the 5-HT3-receptor antagonist ondansetron. Using single cell RT-PCR, we noted that the vast majority of nodose neurons retrogradely labelled from the lung, expressed 5-HT3 receptor mRNA. The jugular C-fibres also responded strongly to serotonin with action potential discharge, but this effect was not inhibited by ondansetron. Lung-specific jugular neurons did not express 5-HT3 receptor mRNA but frequently expressed 5-HT1 or 5-HT4 receptor mRNA. Mast cells are the major source of serotonin in healthy murine airways. Ovalbumin-induced mast cell activation in actively sensitized lungs caused action potential discharge in jugular but not nodose C-fibres. The data show that vagal C-fibres in the respiratory tract of the mouse are strongly activated by serotonin. Depending on the C-fibre subtype both 5-HT3 and non-5-HT3 mechanisms are involved.

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Section: Introductionmentioning

confidence: 99%

Activation of mouse bronchopulmonary C‐fibres by serotonin and allergen‐ovalbumin challenge

Potenzieri¹,

Meeker²,

Undem³

2012

The Journal of Physiology

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“…The close localization of serotonin‐positive NEE cells with ciliary and mucous cells is highly indicative of some function of NEE cells. Serotonin induces mucus secretion in the digestive system of mammals (Menguy, 1967; Plaisancie et al ., 1998) and has been suggested to increase mucus secretion and ciliary beat frequency, as well as to be a potent vasoconstrictor in the respiratory system (Dey et al ., 1981; Adriaensen et al ., 1994a, 1994b; Scheuermann, 1997a, 1997b). Furthermore, immunocytochemical studies have revealed the colocalization of serotonin and neuropeptides in the same NEE cells, such as calcitonin (Adriaensen & Scheuermann, 1993), CGRP (Adriaensen & Scheuermann, 1993), SP (Adriaensen & Scheuermann, 1993), bombesin (Dayer et al ., 1985; Dey & Hoffpauir, 1986) and somatostatin (Dayer et al ., 1985).…”

Section: Discussionmentioning

confidence: 99%

Structure of the respiratory tract of the red-bellied newt Cynops pyrrhogaster, with reference to serotonin-positive neuroepithelial endocrine cells

et al. 2005

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To investigate the distribution of serotonin-positive neuroepithelial endocrine (NEE) cells in the respiratory tract of the red-bellied newt Cynops pyrrhogaster, serial sections of the respiratory tract were stained using immunohistochemical methods for serotonin, a periodic acid-Schiff method and hematoxylin-eosin staining. The respiratory tract of the red-bellied newt consisted of a tubular laryngotrachea and two pulmonary sacs. Serotonin-positive solitary NEE cells were distributed widely throughout the ciliomucous epithelium of the laryngotrachea. The innervated clusters of NEE cells, termed neuroepithelial bodies, were rarely found in the caudal portion of the laryngotrachea. The density of NEE cells was high in the middle portion of the laryngotrachea, particularly in the region surrounded by a striated laryngeal sphincter, and the densities were low cranially towards the aditus laryngis and caudally in the pulmonary sacs, the epithelium of which consisted of both ciliomucous epithelial cells and non-ciliated respiratory epithelial cells or pneumocytes. The NEE cells were not observed in the vicinity of non-ciliated respiratory epithelial cells. The morphology and distribution of serotonin-positive NEE cells in the respiratory tract of the red-bellied newt were similar to those described in mammals, including humans. The close localization of NEE cells with ciliary and mucous cells and the distribution of NEE cells in the laryngotrachea suggest that NEE cells play important roles in the defense and repair of the ciliomucous epithelium, such as increasing mucous secretion, ciliary beat frequency and the proliferation of epithelial cells.

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“…Several morphological studies indicate that the bioactive substances are released from the DCVs by exocytosis (for review, see Scheuermann 1987Scheuermann , 1997. Therefore, the position of the DCVs at the basal pole near the blood capillaries and in contact with the cell membrane suggests a potential release of their content into the circulation or into surrounding tissues.…”

Section: Discussionmentioning

confidence: 99%

Neuroepithelial bodies in the lung of Melanophryniscus stelzneri stelzneri (Anura, Bufonidae)

Hermida

Farías²,

Fiorito

2003

Anatomy and Embryology

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Electron microscopy of the lungs of Melanophryniscus stelzneri stelzneri (Anura) revealed the presence of a complex pattern of corpuscular cells (CCs). The respiratory surface over the septa presents small areas where the CCs are grouped forming neuroepithelial bodies (NEBs). These corpuscular structures can also be localized in the inner layer of the lung wall. Although in both cases NEBs protrude slightly into the airway lumen, they are separated from the airway lumen and the basal connective tissue by thin apical and basal cytoplasmic processes of neighbouring pneumocytes. Ultrastructurally, the CCs possess a large nucleus, clear cytoplasm and vesicles of variable morphology and size, containing an electron dense material surrounded by a lucent space in some cases. The size of these dense-core vesicles (DCVs) ranged from 40 to 100 nm. The NEBs are associated with afferent and efferent terminal nerves. These types of nerve endings are located between the CCs and in the basal part of the NEBs. The location of the NEBs in strategic positions on the septa and in the wall of the lung, the presence of the DCVs in the basolateral region of the CCs, the occurrence of synaptic contacts between nerve endings and the CCs and the occurrence of capillaries close to the NEBs, suggest a receptosecretory function for NEBs in the lung of M.s. stelzneri.

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Comparative histology of pulmonary neuroendocrine cell system in mammalian lungs

Cited by 9 publications

References 95 publications

Activation of mouse bronchopulmonary C‐fibres by serotonin and allergen‐ovalbumin challenge

Activation of mouse bronchopulmonary C‐fibres by serotonin and allergen‐ovalbumin challenge

Structure of the respiratory tract of the red-bellied newt Cynops pyrrhogaster, with reference to serotonin-positive neuroepithelial endocrine cells

Neuroepithelial bodies in the lung of Melanophryniscus stelzneri stelzneri (Anura, Bufonidae)

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