Comparative in vitro seed germination and seedling development in tropical and temperate epiphytic and temperate terrestrial orchids

Diantina, Surya; Kartikaningrum, Suskandari; McCormick, Andrea Clavijo; Millner, J. P.; McGill, C. R.; Pritchard, Hugh W.; Jayanthi, N.

doi:10.1007/s11240-020-01947-7

Cited by 23 publications

(9 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this study we selected orchid species representing different geographic ranges (tropical and temperate) and habits (epiphytic and terrestrial). These species were selected to support orchid conservation efforts in New Zealand and Indonesia, aiming to understand their morphology and ecology (Diantina et al 2020a), in vitro germination and seed development (Diantina et al 2020b), and the effects of seed storage and cryopreservation for ex situ conservation. These studied species were one tropical epiphytic orchid from Indonesia, Dendrobium strebloceras (subfamily Epidendroideae), and four temperate species from New Zealand, the endemic epiphytic orchid D. cunninghamii (Epidendroideae) and terrestrial orchids Gastrodia cunnninghamii (Epidendroideae), Pterostylis banksii (Orchidoideae) and Thelymitra nervosa (Orchidoideae).…”

Section: Introductionmentioning

confidence: 99%

“…2020a), in vitro germination and seed development (Diantina et al . 2020b), and the effects of seed storage and cryopreservation for ex situ conservation. These studied species were one tropical epiphytic orchid from Indonesia, Dendrobium strebloceras (subfamily Epidendroideae), and four temperate species from New Zealand, the endemic epiphytic orchid D .…”

Section: Introductionmentioning

confidence: 99%

“…Correlation between seed air volume (SAV) as calculated in Diantina et al . (2020) and changes in fatty acid content after ageing.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Seed viability and fatty acid profiles of five orchid species before and after ageing

et al. 2021

Self Cite

View full text Add to dashboard Cite

Changes in seed lipid composition during ageing are associated with seed viability loss in many plant species. However, due to their small seed size, this has not been previously explored in orchids. We characterized and compared the seed viability and fatty acid profiles of five orchid species before and after ageing: one tropical epiphytic orchid from Indonesia (Dendrobium strebloceras), and four temperate species from New Zealand, D. cunninghamii (epiphytic), and Gastrodia cunninghamii, Pterostylis banksii and Thelymitra nervosa (terrestrial).• Seeds were aged under controlled laboratory conditions (3-month storage at 60% RH and 20 °C). Seed viability was tested before and after ageing using tetrazolium chloride staining. Fatty acid methyl esters from fresh and aged seeds were extracted through trans-esterification, and then analysed using gas chromatography-mass spectrometry.• All species had high initial viability (>80%) and experienced significant viability loss after ageing. The saturated, polyunsaturated, monounsaturated and total fatty acid content decreased with ageing in all species, but this reduction was only significant for D. strebloceras, D. cunninghamii and G. cunninghamii.• Our results suggest that fatty acid degradation is a typical response to ageing in orchids, albeit with species variation in magnitude, but the link between fatty acid degradation and viability was not elucidated. Pterostylis banksii exemplified this variation; it showed marked viability loss despite not having a significant reduction in its fatty acid content after ageing. More research is required to identify the effect of ageing on fatty acid composition in orchids, and its contribution to seed viability loss.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Seed viability and fatty acid profiles of five orchid species before and after ageing

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, the dynamic of specific OMF and other beneficial fungal taxa in the plant rhizosphere can provide clues to understanding the ecology of fungal interactions with terrestrial orchids. Most of our understanding of the symbiotic associations of terrestrial orchids colonizing temperate forest ecosystems is from the northern hemisphere [ 17 , 18 , 19 ]. These studies have demonstrated the direct influence of the forest on the soil microbial communities and the preferences for specific mycorrhizal fungi [ 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Diversity of Root-Associated Fungi of the Terrestrial Orchids Gavilea lutea and Chloraea collicensis in a Temperate Forest Soil of South-Central Chile

Herrera

Sanhueza

Valadares

et al. 2022

JoF

View full text Add to dashboard Cite

The diversity of orchid mycorrhizal fungi (OMF) and other beneficial root-associated fungi in temperate forests has scarcely been examined. This study aimed to analyze the diversity of mycorrhizal and rhizosphere-associated fungal communities in the terrestrial orchids Gavilea lutea and Chloraea collicensis growing in high-orchid-population-density areas in the piedmont of the Andes Cordillera with native forest (Nothofagus-Araucaria) and Coastal Cordillera with an exotic plantation (Pinus-Eucalyptus) in south-central Chile. We focused on rhizosphere-inhabiting and peloton-associated OMF in a native forest (Andes Cordillera) and a mixed forest (Coastal Cordillera). The native terrestrial orchids G. lutea and C. collicensis were localized, mycorrhizal root segments were taken to isolate peloton-associated OMF, and rhizosphere soil was taken to perform the metabarcoding approach. The results revealed that Basidiomycota and Ascomycota were the main rhizosphere-inhabiting fungal phyla, showing significant differences in the composition of fungal communities in both sites. Sebacina was the most-abundant OMF genera in the rhizosphere of G. lutea growing in the native forest soil. In contrast, Thanatephorus was the most abundant mycorrhizal taxa growing in the rhizosphere of orchids from the Coastal Cordillera. Besides, other OMF genera such as Inocybe, Tomentella, and Mycena were detected. The diversity of OMF in pelotons differed, being mainly related to Ceratobasidium sp. and Tulasnella sp. These results provide evidence of differences in OMF from pelotons and the rhizosphere soil in G. lutea growing in the Andes Cordillera and a selection of microbial communities in the rhizosphere of C. collicensis in the Coastal Cordillera. This raises questions about the efficiency of propagation strategies based only on mycorrhizal fungi obtained by culture-dependent methods, especially in orchids that depend on non-culturable taxa for seed germination and plantlet development.

show abstract

“…The broad geographical distribution, the wide richness of species, and the genetic diversity distinctive of this family are matched with its rarity; most orchid species are threatened or endangered in the wild mainly due to habitat alteration or destruction, over-harvesting, and illegal trade [2,3]. Orchids are used for multiple purposes such as medicines, food [4,5], and cosmetic products [6,7].…”

Section: Introductionmentioning

confidence: 99%

Influence of Culture Conditions on In Vitro Asymbiotic Germination of Anacamptis longicornu and Ophrys panormitana (Orchidaceae)

Arcidiacono

Catalano

Motisi

et al. 2021

Plants

View full text Add to dashboard Cite

This study is the first approach to in vitro asymbiotic germination of two species of Sicilian threatened terrestrial orchids, Anacamptis longicornu and Ophrys panormitana. Seeds were collected in the wild and cultured in two different media—Orchimax medium (OM) and Murashige and Skoog (MS)—and exposed to different photoperiods and temperatures to evaluate the best conditions for the specific stages of development. The germination of A. longicornu was very high on OM (95.5%) and lower on MS medium (21.4%), whereas O. panormitana germinated only on OM medium, with significantly lower percentages (12.0%), compared with A. longicornu. This difference is caused by variation in quality and quantity of nutrients used, primarily by nitrogen source. The results show that temperature and photoperiod widely affect seed germination and development. Although further investigations on asymbiotic and symbiotic germination are needed for the improvement of conservation of Mediterranean terrestrial orchids, our results contribute to the conservation of this group of plants.

show abstract

Comparative in vitro seed germination and seedling development in tropical and temperate epiphytic and temperate terrestrial orchids

Cited by 23 publications

References 42 publications

Seed viability and fatty acid profiles of five orchid species before and after ageing

Seed viability and fatty acid profiles of five orchid species before and after ageing

Diversity of Root-Associated Fungi of the Terrestrial Orchids Gavilea lutea and Chloraea collicensis in a Temperate Forest Soil of South-Central Chile

Influence of Culture Conditions on In Vitro Asymbiotic Germination of Anacamptis longicornu and Ophrys panormitana (Orchidaceae)

Contact Info

Product

Resources

About