Influence of Culture Conditions on In Vitro Asymbiotic Germination of Anacamptis longicornu and Ophrys panormitana (Orchidaceae)

Arcidiacono, Myriam; Catalano, Caterina; Motisi, Antonio; Sajeva, Maurizio; Carimi, Francesco; Carra, Angela

doi:10.3390/plants10112543

Cited by 11 publications

(2 citation statements)

References 41 publications

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“…Tahap 5 merupakan tahap terbentuknya daun pertama. Tahap 6 merupakan tahap pemanjangan daun serta tahap 7 merupakan tahap terbentuknya daun kedua dan diikuti oleh pembentukan akar (Arcidiacono et al, 2021).…”

Section: Persentase Tahap-tahap Perkembangan Embriounclassified

EFFECT OF ADDING PEPTONE IN ACCELERATING GERMINATION OF Grammatophyllum stapeliiflorum J.J. Smith IN VITRO

Media,

Noli,

Suwirmen

2024

JAT

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Grammatophyllum stapeliiflorum is listed in Appendix II of the CITES. The population of this orchid has decreased due to human activities and germination in nature is low. The tissue culture technique is one solution for propagating orchids using seeds as a source of explants. Pepton is an organic compound that can increase germination and protocorm development because containing amino acid, protein biotin, pyridoxine, thiamin and nitrogen. The research was conducted to determine the effect of peptone on seed germination and protocorm development of G. stapeliiflorum through tissue culture technique. The method used a Completely Randomized Design (CRD) with 6 treatments and 4 replications. The mature seed from sterilized capsule was sown on MS mediumwithout peptone as a control treatment and added with various concentrations of peptone (0.5; 1; 1.5; 2; and 2.5 g/L). The statistical analysis results showed that peptone had a significant effect in accelerating germination. The highest percentage of seed germination (88.59%), protocorm formation (61.02%), and protocorm with SAM and RAM (22.43%) was observed in MS medium containing 2 g/L peptone.

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Section: Persentase Tahap-tahap Perkembangan Embriounclassified

EFFECT OF ADDING PEPTONE IN ACCELERATING GERMINATION OF Grammatophyllum stapeliiflorum J.J. Smith IN VITRO

Media,

Noli,

Suwirmen

2024

JAT

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“…C. maritima is a nitrophilous plant growing in areas subject to intense disturbance, habitat disruption, and urbanization; prone to coastal erosion; and degraded by human pressure owing to seasonal disturbances connected with seaside tourism [8]. In such critical situations, in vitro propagation techniques offer a valid biotechnological tool for plant conservation and production [9,10]. Micropropagation plays a key role in the production and ex situ conservation of rare, endemic, and endangered plants [11,12].…”

Section: Introductionmentioning

confidence: 99%

Propagation of Calendula maritima Guss. (Asteraceae) through Biotechnological Techniques for Possible Usage in Phytotherapy

et al. 2022

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The genus Calendula (Asteraceae) includes several species that are renowned for their therapeutic properties and/or use as ingredients in the preparation of cosmetics. The rare and critically endangered sea marigold, Calendula maritima Guss., an endemic species from Western Sicily, has also been recognized as a potential “farm plant species” for several important compounds used in cosmetics. However, the few remnant populations of this species are currently threatened with extinction because of several factors, such as hybridization with the congeneric species Calendula suffruticosa subsp. fulgida (Raf.) Guadagno and anthropogenic disturbance of its habitat. Therefore, in order to preserve the genetic integrity from pure genetic lineages, seed-based propagation and seed storage are not recommended for either conservation or massive production purposes. In this paper, we describe a protocol adopted for mass propagation of C. maritima from selected genotypes. Nodal segments collected from selected plants growing in the field were used as starting explants and were cultured for micropropagation on MS medium with and without phloroglucinol. New shoots produced were cultured for rooting under several conditions with the aim of finding the best procedure favoring root induction. The best rooting performance was obtained with zeolite and rooted plants were successfully acclimatized outdoors. The technique described allowed the multiplication of genotypes of interest as well as to overcome the problems of hybridization of this species, hence contributing concretely to the conservation of the sea marigold.

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Asymbiotic Seed Germination in Terrestrial Orchids: Problems, Progress, and Prospects

Anghelescu,

Vafaee,

Ahmadzadeh

et al. 2023

Advances in Orchid Biology, Biotechnology and Omics

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Influence of Culture Conditions on In Vitro Asymbiotic Germination of Anacamptis longicornu and Ophrys panormitana (Orchidaceae)

Cited by 11 publications

References 41 publications

EFFECT OF ADDING PEPTONE IN ACCELERATING GERMINATION OF Grammatophyllum stapeliiflorum J.J. Smith IN VITRO

EFFECT OF ADDING PEPTONE IN ACCELERATING GERMINATION OF Grammatophyllum stapeliiflorum J.J. Smith IN VITRO

Propagation of Calendula maritima Guss. (Asteraceae) through Biotechnological Techniques for Possible Usage in Phytotherapy

Asymbiotic Seed Germination in Terrestrial Orchids: Problems, Progress, and Prospects

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