Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk

Adebayo-Tayo, Bukola Christianah; Agidigbi, O.; Alao, Solomon Omoniyi

doi:10.15547/tjs.2017.01.006

Cited by 7 publications

(7 citation statements)

References 34 publications

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“…It was observed that as the exposure time increases and acridine orange dye concentration increases, the colony forming units decreases. Reduction in colony forming units is in line with the work of Adebayo-Tayo et al (2017), in which decrease in colony forming units of Lactobacillus plantarum when exposed to UV light was observed and reported. Four and two mutant strains were obtained from B. subtilis and B. megaterium respectively, when exposed to UV radiation at different time intervals, ranging from 0 to 35 minutes and acridine orange dye at different concentration ranging from 0 to 150 mg/mL.…”

Section: Mutagenesis Study Of Bacillus Speciessupporting

confidence: 90%

L-glutamic acid production by immobilized wild and mutant Bacillus species

Durojaye¹,

Adebayo-Tayo²,

Onifade³

2023

J. Biosci. Biotechnol. Discov.

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The effect of Ultra-Violet (UV) irradiation and acridine orange dye on L-glutamic acid producing Bacillus subtilis and B. megaterium was investigated. The selected mutant strains and wild types of B. subtilis and B. megaterium were immobilized and used for L-glutamic acid production. The Bacillus strains were exposed to UV irradiation and treated with acridine orange dye respectively. The survived Bacillus strains were found to reduce as the exposure time and concentration of the mutagens increased in this study. Four mutants of B. subtilis and two mutants of B. megaterium were selected for L-glutamic acid production. Mutant strain of B. subtilis (MAIR4) treated with acridine orange dye yielded the highest L-glutamic acid (4.62 mg/mL) at 72 hours. L-glutamic acid production by mutant wild and strains of B. subtilis and B. megaterium immobilized on sodium alginate, Agar-agar matrix and poly urethane foam ranged from 1.65 to 4.03 mg/mL, 2.04 to 3.98 mg/ mL and 1.89 to 3.39 mg/mL, respectively with B. megaterium (MUSO17) on sodium alginate producing the highest L-glutamic acid. Sodium alginate was the best supporting matrix for the production of L-glutamic acid in this research. Immobilization of mutant strains of Bacillus megaterium (MASO17) exposed to UV irradiation using sodium alginate supported the L-glutamic acid production.

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Section: Mutagenesis Study Of Bacillus Speciessupporting

confidence: 90%

L-glutamic acid production by immobilized wild and mutant Bacillus species

Durojaye¹,

Adebayo-Tayo²,

Onifade³

2023

J. Biosci. Biotechnol. Discov.

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“…The EPSWLD differ from EPSMLD in three peaks less in the regions 1, 236.41 cm À1 ; 1,274.99 cm À1 ; 2,928. 04-2929.97 cm À1 which indicates lesser OH presence in the EPSWLD as this region is characteristics of alcohol and aromatic bonds (Adebayo-Tayo et al, 2017. Absence of peaks in the range of 260-290 cm À1 clearly indicated that the sample did not have any proteins and nucleic acids (Bai et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

In vitro antioxidant, antibacterial, in vivo immunomodulatory, antitumor and hematological potential of exopolysaccharide produced by wild type and mutant Lactobacillus delbureckii subsp. bulgaricus

Adebayo-Tayo

Fashogbon

2020

Heliyon

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Biological evaluation of exopolysaccharides (EPS) produced by wild type and mutant Lactobacillus delbureckii (EPSWLD and EPSMLD) was investigated. Varying degrees of functional groups associated with polysaccharides were present thus confirming the EPS. The EPSs had strong antioxidant potential in a dose dependent (0.5-10 mg/ mL) manner. EPSWLD and EPSMLD exhibited the highest 1,1-diphemy 1-2-picryl-hydrazyl (DPPH) activity (73.4 % and 65.6 %), total antioxidant activity (1.80 % and 1.42 %), H 2 O 2 scavenging activity (88.5 % and 78.6 %) and Ferric Reducing Antioxidant Power (FRAP) (1.89 % and1.81 %) at 10 mg/mL respectively. WLD and MLD were highly susceptible to chloramphenicol, cotrimoxazole, tetracycline, erythromycin and ceftazidine and resistant to cefuroxime, gentamicin and cloxacillin. The EPSs had antibacterial activity against the test pathogens. B. subtilis and S. aureus had the highest susceptibility (26.0 mm and 23.0 mm). EPSMLD modulate the highest IgG, IgA and IgM production (68-126 mg/dL and 67-98 mg/dL and 64-97 mg/dL) in the treated tumor induced mice (TTIM). EPSWLD and EPSMLD exhibited reduction capability on the CEA level (3.99-4.35 ng/L and 4.12-4.23 ng/L) of the TTIM. EPSWLD TTIM had the highest amount of RBC, WBC and PCV (5.6 Â 10 12 %, 68000% and 42%). The EPS increased the lifespan of TTIM. In conclusion EPSWLD and EPSMLD had strong biological potential with pharmacological and neutraceutical activity.

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“…The EPSWWC differ from EPSMWC in four peaks less in the regions 453.29–908.50 cm 1 , 1236.41–1641.48 cm 1 and 2061.97–3398.09 cm 1 . This indicates lesser OH presence in the EPSWWC as this region is characteristics of alcohol and aromatic bonds [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains

Adebayo-Tayo

Ishola

Oyewunmi

2018

Biotechnology Reports

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HighlightsCharacterization, antioxidant and immunomodulatory potential of exopolysaccharide (EPS) produced by wild and mutant Weissella confuse was evaluated.Wild Weissella confusa (WWCEPS) produced the highest EPS compare to the mutant strain.The EPS were heteropolysaccharides containing eight (8) monosaccharides in which galactose had the highest composition (34.60 mg/g and 33.47 mg/g EPS) in wild Weissella confusa EPS (WWCEPS) and mutant Weissella confusa EPS (MWCEPS).WWCEPS and MWCEPS had antioxidant capacity and WWCEPS had the highest DPPH capacity, total antioxidant activity, hydrogen peroxide and reducing power activity (71%, 1.88%, 86.7% and 1.85%).Wild and mutant Weissella confusa EPS exhibited immunomodulatory activity on the treated mice.

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Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk

Cited by 7 publications

References 34 publications

L-glutamic acid production by immobilized wild and mutant Bacillus species

L-glutamic acid production by immobilized wild and mutant Bacillus species

In vitro antioxidant, antibacterial, in vivo immunomodulatory, antitumor and hematological potential of exopolysaccharide produced by wild type and mutant Lactobacillus delbureckii subsp. bulgaricus

Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains

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