Comparative insights using the molecular homology model of BDNF (Brain derived neurotrophic factor) of Varanus komodoensis and the known NGF (Nerve growth factor) structure of Naja atra

Verma, Ankit; Sharma, Dhirendra Kumar; Sarma, Rituparna; Chetia, Hasnahana; Saikia, Juri

doi:10.6026/97320630009755

Cited by 2 publications

(1 citation statement)

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“…The Brain-Derived Neurotrophic Factor (BDNF) is a neurotrophin family member, one of the neurotrophic factors, and a secretion protein. It increases the differentiation speed of neuronal populations of central and peripheral nervous systems ( Verma, Sharma, Sarma, Chetia, & Saikia, 2013 ). It plays a critical role in regulating neuronal survival, as well as maintenance, development, the regulation of the formation, storage, and cognition of memories.…”

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confidence: 99%

The Effects of Hesperidin on BDNF/TrkB Signaling Pathway and Oxidative Stress Parameters in the Cerebral Cortex of the Utero-placental Insufficiency Fetal Rat Model

Abdollahi¹,

Edalatmanesh²,

Hosseini³

et al. 2021

BCN

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Introduction: Uteroplacental insufficiency (UPI) produces critical neurodevelopmental problems affecting the intrauterine growth restricted (IUGR) in offspring. In this study, it was aimed to investigate the possible neuroprotective roles of hesperidin (Hes) on the fetal cerebral cortex of the UPI rat model. Methods: In this experimental study, 40 pregnant Wistar rats (age:~40-day old, weight: 180±10g) were randomly divided into 5 groups (n=8), includeing control (normal saline, orally), UPI+NS (uterine vessel ligation+normal saline, orally), UPI+HES25, UPI+HES50 and UPI+HES100 (uterine vessel ligation+25, 50 and 100 mg/kg hesperidin, orally). After being anesthetized by ketamine and xylazine, UPI was induced by permanent bilateral closure of the uterine vessels on gestation day (GD) 18. From GD15, the Hes/NS- treated groups received Hesperidin/normal saline until GD21. On GD21, uterus, placenta and fetus were dissected out and weighed. The oxidative stress parameters, including catalase (CAT) activity, malondialdehyde (MDA), and total antioxidant capacity (TAC) in the fetal cerebral cortex were measured. The expression of brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrkB) was assessed by RT qPCR methods. Data were analyzed by ANOVA and Tukey’s post hoc test. Results: Findings showed a significant difference in the uterine and fetus weight in Hes-treated mothers (p< 0.05). In fetus, Hes reduced MDA, and increased CAT activity and TAC (p ˂ 0.001 in the UPI+Hes100 group compared to the UPI+NS group). UPI reduced BDNF and TrkB mRNA expression compared to the control group (p< 0.05). Also, Significant increases in BDNF and TrkB mRNA expression were observed after administration of Hes in the fetal cerebral cortex of the UPI rat model, in a dose dependent manner (p< 0.05). Conclusion: Hesperidin; as a neuroprotective and antioxidant agent; accelerates BDNF-TrkB signaling pathway and suppresses oxidative stress parameters in the cerebral cortex of the UPI rat model.

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Section: Introductionmentioning

confidence: 99%

The Effects of Hesperidin on BDNF/TrkB Signaling Pathway and Oxidative Stress Parameters in the Cerebral Cortex of the Utero-placental Insufficiency Fetal Rat Model

Abdollahi¹,

Edalatmanesh²,

Hosseini³

et al. 2021

BCN

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Sarma¹,

Sharma²,

Internationals³

2015

JBPR

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Superoxide dismutase (SOD) responsible for dismutation of ROS produced in cell controls the aging and longevity of animals. An attempt has been made to report on sequence to structure analysis of the genes and proteins of SOD1 and SOD2 of freshwater turtles, Pelodiscus sinensis and Mauremys reevesii. Analysis of gene and protein sequences of these SODs retrieved from the NCBI database suggested that the there were minor variations in their molecular weight of the gene sequences, melting temperature, folding, aliphatic index and isoelectric point. Gene sequences were all AT rich with 5 restriction sites each in SOD1 of both the turtles and SOD2 of Pelodiscus sinensis while 8 restriction sites in SOD2 of Mauremys reevesii were obtained. SOD1 were dominated by b Strands, whereas, SOD2 were by the alpha helices. Homology models were generated by MODELLER 9.12 presented that all the models of SODs within acceptable range. Solvent accessible surface area (SASA) and active sites analysis of refined models of the SOD proteins were acidic and with 5 to 11 number of active sites in all the proteins and high percentage of exposed aliphatic residues. Therefore, it could well be inferred that these models have the potentiality to be used for understanding the aging process.

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Comparative insights using the molecular homology model of BDNF (Brain derived neurotrophic factor) of Varanus komodoensis and the known NGF (Nerve growth factor) structure of Naja atra

Cited by 2 publications

References 27 publications

The Effects of Hesperidin on BDNF/TrkB Signaling Pathway and Oxidative Stress Parameters in the Cerebral Cortex of the Utero-placental Insufficiency Fetal Rat Model

The Effects of Hesperidin on BDNF/TrkB Signaling Pathway and Oxidative Stress Parameters in the Cerebral Cortex of the Utero-placental Insufficiency Fetal Rat Model

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