Comparative investigation of the long non-coding M-F genome region of wild-type and vaccine measles viruses

Heider, Alla; Santibanez, Sabine; Tischer, Annedore; Gerike, E; Тихонова, Н. Т.; Ignatyev, G. M.; Mrazova, M.; Enders, Greg H.; Schreier, E.

doi:10.1007/s007050050259

Cited by 19 publications

(17 citation statements)

References 12 publications

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“…The unique features of the region between the M and F proteins of morbilliviruses have raised questions about its function in viral replication and virulence (2,6,7,10,15,25). In this study, we present evidence that the amino-terminal extension (residues 1 to 135) of the CDV F protein is more than a classical signal sequence.…”

Section: Discussionmentioning

confidence: 77%

Amino-Terminal Precursor Sequence Modulates Canine Distemper Virus Fusion Protein Function

Messling

Cattaneo

2002

J Virol

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The fusion (F) proteins of most paramyxoviruses are classical type I glycoproteins with a short hydrophobic leader sequence closely following the translation initiation codon. The predicted reading frame of the canine distemper virus (CDV) F protein is more complex, with a short hydrophobic sequence beginning 115 codons downstream of the first AUG. To verify if the sequence between the first AUG and the hydrophobic region is translated, we produced a specific antiserum that indeed detected a short-lived F protein precursor that we named PreF 0 . A peptide resulting from PreF 0 cleavage was identified and named Pre, and its half-life was measured to be about 30 min. PreF 0 cleavage was completed before proteolytic activation of F 0 into its F 1 and F 2 subunits by furin. To test the hypothesis that the Pre peptide may influence protein activity, we compared the function of F proteins synthesized with that peptide to that of F proteins synthesized with a shorter amino-terminal signal sequence. F proteins synthesized with the Pre peptide were more stable and less active. Thus, the Pre peptide modulates the function of the CDV F protein. Interestingly, a distinct two-hit activation process has been recently described for human respiratory syncytial virus, another paramyxovirus.

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Section: Discussionmentioning

confidence: 77%

Amino-Terminal Precursor Sequence Modulates Canine Distemper Virus Fusion Protein Function

Messling

Cattaneo

2002

J Virol

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“…The isolates Buk 88, Gag 88, and Il 88 [Tikhonova et al, 1992], and the isolates Nov 1/96, 2/96, and 3/96, show that genotype A has circulated in the European as well as in the Asiatic part of Russia during the past 10 years. Phylogenetic analysis using a part of the noncoding M-F genomic region [Heider et al, 1997] also demonstrated that these Russian isolates belonged to genotype A. Observations of other affected groups also indicate that genotype A has been present in Russia and other regions of the world within the past 10 years. The sequence published by Jin et al [1996] for the Loss strain isolated in Russia in 1988 corresponds to genotype A.…”

Section: Resultsmentioning

confidence: 87%

Genotyping of measles virus isolates from Central Europe and Russia

Santibanez

Heider²,

Gerike

et al. 1999

J. Med. Virol.

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Sequence analysis of 285 nucleotides located on the variable part of the N gene was undertaken on measles virus (MV) samples collected from acutely infected patients in Germany, the Czech Republic, Denmark, Poland, and Russia. Two distinct genotypes (C2 and D6) have circulated in Germany between 1993 and 1996. Isolates of genotype C2 were related to strains reported in Germany before 1993. This genotype was also found in the Czech Republic in 1992 and in Denmark in 1997. The occurrence of genotype D6 in Germany is described below for the first time. In 1998, this genotype was identified in Poland. Genotypes C2 and D6 were also reported in Spain and in the United Kingdom between 1992 and 1996. Therefore, it is concluded that these genotypes are widely distributed over Europe. The analysis of the isolates from Russia revealed that genotype A was present in 1988 in the European part of the country and in 1996 in Siberia. An isolate identified in 1997 in Siberia belonged to genotype D6, which had never been found previously in Russia. We also analysed MV obtained from a case of subacute sclerosing panencephalitis (SSPE) in 1995 in Turkey. A comparison of this sequence with published sequences implied that this SSPE case was associated with a new genetic lineage of MV.

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“…Heider et al [36] compared only a part (from 4725 to 5024 nt) of the long noncoding M-F genome region of wild-type (different genotypes) and measles vaccine strains [36]. Results of their analysis have shown a significant difference between the genotypes in this part of measles genome, but a high degree of conservation within each genotype.…”

Section: Resultsmentioning

confidence: 96%

A comparison of complete untranslated regions of measles virus genomes derived from wild-type viruses and SSPE brain tissues

et al. 2006

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We compared complete untranslated regions (UTRs) of two subacute sclerosing panencephalitis (SSPE) measles virus (MV) strains and two wild-type (wt) MV strains, all belonging to the same genotype (D6). In comparison to wt MVs of the same genotype, base changes were identified in the two SSPE measles virus strains at 27 and 33 noncoding positions, respectively. Majority of these residues are unique for each of the SSPE virus sequences in comparison to all other reported measles virus strain sequences. The location of some of these changes indicates that they may modify cis-acting regulatory sequences including gene-end signal of the P gene, H/L gene junction and Kozak consensus element of the L gene. Further, within the long UTR between M and F genes, deletions and insertions were identified. Thus, our study could be significant for additional investigation using reverse genetics and recombinant viruses, of possible influence of mutations in UTRs on establishment and maintenance of chronic progressive CNS disease caused by MV persistence.

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Comparative investigation of the long non-coding M-F genome region of wild-type and vaccine measles viruses

Cited by 19 publications

References 12 publications

Amino-Terminal Precursor Sequence Modulates Canine Distemper Virus Fusion Protein Function

Amino-Terminal Precursor Sequence Modulates Canine Distemper Virus Fusion Protein Function

Genotyping of measles virus isolates from Central Europe and Russia

A comparison of complete untranslated regions of measles virus genomes derived from wild-type viruses and SSPE brain tissues

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