Comparative Investigation on Radical Scavenging Activity and Protective Properties of Natural Isolated and Synthetic Antioxidants

Karamalakova, Yanka; Sharma, Jyoti; Sharma, Rakesh; Gadjeva, V.; Kumar, Raj; Zheleva, A.

doi:10.5504/50yrtimb.2011.0032

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…To measure the antioxidant activity of the woundwort extracts, four methods with different mechanisms of action were applied: (i) DPPH Radical Scavenging Assay: The DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity according to Cuendet et al [22] with slight modifications [23]; (ii) ABTS •+ assay: The radical-scavenging activity of S. virgaurea L. extracts against 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) was determined by Re et al [24] with modifications by Adhikari et al [25]; (iii) Ferric Reducing Power/electron donation (FRAP) assay: The FRAP assay was performed according to a previously reported method with a slight modification [26,27]; (iv) NO assay: The presence a stable oxidized product as a rsults of nitric oxide ion scavenging of the extracts (50 µg/mL) was performed according to the methodology of Shirwaikar et al [28] with modification by Karamalakova et al [29].…”

Section: In Vitro Antioxidant Activitymentioning

confidence: 99%

Flavonoids Extraction Kinetics, Antimicrobial Activity and Radical Scavenging Potential of Bulgarian Woundwort (Solidago virgaurea L.)

et al. 2022

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The medicinal plant woundwort (Solidago virgaurea L.) characterizes by diuretic, antimutagenic, anti-inflammatory activity and it has been applied for urinary tract, nephrolithiasis and prostate disorders treatment. The aim of the present study was to analyze the extraction kinetics of catechin, epigallocatechin and quercetin from Bulgarian woundwort extracts, to assess the antibacterial potential of the medicinal plant extracts against four bacterial strains (Staphylococcus aureus ATCC25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Bacillus cereus), their antioxidant capacity and radical scavenging potential. The concentrations of the flavonoids in the extracts obtained at different extraction conditions (solvent, temperature, extraction time) were determined by newly-developed by the scientific team RP-HPLC-PDA methodologies. The agar well diffusion method was applied to evaluate the antibacterial activity of the plant extracts. The 70% EtOH extracts at 20 °C displayed significantly higher antibacterial activity against the foodborne pathogenic bacteria S. aureus and P. aeruginosa as compared to the 70% and 98% EtOH extracts at 30 °C and 20 °C, respectively. The medicinal plant exhibited satisfactory antioxidant potential and radical-scavenging activity.

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Section: In Vitro Antioxidant Activitymentioning

confidence: 99%

Flavonoids Extraction Kinetics, Antimicrobial Activity and Radical Scavenging Potential of Bulgarian Woundwort (Solidago virgaurea L.)

et al. 2022

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“…Several reports have demonstrated the usefulness of in vitro EPR spectroscopy in determining radical capture, radioprotective (especially after UV/γ irradiation) and oxidative stress modulating activity of natural extracts, oils, etc. [16,17]. EPR spectroscopy is an accurate method for studying and evaluating redox mechanisms, and antioxidant capacity, as well as for analyzing the quality, stability, and shelf life of natural plant extracts isolated from leaves, flowers, and fruits that are supposed to have antioxidant properties [18 -20].…”

Section: Introductionmentioning

confidence: 99%

EPR spectroscopy investigation on the antioxidant efficacy of <i>Sambucus Nigra</i> L (dry flowers/blossom) extract

Petkova-Parlapanska,

Diankov,

Karsheva

2023

JCTM

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The present study aims to evaluate the antioxidant efficacy and the lipid peroxidation degree of Sambucus nigra L. (S. nigra) blossom extract after induction of biotic stress. Dry stained extracts of S. nigra flowers were analyzed by direct and indirect in vitro EPR spectroscopy. The antioxidant activity of the extract was assessed by DPPH analysis, and the inhibition of lipid peroxidation -by PBN-spin adduction, after UV-B and thermal stress. The direct EPR stable radical structures presence was established either in non-irradiated and UV-B irradiated samples. Furthermore, as before and after UV-B and thermal biotic stress, S. nigra showed well-expressed scavenging abilities and protective properties against reactive oxygen and nitrogen species such as superoxide radicals (•O 2 ̅ ), hydroxyl radicals (•OH), and reduced lipid peroxidation.

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“…The nitroxyl radicals having only one functional group are substances with relatively low acute toxicity. It has been shown that nitroxides at non-toxic concentrations are effective both in vitro and in vivo [19,20].…”

mentioning

confidence: 99%

Options for reducing oxidative toxicity of L-dopa by combination with synthetic or natural radical protectors

Nikolova¹

2018

TJS

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Parkinson disease (PD) is a multifactorial disease that is not well-established. It takes a leading place among contemporary frequent diseases of the central nervous system (CNS). The levodopa (L-dopa) clinical effect is diminished by motor complications resulting from prolonged treatment. Due to the L-dopa neurotoxic effect in the disease treatment, the L-dopa administration is delayed as long as possible in order to avoid side effects. In addition, combining L-dopa therapy with antioxidants (from natural or synthetic origin), may decrease side-effects and provide symptomatic relief. The aim of the current research is through experimental model of healthy mice to explore the possibility to reduce the oxidative stress (OS) induced by the L-dopa drug after its combining with: an essential oil isolated from Rosa damascena Mill., the vitamin C and synthetic antioxidant 1-ethyl-3- [4- (2,2,6,6-tetramethylpiperidine-1-oxyl)] - 1-nitrosourea SLENU. The antioxidants protective effects against the L-dopa oxidative toxicity were evaluated through the oxidative stress indicators - the lipid and protein oxidation end products – measured as MDA, protein carbonyl content, and advanced glycation end products (AGEs) in blood plasma of experimental mice.

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Comparative Investigation on Radical Scavenging Activity and Protective Properties of Natural Isolated and Synthetic Antioxidants

Cited by 4 publications

References 15 publications

Flavonoids Extraction Kinetics, Antimicrobial Activity and Radical Scavenging Potential of Bulgarian Woundwort (Solidago virgaurea L.)

Flavonoids Extraction Kinetics, Antimicrobial Activity and Radical Scavenging Potential of Bulgarian Woundwort (Solidago virgaurea L.)

EPR spectroscopy investigation on the antioxidant efficacy of <i>Sambucus Nigra</i> L (dry flowers/blossom) extract

Options for reducing oxidative toxicity of L-dopa by combination with synthetic or natural radical protectors

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