Comparative investigations on the antagonistic activity of fluorescent pseudomonads against Gaeumannomyces graminis var. tritici in vitro and in vivo

Elsherif, Mohamed; Grossmann, F.

doi:10.1016/s0944-5013(11)80084-4

Cited by 19 publications

(9 citation statements)

References 33 publications

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“…In vitro inhibition of pathogens by microbes on agar plates is a common method used for preliminary screening of potential biocontrol agents, however, there often is a poor correlation between in vitro inhibition and disease control (Castejón-Muñoz & Oyarzun, 1995, Elsherif & Grossmann, 1994, Knudsen et al, 1997, Reddy et al, 1994. Even so, in vitro inhibition studies are still used as initial selection criteria (Dua & Sindhu, 2012, Mavrodi et al, 2012b.…”

Section: Discussionmentioning

confidence: 99%

Selection of microbes for control of Rhizoctonia root rot on wheat using a high throughput pathosystem

et al. 2017

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Please cite this article as: Barnett, S., Zhao, S., Ballard, R., Franco, C., Selection of microbes for control of Rhizoctonia root rot on wheat using a high throughput pathosystem, Biological Control (2017), doi: http://dx.One sentence summary: A 3-phase in planta pathosystem was used to screen large numbers of plant-associated microbes resulting in the selection of candidate strains for control of Rhizoctonia root rot on wheat. AbstractThe promise of microbial biological control of soilborne fungal pathogens of crops has yet to be fully realised with only a few strains commercialised and available to growers. One bottleneck is the availability of suitable methods to screen microorganisms for disease control efficacy relevant to controlling disease in the field. A 3-phase in planta pathosystem containing field soil was developed to screen 2,310 microorganisms for control of Rhizoctonia root rot on wheat. Test strains were added to seeds as a suspension at planting and plant growth assessed at two weeks. Strains increasing plant height and number of roots (185) were tested in a replicated Rhizoctonia pot bioassay with five wheat seedlings grown for four weeks and assessed for plant growth and root disease. Forty three strains (1.9% of strains tested) performed better than our benchmark strains and were reassessed in pot bioassays at three inoculation levels. These tested strains represented a wide diversity of microbial genotypes including fungi, (Trichoderma, Aspergillus and Cylindrocarpon) and bacteria encompassing four phyla (Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes).These results show that microbes can be successfully and rapidly screened directly for disease control on plants.

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Section: Discussionmentioning

confidence: 99%

Selection of microbes for control of Rhizoctonia root rot on wheat using a high throughput pathosystem

et al. 2017

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“…Some of the fungi that have been found to be effective are closely related to the take‐all fungus, namely avirulent/hypovirulent isolates of G. graminis and Phialophora species (Andrade et al ., 1994; Duffy and Weller, 1995, 1996; Mathre et al ., 1998; Sivasithamparam, 1975; Wong et al ., 1996; Zriba et al ., 1999). The accumulated research on potential biological control agents for take‐all of wheat demonstrates that screening in vitro , and even in pot experiments, does not necessarily give a reliable indication of effectiveness in the field (Cook et al ., 1995; Elsherif and Grossmann, 1994). It is also evident that agents that give control in one geographical location do not necessarily have any beneficial effects elsewhere (e.g.…”

Section: Take‐all Disease Of Wheat and Its Controlmentioning

confidence: 99%

Gaeumannomyces graminis, the take‐all fungus and its relatives

Freeman

Ward

2004

Molecular Plant Pathology

105

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SUMMARY Take‐all, caused by the fungus Gaeumannomyces graminis var. tritici, is the most important root disease of wheat worldwide. Many years of intensive research, reflected by the large volume of literature on take‐all, has led to a considerable degree of understanding of many aspects of the disease. However, effective and economic control of the disease remains difficult. The application of molecular techniques to study G. graminis and related fungi has resulted in some significant advances, particularly in the development of improved methods for identification and in elucidating the role of the enzyme avenacinase as a pathogenicity determinant in the closely related oat take‐all fungus (G. graminis var. avenae). Some progress in identifying other factors that may be involved in determining host range and pathogenicity has been made, despite the difficulties of performing genetic analyses and the lack of a reliable transformation system.

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“…iii) Increasing the amount of iron present in the soil to 40 µmol Fe +3 /L caused a parallel decrease in both the amount of fluorescent siderophores produced and the inhibitory effect against Gaeumannomyces graminis var. tritici, a pathogen of wheat, in a collection of 70 separate isolates of fluorescent pseudomonads ( Elsherif and Grossmann, 1994). iv).…”

Section: Siderophoresmentioning

confidence: 99%

Genetic manipulation of plant growth-promoting bacteria to enhance biocontrol of phytopathogens

Glick

Bashan²

1997

Biotechnology Advances

262

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Plant growth-promoting bacteria (PGPB) control the damage to plants from phytopathogens by a number of d ifferent mechanisms including: outcompeting the phytopathogen, physical displacement of the phytopathogen, secretion of siderophores to prevent pathogens in the immediate vicinity from proliferating, synthesis of antibiotics, synthesis of a variety of small molecules that can inhibit phytopathogen growth, production of enzymes that inhibit the phytopathogen and stimulation of the systemic resistance of the plant. Biocontrol PGPB may be improved by genetically engineering them to overexpress one or more of t hese traits so that strains with several different anti-phytopathogen traits which can act synergistically are created. In engineering these strains it is essential to ensure that the normal functioning of the bacterium is not impaired, i.e., that there is no problem with metabolic load.

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Comparative investigations on the antagonistic activity of fluorescent pseudomonads against Gaeumannomyces graminis var. tritici in vitro and in vivo

Cited by 19 publications

References 33 publications

Selection of microbes for control of Rhizoctonia root rot on wheat using a high throughput pathosystem

Selection of microbes for control of Rhizoctonia root rot on wheat using a high throughput pathosystem

Gaeumannomyces graminis, the take‐all fungus and its relatives

Genetic manipulation of plant growth-promoting bacteria to enhance biocontrol of phytopathogens

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