Comparative metabolite profiling of two switchgrass ecotypes reveals differences in drought stress responses and rhizosheath weight

Liu, Tie Yuan; Chen, Mo Xian; Zhang, Youjun; Zhu, Fu Yuan; Liu, Ying Gao; Tian, Yuan; Fernie, Alisdair R.; Ye, Nenghui; Zhang, Jianhua

doi:10.1007/s00425-019-03228-w

Cited by 34 publications

(37 citation statements)

References 67 publications

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“…Our results showed that OAs play an important role on the first day of salt stress. Similar to that which occurred in the present study, Liu et al [66] analyzed switchgrass under drought stress and found a large amount of AA and OA accumulation. Metabolomic analysis of buckwheat under salt stress also led to the same conclusion [49].…”

Section: Oas Are Correlated With Salt Stresssupporting

confidence: 89%

Transcriptomic and metabolomic analyses reveal mechanisms of adaptation to salinity in which carbon and nitrogen metabolism is altered in sugar beet roots

et al. 2020

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Background: Beta vulgaris L. is one of the main sugar-producing crop species and is highly adaptable to saline soil. This study explored the alterations to the carbon and nitrogen metabolism mechanisms enabling the roots of sugar beet seedlings to adapt to salinity. Results: The ionome, metabolome, and transcriptome of the roots of sugar beet seedlings were evaluated after 1 day (short term) and 7 days (long term) of 300 mM Na + treatment. Salt stress caused reactive oxygen species (ROS) damage and ion toxicity in the roots. Interestingly, under salt stress, the increase in the Na + /K + ratio compared to the control ratio on day 7 was lower than that on day 1 in the roots. The transcriptomic results showed that a large number of differentially expressed genes (DEGs) were enriched in various metabolic pathways. A total of 1279 and 903 DEGs were identified on days 1 and 7, respectively, and were mapped mainly to 10 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Most of the genes were involved in carbon metabolism and amino acid (AA) biosynthesis. Furthermore, metabolomic analysis revealed that sucrose metabolism and the activity of the tricarboxylic acid (TCA) cycle increased in response to salt stress. After 1 day of stress, the content of sucrose decreased, whereas the content of organic acids (OAs) such as L-malic acid and 2-oxoglutaric acid increased. After 7 days of salt stress, nitrogen-containing metabolites such as AAs, betaine, melatonin, and (S)-2-aminobutyric acid increased significantly. In addition, multiomic analysis revealed that the expression of the gene encoding xanthine dehydrogenase (XDH) was upregulated and that the expression of the gene encoding allantoinase (ALN) was significantly downregulated, resulting in a large accumulation of allantoin. Correlation analysis revealed that most genes were significantly related to only allantoin and xanthosine. Conclusions: Our study demonstrated that carbon and nitrogen metabolism was altered in the roots of sugar beet plants under salt stress. Nitrogen metabolism plays a major role in the late stages of salt stress. Allantoin, which is involved in the purine metabolic pathway, may be a key regulator of sugar beet salt tolerance.

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Section: Oas Are Correlated With Salt Stresssupporting

confidence: 89%

Transcriptomic and metabolomic analyses reveal mechanisms of adaptation to salinity in which carbon and nitrogen metabolism is altered in sugar beet roots

et al. 2020

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“…Many studies over the past few decades have explored the response of plant roots to drought stress at the morphological, physiological, and molecular levels (Bartels and Sunkar, 2005; Xiong et al, 2006; Akpinar et al, 2015). Cereals and some other grass species form rhizosheath to ensure their successful adaptation to drought environments (George et al, 2014; Delhaize et al, 2015; Adu et al, 2017; Liu et al, 2019a; 2019b). The rhizosheath is a modification of the rhizosphere and is employed by plants as a drought tolerance strategy.…”

Section: Introductionmentioning

confidence: 99%

“…This root–soil contact surface has been described as an interesting interaction region between plants and the soil environment. The rhizosheath largely forms in drying soil and requires the presence of root hairs and mucilage to aggregate the soil particles together (Liu et al, 2019a). The mucilage is secreted from roots or bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…A growing number of studies have revealed the formation of rhizosheaths on grasses and cereals in drying soil. The rhizosheath is different from the rhizosphere in terms of its boundaries, distribution, and heterogeneity in space and time, since it has a tight outer surface and shows clear separation from bulk soil (Liu et al, 2019a). Recent studies have examined the microbial composition of the rhizosheath and its variations in the plants Stipagrostis pennata , S. sabulicola , S. seelyae , and Cladoraphis spinosa (Marasco et al, 2018; Tian et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Alamo forms a large rhizosheath, while cv. Kanlow forms a small rhizosheath under drought conditions (Liu et al, 2019a). The root phenotypes and root metabolic compositions of these two switchgrass ecotypes also differ, including the compositions of sugars and organic acids (Liu et al, 2019a).…”

Section: Introductionmentioning

confidence: 99%

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Drought stress and plant ecotype drive microbiome recruitment in switchgrass rhizosheath

Liu

Wang

et al. 2021

JIPB

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The rhizosheath, a layer of soil grains that adheres firmly to roots, is beneficial for plant growth and adaptation to drought environments. Switchgrass is a perennial C4 grass which can form contact rhizosheath under drought conditions. In this study, we characterized the microbiomes of four different rhizocompartments of two switchgrass ecotypes (Alamo and Kanlow) grown under drought or well-watered conditions via 16S ribosomal RNA amplicon sequencing. These four rhizocompartments, the bulk soil, rhizosheath soil, rhizoplane, and root endosphere, harbored both

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Bioenergy Underground: Challenges and opportunities for phenotyping roots and the microbiome for sustainable bioenergy crop production

York

Cumming

Trusiak

et al. 2022

The Plant Phenome Journal

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Bioenergy production often focuses on the aboveground feedstock production for conversion to fuel and other materials. However, the belowground component is crucial for soil carbon sequestration, greenhouse gas fluxes, and ecosystem function. Roots maximize feedstock production on marginal lands by acquiring soil resources and mediating soil ecosystem processes through interactions with the microbial community. This belowground world is challenging to observe and quantify; however, there are unprecedented opportunities using current methodologies to bring roots, microbes, and soil into focus. These opportunities allow not only breeding for increased feedstock production but breeding for increased soil health and carbon sequestration as well. A recent workshop hosted by the USDOE Bioenergy Research Centers highlighted these challenges and opportunities while creating a roadmap for increased collaboration and data interoperability through standardization of methodologies and data using F.A.I.R. principles. This article provides a background on the need for belowground research in bioenergy cropping systems, a primer on root system properties of major U.S. bioenergy crops, and an overview of the roles of root chemistry, exudation, and microbial interactions on sustainability. Crucially, we outline how to adopt standardized measures and databases to meet the most pressing methodological needs to accelerate root, soil, and microbial research to meet the pressing societal challenges of the century.

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Comparative metabolite profiling of two switchgrass ecotypes reveals differences in drought stress responses and rhizosheath weight

Abstract: Main conclusion Rhizosheath comprises soil that adheres firmly to roots. In this study, two ecotypes of switchgrass with different rhizosheath sizes after drought stress were analyzed which showed metabolic differences under drought conditions.

Cited by 34 publications

References 67 publications

Transcriptomic and metabolomic analyses reveal mechanisms of adaptation to salinity in which carbon and nitrogen metabolism is altered in sugar beet roots

Transcriptomic and metabolomic analyses reveal mechanisms of adaptation to salinity in which carbon and nitrogen metabolism is altered in sugar beet roots

Drought stress and plant ecotype drive microbiome recruitment in switchgrass rhizosheath

Bioenergy Underground: Challenges and opportunities for phenotyping roots and the microbiome for sustainable bioenergy crop production

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