Comparative metabolomic analysis on industrial continuous and batch ethanol fermentation processes by GC-TOF-MS

Abstract

The intracellular metabolic profile characterization of Saccharomyces cerevisiae throughout industrial ethanol fermentation was investigated using gas chromatography coupled to time-of-flight mass spectrometry. A total of 143 and 128 intracellular metabolites in S. cerevisiae were detected and quantified in continuous and batch fermentations, respectively. The two fermentation processes were both clearly distinguished into three main phases by principal components analysis. Furthermore, the levels of some metabolites involved in central carbon metabolism varied significantly throughout both processes. Glycerol and phosphoric acid were principally responsible for discriminating seed, main and final phases of continuous fermentation, while lactic acid and glycerol contributed mostly to telling different phases of batch fermentation. In addition, the levels of some amino acids such as glycine varied significantly during both processes. These findings provide new insights into the metabolomic characteristics during industrial ethanol fermentation processes.

Metabolomics reveals changes in metabolite concentrations and correlations during sexual development of Eurotium cristatum (synonym: Aspergillus cristatus)

“…The high sensitivity, accuracy, and throughput capacity of metabolomics technologies have allowed for the analysis of complex metabolic changes associated with morphogenesis (Bino et al 2004, Han et al 2012. Indeed, metabolomics analysis has revealed that morphological development is associated with dynamic changes in metabolite profiles and metabolite networks (Bino et al 2004, Ding et al 2009, Roze et al 2010, Han et al 2012.…”

“…The samples were processed using a global accepted mass spectrometry-based platform with GC-MS. Data processing methods were described previously (Ding et al 2009, Ding et al 2010. The original data were transformed into CDF format (NetCDF) using Agilent GC/MS 5975 Data Analysis software and processed using XCMS software (www.bioconductor.org).…”

“…The unique taste and health benefits are due to microbial metabolism during 1628 The frozen samples were ground to obtain a fine powder, and 100-mg was used for each metabolite extraction. Extraction and derivatization methods were described previously (Ding et al 2009, Ding et al 2010.…”

Suitable extracellular oxidoreduction potential inhibit

“…Then 10 uL internal standard solution succinic d 4 acid (Sigma, 0.1 mg/ml) was added into the 100 uL extract supernatants before lyophilization. After lyophilization, the derivatization and measurement by GC-MS of these samples were carried out according to a previous method [35]. Four biological replicates were performed for each sample.…”

Wine Metabolite Profiling: Possible Application in Winemaking and Grapevine Breading in Bulgaria

“…Wine composition was a subject in a number of metabolite studies during the last decade involving metabolite profiling and more recently extensive chemodiversity analysis. Most of the reported metabolite studies utilize mass spectrometry (MS) analysis following chromatographic separation, (2,9,16). The proton nuclear magnetic resonance ( 1 h nMR) profiling alone, or in parallel with the MS methods, has been also increasingly applied during the last few years, (22,25).…”