Comparative metabolomics reveals the cytotoxic and anti-inflammatory discriminatory chemical markers of raw and roasted colocynth fruit (Citrullus colocynthis L.)

Abstract

Colocynth has a long history of use in traditional medicine for treatment of various inflammatory diseases where it is commonly roasted before being applied for medical purposes to reduce its toxicity.

Inhibitory effect of phenolic extract from squirting cucumber (Ecballium elaterium (L.) A. Rich) seed oil on integrin-mediated cell adhesion, migration and angiogenesis

“…The compounds identified included 4 flavan-3-ols (catechin, catechin-3- O -rutinoside, proanthocyanidin dimer I and proanthocyanidin II), 3 flavanones (calodendroside, trihydroxyflavanone- O -deoxyhexosyl- O -hexoside and 7,3′,4′-trihydroxyflavanone), 34,35 4 phenolic acids (4-ferulyolquinic acid, shikimic acid hexoside I, shikimic acid hexoside II, and caffeoylglucaric acid), 36–38 5 fatty acids and fatty acid derviatives (trihydroxy-octadecadienoic acid, trihydroxy-octadecenoic acid, 9-/13-hydroxy-9 Z ,11 E -octadecadienoic acid and linolenic acid) 39 and 6 cucurbitacin-type triterpenes (cucurbitacin A, B, D, E, dihydro-cucurbitacin B and colocynthoside B). 39,40…”

“…The compounds identied included 4 avan-3-ols (catechin, catechin-3-O-rutinoside, proanthocyanidin dimer I and proanthocyanidin II), 3 avanones (calodendroside, trihydroxy-avanone-O-deoxyhexosyl-O-hexoside and 7,3 ′ ,4 ′ -trihydroxyavanone), 34,35 4 phenolic acids (4-ferulyolquinic acid, shikimic acid hexoside I, shikimic acid hexoside II, and caffeoylglucaric acid), [36][37][38] 5 fatty acids and fatty acid derviatives The quantitative measurement of total vessel length was performed on 50% of the total CAM surface treated in the absence or in the presence of PEO extract. For total vessel length, results are expressed as means ± SD of three experiments performed in triplicate and difference between treated and untreated (control) cells was considered statistically significant at **P < 0.01; ***P < 0.001.…”

Forecasting of potential anti-inflammatory targets of some immunomodulatory plants and their constituents using in vitro, molecular docking and network pharmacology-based analysis

“…The means ± standard deviations of three distinct replicates were used to express the results. The method described by Darwish et al 110 , 111 , as given in the supplementary data, contains the specific details about the procedures. Statistical analysis of the results was performed using One-way ANOVA test- Tukey post-test options implemented on GraphPad Prism 5 program.…”

Diagnostic and predictive values of serum metabolic profiles in sudden sensorineural hearing loss patients

“…All analyzed samples were kept at 4°C, and the temperature of the column was kept at 25°C. The analysis was performed with an elution gradient as previously described ( Darwish et al, 2021 ; Wu et al, 2022b ). The Triple TOF 6600 mass spectrometry (AB Sciex, Boston, MA, United States) was applied to catch MS/MS spectra on an information-dependent basis (IDA) during the LC/MS experiment.…”